Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WDM69_RS02635 Genome accession   NZ_CP147511
Coordinates   589781..590827 (-) Length   348 a.a.
NCBI ID   WP_078306112.1    Uniprot ID   A0A1T0CK23
Organism   Moraxella lincolnii strain 302605.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 584781..595827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDM69_RS02605 (WDM69_02605) - 585563..586768 (+) 1206 WP_338755634.1 hypothetical protein -
  WDM69_RS02610 (WDM69_02610) - 586855..587193 (+) 339 WP_078306107.1 RnfH family protein -
  WDM69_RS02615 (WDM69_02615) bamE 587298..587633 (-) 336 WP_240494802.1 outer membrane protein assembly factor BamE -
  WDM69_RS02620 (WDM69_02620) fur 587896..588330 (+) 435 WP_078306109.1 ferric iron uptake transcriptional regulator -
  WDM69_RS02625 (WDM69_02625) - 588391..588570 (+) 180 WP_078306110.1 hypothetical protein -
  WDM69_RS02630 (WDM69_02630) pilU 588637..589758 (-) 1122 WP_078306111.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WDM69_RS02635 (WDM69_02635) pilT 589781..590827 (-) 1047 WP_078306112.1 type IV pilus twitching motility protein PilT Machinery gene
  WDM69_RS02640 (WDM69_02640) - 591030..591809 (+) 780 WP_078306113.1 YggS family pyridoxal phosphate-dependent enzyme -
  WDM69_RS02645 (WDM69_02645) trmB 591813..592625 (-) 813 WP_143823209.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  WDM69_RS02650 (WDM69_02650) - 592878..593222 (+) 345 Protein_514 transposase -
  WDM69_RS02655 (WDM69_02655) - 593281..594201 (+) 921 WP_078306114.1 DUF2628 domain-containing protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38673.39 Da        Isoelectric Point: 6.7827

>NTDB_id=946559 WDM69_RS02635 WP_078306112.1 589781..590827(-) (pilT) [Moraxella lincolnii strain 302605.3]
MSQVSIEDLLRFVVKNKASDLHISAGLPAMIRVDGEVQRINMAPLEHKDVHQLIYDIMNDKQRADYEEFFETDFSFEIPK
LARFRVNAFNQNRGAGAVFRTIPSEVLTMEQLGMGDVFKKVSNFKKGVVLVTGPTGSGKSTTLAAMIDYINETRREHILT
IEDPIEFVHESKKSLVNQREVHRDTLSFDAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTI
DRVIDVFPAAEKDMIRAMLSESLQAVISQTLLKRQTGGRVAAHEIMLGTPAIRNLIRENKVAQMYSAIQTGAGEGMTTLD
QTLKNLVSKGTISKESARAYAKQPEAFV

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=946559 WDM69_RS02635 WP_078306112.1 589781..590827(-) (pilT) [Moraxella lincolnii strain 302605.3]
ATGAGTCAAGTCAGTATTGAAGATTTGTTACGCTTTGTGGTCAAAAACAAAGCTTCGGATTTGCACATTTCAGCAGGTCT
GCCTGCGATGATACGTGTCGATGGTGAAGTTCAACGCATCAACATGGCACCCCTAGAACACAAAGATGTTCATCAGCTTA
TCTATGACATCATGAACGACAAACAACGTGCAGATTATGAAGAGTTTTTTGAAACTGACTTTTCTTTTGAAATCCCTAAA
CTTGCTCGCTTTCGTGTCAACGCCTTTAACCAAAACCGTGGAGCAGGTGCGGTGTTTCGTACCATTCCATCAGAAGTATT
GACCATGGAACAACTTGGCATGGGTGATGTGTTTAAAAAAGTGTCTAACTTTAAAAAAGGCGTGGTGCTGGTTACAGGAC
CGACAGGCTCAGGCAAATCCACCACATTGGCAGCGATGATTGACTATATCAATGAGACCCGTCGTGAGCATATCTTGACC
ATTGAGGACCCCATTGAGTTTGTCCATGAATCCAAAAAAAGCTTGGTCAACCAGCGTGAAGTTCACCGTGATACCCTAAG
CTTTGATGCTGCATTACGCTCAGCTCTTCGTGAAGACCCTGACATCATCTTGGTGGGTGAGATGCGTGACCTTGAAACCA
TTCGCCTTGCTTTGACTGCTGCTGAAACAGGACACTTGGTGTTTGGCACTTTGCACACCAGCTCTGCGGCTAAAACCATT
GACCGTGTGATTGACGTGTTCCCTGCTGCCGAAAAAGACATGATACGAGCCATGCTTTCTGAGTCATTACAAGCGGTTAT
CTCACAAACCCTACTGAAACGTCAGACAGGAGGTCGGGTAGCAGCTCACGAAATCATGCTTGGCACGCCTGCCATTCGTA
ACCTTATCCGTGAAAACAAAGTTGCTCAGATGTATTCTGCCATTCAAACTGGAGCAGGCGAAGGCATGACGACGCTGGAT
CAAACCCTAAAAAACTTAGTCAGTAAAGGCACCATCAGCAAAGAATCTGCCCGCGCGTATGCCAAACAGCCTGAAGCTTT
TGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T0CK23

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.29

99.138

0.796
  pilT Acinetobacter baumannii D1279779

80

99.138

0.793
  pilT Acinetobacter baumannii strain A118

80

99.138

0.793
  pilT Pseudomonas aeruginosa PAK

79.651

98.851

0.787
  pilT Pseudomonas stutzeri DSM 10701

77.907

98.851

0.77
  pilT Acinetobacter baylyi ADP1

77.035

98.851

0.761
  pilT Legionella pneumophila strain Lp02

73.977

98.276

0.727
  pilT Legionella pneumophila strain ERS1305867

73.977

98.276

0.727
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.365

95.977

0.647
  pilT Vibrio cholerae strain A1552

67.365

95.977

0.647
  pilT Neisseria meningitidis 8013

64.431

98.563

0.635
  pilT Neisseria gonorrhoeae MS11

64.14

98.563

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.873

99.713

0.517
  pilU Pseudomonas stutzeri DSM 10701

41.003

97.414

0.399
  pilU Vibrio cholerae strain A1552

42.025

93.678

0.394
  pilU Acinetobacter baylyi ADP1

39.359

98.563

0.388