Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WDM69_RS02630 Genome accession   NZ_CP147511
Coordinates   588637..589758 (-) Length   373 a.a.
NCBI ID   WP_078306111.1    Uniprot ID   A0A1T0CKK8
Organism   Moraxella lincolnii strain 302605.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 583637..594758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDM69_RS02600 (WDM69_02600) - 584674..585339 (+) 666 WP_078306105.1 c-type cytochrome -
  WDM69_RS02605 (WDM69_02605) - 585563..586768 (+) 1206 WP_338755634.1 hypothetical protein -
  WDM69_RS02610 (WDM69_02610) - 586855..587193 (+) 339 WP_078306107.1 RnfH family protein -
  WDM69_RS02615 (WDM69_02615) bamE 587298..587633 (-) 336 WP_240494802.1 outer membrane protein assembly factor BamE -
  WDM69_RS02620 (WDM69_02620) fur 587896..588330 (+) 435 WP_078306109.1 ferric iron uptake transcriptional regulator -
  WDM69_RS02625 (WDM69_02625) - 588391..588570 (+) 180 WP_078306110.1 hypothetical protein -
  WDM69_RS02630 (WDM69_02630) pilU 588637..589758 (-) 1122 WP_078306111.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WDM69_RS02635 (WDM69_02635) pilT 589781..590827 (-) 1047 WP_078306112.1 type IV pilus twitching motility protein PilT Machinery gene
  WDM69_RS02640 (WDM69_02640) - 591030..591809 (+) 780 WP_078306113.1 YggS family pyridoxal phosphate-dependent enzyme -
  WDM69_RS02645 (WDM69_02645) trmB 591813..592625 (-) 813 WP_143823209.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  WDM69_RS02650 (WDM69_02650) - 592878..593222 (+) 345 Protein_514 transposase -
  WDM69_RS02655 (WDM69_02655) - 593281..594201 (+) 921 WP_078306114.1 DUF2628 domain-containing protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41849.99 Da        Isoelectric Point: 6.1873

>NTDB_id=946558 WDM69_RS02630 WP_078306111.1 588637..589758(-) (pilU) [Moraxella lincolnii strain 302605.3]
MDFDKLLQLMVKQNGSDLFITANVPPSIKINGQIMPVSKSPLSPKQTMQLVTSIMTNSQKQEFEETNECQFAISDKGETA
RFRVSAFIQRNHAGMVLRKIETEIPTVEDLNLPIILKELAMKKRGIILLVGATGTGKSTTLAAMMGYRNANSRGHIITIE
DPIEYVHEHKGCIVTQREVGIDTLSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLRAIIAQQLIPTPDGKGRRAAIEILLNSQLISDFIRKGEVHEIKPVMKRSREQGMQTFDQ
ALFDLYEAGEITYKDALKHADSPNDLRLQIKLEGKNAGQDIDDDNTLSLDINE

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=946558 WDM69_RS02630 WP_078306111.1 588637..589758(-) (pilU) [Moraxella lincolnii strain 302605.3]
ATGGATTTTGATAAATTATTACAATTAATGGTAAAACAAAACGGCTCAGACCTATTCATTACCGCCAATGTGCCACCATC
TATAAAAATTAATGGTCAAATCATGCCTGTGAGCAAATCACCATTATCACCCAAGCAAACCATGCAGCTGGTTACCAGCA
TCATGACCAACAGCCAAAAACAAGAATTTGAAGAGACCAATGAGTGCCAGTTTGCCATTAGCGATAAAGGTGAGACAGCA
CGCTTTCGTGTCAGTGCTTTTATCCAACGAAACCATGCTGGCATGGTGCTGCGTAAAATTGAAACAGAAATCCCCACGGT
TGAAGACTTAAATCTGCCCATCATCTTAAAAGAATTGGCGATGAAAAAACGTGGCATTATCCTATTGGTCGGAGCAACAG
GCACAGGTAAATCCACCACCCTTGCCGCCATGATGGGCTATCGCAATGCCAACTCTCGAGGACACATCATCACCATCGAA
GACCCCATTGAATACGTCCACGAACACAAAGGCTGTATCGTTACTCAACGTGAAGTGGGTATTGACACCCTATCGTTTGA
AGCGGGGCTAAAAAACACCCTGCGTCAAGCCCCAGATGTGATTTTGATTGGGGAGATTCGTAACCGTGAAGTCATGAGTT
ACGCCATTCAATATGCCGAAACAGGGCATTTGGTATTTGCCACCTTGCACGCTAACAACGCCAACCAAGCCATTGACCGC
ATCATTCACTTTTTTGAGGCCGACCGTCATAATCAACTATTTATGGACTTGTCGCTCAACTTACGTGCCATCATTGCCCA
ACAGCTGATACCCACACCAGATGGCAAAGGGCGACGAGCAGCGATTGAGATTTTGTTAAATTCACAACTTATCAGTGACT
TTATTCGTAAAGGAGAAGTCCATGAAATTAAGCCTGTGATGAAGCGTTCTCGTGAGCAGGGCATGCAAACCTTTGACCAA
GCCTTGTTTGATTTGTATGAAGCAGGTGAAATCACTTACAAAGATGCCCTCAAACACGCCGATTCACCCAATGACCTACG
CCTACAAATCAAACTAGAAGGCAAAAATGCAGGTCAAGACATAGATGATGACAACACATTGTCTTTAGACATTAATGAAT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T0CKK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.975

96.783

0.668
  pilU Acinetobacter baylyi ADP1

67.391

98.66

0.665
  pilU Vibrio cholerae strain A1552

54.416

94.102

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

92.761

0.394
  pilT Legionella pneumophila strain Lp02

44.308

87.131

0.386
  pilT Legionella pneumophila strain ERS1305867

44.308

87.131

0.386
  pilT Pseudomonas aeruginosa PAK

41.618

92.761

0.386
  pilT Acinetobacter nosocomialis M2

41.691

91.957

0.383
  pilT Acinetobacter baumannii D1279779

41.691

91.957

0.383
  pilT Acinetobacter baumannii strain A118

41.691

91.957

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.751

92.761

0.378
  pilT Acinetobacter baylyi ADP1

40.525

91.957

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.125

85.791

0.37
  pilT Vibrio cholerae strain A1552

43.125

85.791

0.37