Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V6Z91_RS12500 Genome accession   NZ_CP146614
Coordinates   2822431..2823474 (-) Length   347 a.a.
NCBI ID   WP_338770762.1    Uniprot ID   -
Organism   Massilia sp. METH4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2817431..2828474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6Z91_RS12485 - 2818050..2820536 (-) 2487 WP_338770756.1 PAS domain S-box protein -
  V6Z91_RS12490 - 2820784..2821269 (-) 486 WP_338770758.1 glutathione peroxidase -
  V6Z91_RS12495 pilU 2821277..2822413 (-) 1137 WP_338770760.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V6Z91_RS12500 pilT 2822431..2823474 (-) 1044 WP_338770762.1 type IV pilus twitching motility protein PilT Machinery gene
  V6Z91_RS12505 - 2823635..2824342 (+) 708 WP_338770763.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6Z91_RS12510 proC 2824357..2825184 (+) 828 WP_338770764.1 pyrroline-5-carboxylate reductase -
  V6Z91_RS12515 - 2825184..2825405 (+) 222 WP_338770766.1 hypothetical protein -
  V6Z91_RS12520 - 2825454..2825819 (-) 366 WP_338770768.1 DUF883 domain-containing protein -
  V6Z91_RS12525 ubiA 2825923..2826798 (-) 876 WP_338770770.1 4-hydroxybenzoate octaprenyltransferase -
  V6Z91_RS12530 - 2826808..2827743 (-) 936 WP_338770772.1 hydrogen peroxide-inducible genes activator -
  V6Z91_RS12535 rfaE2 2827872..2828363 (+) 492 WP_338770774.1 D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38073.65 Da        Isoelectric Point: 6.7833

>NTDB_id=943400 V6Z91_RS12500 WP_338770762.1 2822431..2823474(-) (pilT) [Massilia sp. METH4]
MDISDLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRLNVDPLEHKEVHRMIYDIMNDSQRKAYEEMLEVDFSFAIPGLAR
FRVNAYNQDRGASAVLRTIPSKVLTLEDLNAPRIFADFALKPRGLVLVTGPTGSGKSTTLAAMVNHLNEHEYGHILTIED
PIEFVHESKKCLVNQREVGPHTLSFSNALRSALREDPDAILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGAGRVAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGSNAGMQTLDQN
LTDLVRRNVISAATARSAAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=943400 V6Z91_RS12500 WP_338770762.1 2822431..2823474(-) (pilT) [Massilia sp. METH4]
ATGGACATCTCCGATCTGCTCGCATTCTCCGTGAAGAACAAGGCTTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCCAT
GATCCGCGTGCACGGCGATGTCCGCCGGCTGAACGTGGACCCGCTGGAGCACAAGGAAGTGCACCGCATGATCTATGACA
TCATGAACGACTCCCAGCGCAAGGCCTACGAGGAGATGCTGGAGGTCGATTTCAGCTTCGCGATTCCCGGCCTGGCGCGC
TTCCGCGTCAATGCCTACAACCAGGACCGCGGCGCATCCGCCGTGCTGCGCACCATCCCATCGAAAGTGCTCACGCTCGA
AGACTTGAACGCACCAAGGATCTTCGCCGATTTCGCGCTCAAGCCGCGCGGCCTGGTGCTCGTCACCGGCCCGACCGGTT
CCGGCAAGTCGACGACGCTGGCGGCCATGGTGAACCACCTGAACGAGCACGAATACGGCCACATCCTCACGATCGAGGAC
CCGATCGAATTCGTGCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAAGTGGGGCCGCACACCCTGTCGTTCAGCAA
TGCCCTGCGCTCGGCGCTGCGCGAAGACCCGGACGCGATCCTGGTCGGCGAGCTGCGCGACCTGGAAACGATCCGCCTGG
CATTGACCGCCGCCGAGACGGGCCACCTGGTGTTCGGCACGCTGCACACGTCGTCCGCCGCGAAGACCATCGACCGCATC
GTCGACGTGTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCGATGCTCTCGGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTCCTGAAGACGAAGGATGGCGCCGGCCGCGTGGCCGCGCACGAGATCATGGTGGGCACGCCGGCGATCCGCAACCTGA
TCCGCGAGGCGAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCAGCAACGCCGGCATGCAGACCCTGGACCAGAAC
CTGACGGACCTGGTGCGCCGCAACGTGATCTCGGCCGCCACCGCCCGCTCGGCCGCCAAGATCCCTGAAAACTTCCCCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.118

0.677
  pilT Vibrio cholerae strain A1552

69.733

97.118

0.677
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401
  pilU Vibrio cholerae strain A1552

41.003

97.695

0.401