Detailed information NTG0943399

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V6Z91_RS12495
• Genome accession: NZ_CP146614
• Coordinates: 2821277..2822413 (-)
• Length: 378 a.a.
• NCBI ID: WP_338770760.1
• Uniprot ID: -
• Organism: Massilia sp. METH4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2816277..2827413

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V6Z91_RS12485	-	2818050..2820536 (-)	2487	WP_338770756.1	PAS domain S-box protein	-
V6Z91_RS12490	-	2820784..2821269 (-)	486	WP_338770758.1	glutathione peroxidase	-
V6Z91_RS12495	pilU	2821277..2822413 (-)	1137	WP_338770760.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V6Z91_RS12500	pilT	2822431..2823474 (-)	1044	WP_338770762.1	type IV pilus twitching motility protein PilT	Machinery gene
V6Z91_RS12505	-	2823635..2824342 (+)	708	WP_338770763.1	YggS family pyridoxal phosphate-dependent enzyme	-
V6Z91_RS12510	proC	2824357..2825184 (+)	828	WP_338770764.1	pyrroline-5-carboxylate reductase	-
V6Z91_RS12515	-	2825184..2825405 (+)	222	WP_338770766.1	hypothetical protein	-
V6Z91_RS12520	-	2825454..2825819 (-)	366	WP_338770768.1	DUF883 domain-containing protein	-
V6Z91_RS12525	ubiA	2825923..2826798 (-)	876	WP_338770770.1	4-hydroxybenzoate octaprenyltransferase	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 41729.20 Da
• Isoelectric Point: 6.5794

>NTDB_id=943399 V6Z91_RS12495 WP_338770760.1 2821277..2822413(-) (pilU) [Massilia sp. METH4]
MERDQASKFMFDLLRLMVSKKGSDLFVTAGFPPAMKIDGKLTPVSAQPLTAAHTADLARAIMNDKQAATFEQTKEANFAI
SPGDLGRFRVSAFVQMGAAGMVLRVINSKIPDLDELALPPVLKEVVMAKRGLVIMVGATGSGKSTTLAAMVGYRNEHSHG
HIITIEDPVEFVHPHRNCIVTQREVGVDTEDWEVALKNTLRQAPDVIQIGEIRDRETMDHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLVPLKDAKGRAVAIEILLNSPLISDLIFQGKVHEIKELMKKSRELG
MQTFDQSLFDLYEADKISYEDALRNADSVNDLRLAIKLEGKAAKNRDLSAGTEHLGLI

Nucleotide

• Length: 1137 bp

>NTDB_id=943399 V6Z91_RS12495 WP_338770760.1 2821277..2822413(-) (pilU) [Massilia sp. METH4]
ATGGAACGCGACCAGGCCAGTAAATTCATGTTCGACCTGCTGCGCCTGATGGTGAGCAAGAAGGGGTCGGACCTGTTCGT
CACGGCGGGCTTTCCGCCGGCGATGAAGATCGACGGCAAGCTGACGCCCGTCTCCGCCCAGCCGTTGACGGCCGCGCACA
CGGCCGACCTGGCCCGCGCGATCATGAACGACAAGCAGGCCGCCACCTTCGAGCAGACGAAGGAGGCGAACTTCGCCATC
AGCCCCGGCGACCTGGGGCGCTTTCGCGTGTCCGCCTTCGTGCAGATGGGCGCCGCGGGCATGGTGCTGCGGGTCATCAA
CAGCAAGATCCCCGACCTGGACGAGCTGGCCCTGCCCCCGGTGCTGAAGGAGGTCGTGATGGCCAAGCGCGGCCTCGTGA
TCATGGTGGGCGCCACCGGCTCCGGCAAGTCGACCACGCTCGCCGCCATGGTGGGCTACCGCAATGAACACAGCCACGGC
CACATCATCACCATCGAAGACCCGGTGGAATTCGTGCACCCGCACCGCAACTGCATCGTCACGCAGCGCGAGGTGGGCGT
CGATACCGAGGATTGGGAAGTGGCGCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCGAAACGATGGACCACGCGATCGCCTTCGCCGAGACGGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGCCGCCAGCAACTGCTGATGGACCTGTCGCTGAACCT
GAAGGGCATGATCTCGCAGCGCCTGGTGCCGCTCAAGGATGCCAAGGGCCGCGCGGTGGCGATCGAGATCCTGCTCAATT
CCCCGTTGATCTCCGACCTGATCTTCCAGGGCAAGGTGCACGAGATCAAGGAGCTCATGAAGAAGTCGCGCGAATTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTGTACGAGGCCGACAAGATCAGTTACGAGGATGCGCTGCGCAATGCCGA
TTCGGTCAACGACCTGCGCCTGGCCATCAAGCTCGAAGGCAAGGCCGCGAAGAACCGCGACCTGTCGGCCGGCACCGAAC
ACCTGGGCCTGATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (46-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.74	96.561	0.606
pilU	Acinetobacter baylyi ADP1	60.504	94.444	0.571
pilU	Vibrio cholerae strain A1552	59.531	90.212	0.537
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.881	88.624	0.389
pilT	Pseudomonas aeruginosa PAK	42.687	88.624	0.378
pilT	Legionella pneumophila strain ERS1305867	41.692	87.566	0.365
pilT	Legionella pneumophila strain Lp02	41.692	87.566	0.365
pilT	Acinetobacter nosocomialis M2	41.39	87.566	0.362
pilT	Acinetobacter baumannii D1279779	41.39	87.566	0.362
pilT	Acinetobacter baumannii strain A118	41.39	87.566	0.362