Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSGK_RS25110 Genome accession   NZ_CP146285
Coordinates   5561111..5562145 (+) Length   344 a.a.
NCBI ID   WP_021218361.1    Uniprot ID   A0AAU7Y4C8
Organism   Pseudomonas solani strain GK13     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5556111..5567145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSGK_RS25085 (PSGK_24945) - 5557243..5557539 (-) 297 WP_021218356.1 DUF167 domain-containing protein -
  PSGK_RS25090 (PSGK_24950) - 5557539..5558132 (-) 594 WP_265170282.1 YggT family protein -
  PSGK_RS25095 (PSGK_24955) - 5558295..5559509 (-) 1215 WP_374979600.1 hypothetical protein -
  PSGK_RS25100 (PSGK_24960) proC 5559515..5560333 (-) 819 WP_021218359.1 pyrroline-5-carboxylate reductase -
  PSGK_RS25105 (PSGK_24965) - 5560347..5561042 (-) 696 WP_374979601.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSGK_RS25110 (PSGK_24970) pilT 5561111..5562145 (+) 1035 WP_021218361.1 type IV pilus twitching motility protein PilT Machinery gene
  PSGK_RS25115 (PSGK_24975) pilU 5562275..5563420 (+) 1146 WP_021218362.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSGK_RS25120 (PSGK_24980) - 5563353..5564846 (-) 1494 WP_374979602.1 UdgX family uracil-DNA binding protein -
  PSGK_RS25125 (PSGK_24985) - 5564846..5566060 (-) 1215 WP_265170287.1 putative DNA modification/repair radical SAM protein -
  PSGK_RS25130 (PSGK_24990) - 5566162..5566557 (+) 396 WP_021218365.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37817.52 Da        Isoelectric Point: 6.8359

>NTDB_id=942501 PSGK_RS25110 WP_021218361.1 5561111..5562145(+) (pilT) [Pseudomonas solani strain GK13]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGDIFKKISDVPRGLVLVTGPTGSGKSTTLAAMLDYLNGTKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGGLGMQTLDSCL
KGLVAKGLVSRESAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=942501 PSGK_RS25110 WP_021218361.1 5561111..5562145(+) (pilT) [Pseudomonas solani strain GK13]
ATGGATATTACTGAGCTGCTTGCCTTCAGCGCGAAGCAGGGCGCGTCGGACCTGCATCTCTCTGCTGGCCTGCCGCCGAT
GATCCGCGTCGACGGTGACGTGCGCCGCATCAACCTGCCGGCCATGGACCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTACGAGGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTGCCCGGCGTAGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCCGTGTTCCGTACCATTCCCTCGAAGGTACTGAGCATGGA
AGACCTGGGCATGGGCGATATCTTCAAGAAGATCTCCGACGTGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACCGGCT
CGGGCAAGTCCACCACCTTGGCGGCGATGCTCGACTACCTCAACGGCACCAAGTACCACCACATCCTCACGGTCGAAGAC
CCCATCGAATTCGTTCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCTCGGCTTCAACGA
AGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATCCTGGTGGGTGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAGGAAAAGTCCATGGTCCGTTCGATGCTCTCCGAATCCCTGCAGTCGGTCATTTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGCGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCGCCATCCAGACCGGCGGCGGCCTCGGCATGCAGACCCTGGACAGCTGCCTC
AAGGGGCTGGTGGCCAAGGGCCTGGTGAGCCGCGAAAGCGCGCGCGAAAAGGCCAAGAGCCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

94.186

100

0.942
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter baumannii D1279779

85.756

100

0.858
  pilT Acinetobacter baumannii strain A118

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

85.465

100

0.855
  pilT Acinetobacter baylyi ADP1

83.721

100

0.837
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

39.42

100

0.395
  pilU Vibrio cholerae strain A1552

39.42

100

0.395