Detailed information NTG0942502

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSGK_RS25115
• Genome accession: NZ_CP146285
• Coordinates: 5562275..5563420 (+)
• Length: 381 a.a.
• NCBI ID: WP_021218362.1
• Uniprot ID: A0AAU7Y6D2
• Organism: Pseudomonas solani strain GK13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5557275..5568420

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSGK_RS25090 (PSGK_24950)	-	5557539..5558132 (-)	594	WP_265170282.1	YggT family protein	-
PSGK_RS25095 (PSGK_24955)	-	5558295..5559509 (-)	1215	WP_374979600.1	hypothetical protein	-
PSGK_RS25100 (PSGK_24960)	proC	5559515..5560333 (-)	819	WP_021218359.1	pyrroline-5-carboxylate reductase	-
PSGK_RS25105 (PSGK_24965)	-	5560347..5561042 (-)	696	WP_374979601.1	YggS family pyridoxal phosphate-dependent enzyme	-
PSGK_RS25110 (PSGK_24970)	pilT	5561111..5562145 (+)	1035	WP_021218361.1	type IV pilus twitching motility protein PilT	Machinery gene
PSGK_RS25115 (PSGK_24975)	pilU	5562275..5563420 (+)	1146	WP_021218362.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSGK_RS25120 (PSGK_24980)	-	5563353..5564846 (-)	1494	WP_374979602.1	UdgX family uracil-DNA binding protein	-
PSGK_RS25125 (PSGK_24985)	-	5564846..5566060 (-)	1215	WP_265170287.1	putative DNA modification/repair radical SAM protein	-
PSGK_RS25130 (PSGK_24990)	-	5566162..5566557 (+)	396	WP_021218365.1	TM2 domain-containing protein	-
PSGK_RS25135 (PSGK_24995)	-	5566617..5567888 (-)	1272	WP_374979603.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42463.75 Da
• Isoelectric Point: 6.5931

>NTDB_id=942502 PSGK_RS25115 WP_021218362.1 5562275..5563420(+) (pilU) [Pseudomonas solani strain GK13]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEQTRETVLAVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYTLYSQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMSGGLSLEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=942502 PSGK_RS25115 WP_021218362.1 5562275..5563420(+) (pilU) [Pseudomonas solani strain GK13]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAGAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTCCCCCCGTC
GATGAAGGTCAACGGCAAGATCATGCCCGTGACCAAGAACGCCATGTCCCCGGAGCAGACCCGTGAAACCGTTCTCGCGG
TGATGAACGAGCAGCAGCGCCGTGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGGGGCATCGGCCGT
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCCTGGA
TGACCTCAAGCTGCCGGAAATCCTCAAGAAGCTGGCACTGACCAAGCGCGGCCTGGTGCTCTTCGTCGGCGCCACCGGTA
CCGGCAAGTCCACTTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTACATCCACCAGCACCAGAGCTGCATCGTCACCCAGCGTGAAGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGTGAGGTGCGGACCCGCGAAACCATGGACCATG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCTGCCGACCGTCAGCAGCAGGTGTGGATGGACCTGTCCCTCAACCTCAAGGCCATCGTCGCTCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGTCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACACCCTCTACTCCCAGGGCGAAATCACCTACGAAGACGCGCTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCCGAAACCGACGGCGACCACCTCACGAGCATGAGCGGTGGCCTGTCGCTGGAAGTGTCCGAGG
AAGACCCGGGCCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.339	100	0.913
pilU	Acinetobacter baylyi ADP1	64.426	93.701	0.604
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.155	88.189	0.381