Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V6657_RS13590 Genome accession   NZ_CP146091
Coordinates   2812064..2813107 (-) Length   347 a.a.
NCBI ID   WP_039600020.1    Uniprot ID   A0A0B1XZR1
Organism   Ralstonia sp. RRA.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2807064..2818107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6657_RS13560 - 2807349..2808080 (+) 732 WP_048936087.1 LrgB family protein -
  V6657_RS13565 - 2808251..2808646 (+) 396 WP_027680594.1 VOC family protein -
  V6657_RS13570 - 2808709..2809188 (+) 480 WP_048936086.1 cupin domain-containing protein -
  V6657_RS13575 - 2809255..2809953 (+) 699 WP_048936085.1 YafY family protein -
  V6657_RS13580 - 2809950..2810453 (-) 504 WP_048936084.1 glutathione peroxidase -
  V6657_RS13585 pilU 2810858..2811997 (-) 1140 WP_048936083.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V6657_RS13590 pilT 2812064..2813107 (-) 1044 WP_039600020.1 type IV pilus twitching motility protein PilT Machinery gene
  V6657_RS13595 - 2813230..2813952 (+) 723 WP_048936082.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6657_RS13600 proC 2813990..2814814 (+) 825 WP_048936103.1 pyrroline-5-carboxylate reductase -
  V6657_RS13605 ubiA 2815382..2816257 (-) 876 WP_048933001.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38207.82 Da        Isoelectric Point: 6.8307

>NTDB_id=941666 V6657_RS13590 WP_039600020.1 2812064..2813107(-) (pilT) [Ralstonia sp. RRA.1]
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHSMVYDIMSDGQRKVYEENLEVDFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLTLDDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKEMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMIATPAIRHLIRENKISQMYSMMQTSSGMGMQTLDQC
LSELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=941666 V6657_RS13590 WP_039600020.1 2812064..2813107(-) (pilT) [Ralstonia sp. RRA.1]
ATGGACATCGCGCAGCTTCTGGCGTTTTCCGCCAAGAACAAAGCGTCGGACTTGCACTTGTCGGCCGGTTTGCCGCCGAT
GATCCGGATCCACGGCGACATGCGCCGGATCAACGTGCCACCGCTCACGCACCAAGACGTGCACTCCATGGTGTACGACA
TCATGAGCGACGGGCAGCGCAAGGTCTACGAAGAAAACCTTGAAGTCGACTTCTCGTTTGAGATCCCGGGGCTCTCGCGT
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCTTCGGCTGTGTTCCGGACGATTCCGTCGAAGGTGCTCACGCTGGA
CGACCTGAAGGCGCCGGCCGTGTTTGCCGACCTGGCGATGAAGCCGCGTGGCTTGGTGCTTGTCACGGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTGAACCACCGCAACGAAAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGGCCGCATACGCACTCGTTCGCCAA
TGCGTTGAAGTCGGCACTGCGTGAAGATCCGGACGTGGTGCTGGTCGGCGAATTGCGTGATCTGGAAACCATTCGCCTGG
CACTCACCGCGGCGGAAACCGGCCACTTGGTGTTCGGTACGCTGCACACCAGCTCCGCTGCGAAGACCATCGACCGGGTC
GTCGACGTGTTCCCGTCGGACGAGAAAGAGATGGTCCGGACCATGCTTTCCGAATCGCTGGAAGCGGTGATCTCGCAGAC
GCTACTCAAGACGCGCGACGGCTCGGGCCGGATTGCGGCGCACGAGATCATGATCGCCACGCCGGCCATCCGCCACTTGA
TCCGCGAGAACAAGATCTCGCAGATGTATTCGATGATGCAGACCAGCAGCGGCATGGGCATGCAGACGCTCGACCAGTGC
CTGTCGGAGCTCATCAAGCGCTCGGCGATCAACTACGCAGACGCACGCGCCATCGCCAAGAACCCGGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B1XZR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.304

99.424

0.709
  pilT Acinetobacter baumannii D1279779

71.304

99.424

0.709
  pilT Acinetobacter baumannii strain A118

71.304

99.424

0.709
  pilT Pseudomonas aeruginosa PAK

70.435

99.424

0.7
  pilT Pseudomonas stutzeri DSM 10701

70.145

99.424

0.697
  pilT Acinetobacter baylyi ADP1

69.275

99.424

0.689
  pilT Neisseria gonorrhoeae MS11

68.497

99.712

0.683
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Legionella pneumophila strain Lp02

66.667

99.424

0.663
  pilT Legionella pneumophila strain ERS1305867

66.667

99.424

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.469

97.118

0.646
  pilT Vibrio cholerae strain A1552

66.469

97.118

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.578

99.712

0.504
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

41.194

96.542

0.398