Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V6657_RS13585 Genome accession   NZ_CP146091
Coordinates   2810858..2811997 (-) Length   379 a.a.
NCBI ID   WP_048936083.1    Uniprot ID   -
Organism   Ralstonia sp. RRA.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2805858..2816997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6657_RS13555 - 2806975..2807352 (+) 378 WP_021195925.1 CidA/LrgA family protein -
  V6657_RS13560 - 2807349..2808080 (+) 732 WP_048936087.1 LrgB family protein -
  V6657_RS13565 - 2808251..2808646 (+) 396 WP_027680594.1 VOC family protein -
  V6657_RS13570 - 2808709..2809188 (+) 480 WP_048936086.1 cupin domain-containing protein -
  V6657_RS13575 - 2809255..2809953 (+) 699 WP_048936085.1 YafY family protein -
  V6657_RS13580 - 2809950..2810453 (-) 504 WP_048936084.1 glutathione peroxidase -
  V6657_RS13585 pilU 2810858..2811997 (-) 1140 WP_048936083.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V6657_RS13590 pilT 2812064..2813107 (-) 1044 WP_039600020.1 type IV pilus twitching motility protein PilT Machinery gene
  V6657_RS13595 - 2813230..2813952 (+) 723 WP_048936082.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6657_RS13600 proC 2813990..2814814 (+) 825 WP_048936103.1 pyrroline-5-carboxylate reductase -
  V6657_RS13605 ubiA 2815382..2816257 (-) 876 WP_048933001.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42107.45 Da        Isoelectric Point: 6.6638

>NTDB_id=941665 V6657_RS13585 WP_048936083.1 2810858..2811997(-) (pilU) [Ralstonia sp. RRA.1]
MLDREAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLSAVQAIGLVKSIMNEKQLREYEDSLECNFA
ITAPGAGRFRVSAFIQQGRAGMVLRTINTKIPSLGELDLPPILNEIVMSKRGLVIVVGATGSGKSTSLAAMVGYRNANSY
GHIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRQGKKGRVPAVEIMLATPLVQDLIFKGEVHELKEVMKKSREQ
GMVSFDQALFDLYEEGKISYEDALRNADSLNDLRLQIKLHSKRGGQTDLSAGTEHLNVV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=941665 V6657_RS13585 WP_048936083.1 2810858..2811997(-) (pilU) [Ralstonia sp. RRA.1]
ATGCTGGACCGCGAAGCCGCCACCAAATACATCCACGAACTCTTGCAGTTGATGGTGAACAGCCGTGGCTCGGACTTGTT
CATCACCGCAGAATTTCCGCCCGCCATCAAGGTGGACGGCAAGGTCACGCCGATCTCGCAGCAGCCGCTGAGTGCGGTGC
AGGCCATCGGTCTGGTCAAGTCGATCATGAACGAGAAGCAACTGCGCGAGTACGAAGACAGCTTGGAGTGCAACTTCGCC
ATCACGGCGCCGGGGGCAGGGCGCTTCCGTGTGTCGGCGTTCATCCAGCAGGGCCGCGCTGGCATGGTGCTGCGGACCAT
CAACACGAAGATCCCCTCGTTGGGCGAGCTGGACCTGCCGCCGATCTTGAACGAGATCGTGATGAGCAAGCGCGGGCTGG
TGATCGTGGTGGGTGCCACGGGCTCGGGCAAGTCGACCTCGCTGGCGGCCATGGTGGGCTACCGCAACGCCAATTCGTAC
GGCCACATCATCACCATTGAAGACCCGGTGGAATATGTGCACGCACACCAGAACTGCGTGGTCACGCAGCGCGAGGTGGG
GGTGGATACCGAGTCGTGGCACGTGGCGCTGAAGAACACACTGCGTCAGGCGCCAGACGTGATCCTGATCGGCGAAATTC
GTGACCGCGACACGATGGAGTACGCCATCCAGTACGCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGAAGCGTCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGCGCGCGATGATCGCGCAGCGGCTGCTGCCGCGCCAGGGCAAAAAAGGCCGCGTGCCGGCGGTGGAAATCATGCTGG
CGACACCGCTGGTGCAGGACCTGATCTTCAAGGGCGAGGTGCACGAGCTGAAAGAGGTCATGAAGAAATCCCGCGAGCAA
GGGATGGTTTCGTTCGACCAGGCGCTCTTCGATCTGTATGAAGAAGGCAAGATCAGCTACGAAGATGCGCTGCGCAATGC
CGACTCGCTGAACGATCTGCGCCTGCAGATCAAGCTGCATAGCAAGCGTGGTGGGCAGACGGATCTGTCGGCGGGGACCG
AGCATCTGAACGTGGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.326

95.515

0.586
  pilU Acinetobacter baylyi ADP1

56.286

92.348

0.52
  pilU Vibrio cholerae strain A1552

53.39

93.404

0.499
  pilT Vibrio cholerae strain A1552

44.277

87.599

0.388
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.277

87.599

0.388
  pilT Acinetobacter nosocomialis M2

44.144

87.863

0.388
  pilT Acinetobacter baumannii strain A118

44.144

87.863

0.388
  pilT Acinetobacter baumannii D1279779

44.144

87.863

0.388
  pilT Acinetobacter baylyi ADP1

44.144

87.863

0.388
  pilT Legionella pneumophila strain Lp02

43.844

87.863

0.385
  pilT Legionella pneumophila strain ERS1305867

43.844

87.863

0.385
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

43.027

88.918

0.383
  pilT Pseudomonas stutzeri DSM 10701

42.433

88.918

0.377
  pilT Neisseria meningitidis 8013

40.533

89.182

0.361