Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V5M36_RS12185 Genome accession   NZ_CP145906
Coordinates   2702477..2703514 (+) Length   345 a.a.
NCBI ID   WP_338559398.1    Uniprot ID   -
Organism   Acinetobacter sp. KS-LM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2697477..2708514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5M36_RS12175 (V5M36_12175) - 2697881..2701477 (+) 3597 WP_338559396.1 AAA family ATPase -
  V5M36_RS12180 (V5M36_12180) - 2701669..2702355 (-) 687 WP_338559397.1 YggS family pyridoxal phosphate-dependent enzyme -
  V5M36_RS12185 (V5M36_12185) pilT 2702477..2703514 (+) 1038 WP_338559398.1 type IV pilus twitching motility protein PilT Machinery gene
  V5M36_RS12190 (V5M36_12190) pilU 2703545..2704663 (+) 1119 WP_338559399.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V5M36_RS12195 (V5M36_12195) fur 2704771..2705208 (-) 438 WP_338559401.1 ferric iron uptake transcriptional regulator -
  V5M36_RS12200 (V5M36_12200) bamE 2705321..2705719 (+) 399 WP_338559403.1 outer membrane protein assembly factor BamE -
  V5M36_RS12205 (V5M36_12205) - 2705766..2706083 (-) 318 WP_338564199.1 RnfH family protein -
  V5M36_RS12210 (V5M36_12210) - 2706083..2707168 (-) 1086 WP_338559405.1 hypothetical protein -
  V5M36_RS12215 (V5M36_12215) - 2707352..2708200 (-) 849 WP_338559407.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38369.99 Da        Isoelectric Point: 6.3485

>NTDB_id=941069 V5M36_RS12185 WP_338559398.1 2702477..2703514(+) (pilT) [Acinetobacter sp. KS-LM10]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHNEVHRLIYDIMNDKQRRDYEEKLETDFSFEVPGLAR
FRVNAFTQNRGAGAVFRTIPSKVLSMEDLGMGQIFKDICEYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHESKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKTGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSSIQTGSNYGMTTLDQSL
KNLVAKGTISAQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=941069 V5M36_RS12185 WP_338559398.1 2702477..2703514(+) (pilT) [Acinetobacter sp. KS-LM10]
ATGGACATTACAGAATTACTGGCTTTTTCAGTCAAAAATGGCGCATCTGATTTACACCTATCTGCGGGTATGCCGCCCAT
GATCCGAGTGGATGGTGAGGTTCGTAGAATCAATCTTCCAGCACTCGAACATAATGAAGTCCATCGTTTAATTTATGACA
TCATGAATGATAAACAGCGACGTGATTATGAAGAAAAATTAGAAACTGACTTTTCATTTGAAGTGCCCGGTCTTGCTCGC
TTCCGTGTCAATGCTTTTACTCAAAATCGCGGTGCAGGCGCTGTTTTTCGTACGATTCCATCGAAAGTTCTCTCGATGGA
AGATCTGGGTATGGGACAAATTTTTAAAGACATTTGTGAATATCCTCGCGGAATCGTCTTGGTCACGGGTCCAACTGGTT
CAGGTAAATCTACAACACTTGCAGCGATGATGGATTATATTAATGAAAATCGCTATGACCATATTTTGACTGTTGAAGAT
CCTATCGAGTTTGTTCATGAATCTAAAAAGTGTCTGATTAATCAGCGTGAAGTCCATCGTGATACGCATGGTTTTAATGA
AGCTTTACGCTCTGCTTTACGTGAAGATCCAGACATTATTTTGGTCGGTGAAATGCGTGACTTAGAAACCATTCGCTTAG
CCCTTACCGCAGCGGAAACAGGCCACTTAGTCTTTGGCACCCTACACACAACCTCTGCAGCAAAAACCATTGACCGTATT
ATTGATGTCTTCCCTGCAGAAGAAAAAGACATGGTTCGAGCGATGCTATCGGAATCACTTCAAGCTGTTATTTCACAAAC
CCTGCTTAAAAAAACGGGCGGTGGTCGTGTAGCTGCTCATGAAATTATGATTGGCATTCCCGCTATCCGAAATCTCATTC
GTGAAAATAAAGTTGCACAAATGTACTCATCCATTCAAACAGGCTCCAACTATGGCATGACTACACTGGATCAAAGTTTA
AAAAACTTGGTCGCTAAAGGAACTATCAGTGCACAAGTTGCAAGAACAGCAGCGAAGCAACCTGAGTCTTTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

93.913

100

0.939
  pilT Acinetobacter baumannii strain A118

93.913

100

0.939
  pilT Acinetobacter nosocomialis M2

93.623

100

0.936
  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.084

96.232

0.684
  pilT Vibrio cholerae strain A1552

71.084

96.232

0.684
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Vibrio cholerae strain A1552

41.003

98.261

0.403
  pilU Acinetobacter baylyi ADP1

39.595

100

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.101

0.391