Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V5M36_RS12190 Genome accession   NZ_CP145906
Coordinates   2703545..2704663 (+) Length   372 a.a.
NCBI ID   WP_338559399.1    Uniprot ID   -
Organism   Acinetobacter sp. KS-LM10     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2698545..2709663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5M36_RS12180 (V5M36_12180) - 2701669..2702355 (-) 687 WP_338559397.1 YggS family pyridoxal phosphate-dependent enzyme -
  V5M36_RS12185 (V5M36_12185) pilT 2702477..2703514 (+) 1038 WP_338559398.1 type IV pilus twitching motility protein PilT Machinery gene
  V5M36_RS12190 (V5M36_12190) pilU 2703545..2704663 (+) 1119 WP_338559399.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V5M36_RS12195 (V5M36_12195) fur 2704771..2705208 (-) 438 WP_338559401.1 ferric iron uptake transcriptional regulator -
  V5M36_RS12200 (V5M36_12200) bamE 2705321..2705719 (+) 399 WP_338559403.1 outer membrane protein assembly factor BamE -
  V5M36_RS12205 (V5M36_12205) - 2705766..2706083 (-) 318 WP_338564199.1 RnfH family protein -
  V5M36_RS12210 (V5M36_12210) - 2706083..2707168 (-) 1086 WP_338559405.1 hypothetical protein -
  V5M36_RS12215 (V5M36_12215) - 2707352..2708200 (-) 849 WP_338559407.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41973.13 Da        Isoelectric Point: 6.6414

>NTDB_id=941070 V5M36_RS12190 WP_338559399.1 2703545..2704663(+) (pilU) [Acinetobacter sp. KS-LM10]
MDFNDLLEMMITHKASDLFISADVEPSMKINGEINPVSKTKLTSEIVAKLIQSIMTEKQKQEFKNTRECNFAISNKDKTA
RFRVSAFQQREQPGMVLRRIETEIPTLEELRLPPILKELAMSKRGIIIFVGGTGTGKSTSLAAVIGYRNQNSKGHIITIE
DPIEFIHQHAGCIITQREVGIDTDSYEVALKNTLRQAPDVILIGEIRSRETMDYALAFAETGHLVLATLHANNANQAIDR
IIHFFDTDRHSQVFMDLSLNLKAIIAQQLVVDYSGSSRHAAIEVLINSPLIADLIRKGEIHEIKELMQRSRELGMQTFDQ
ALFDLYKARKISYKDALKHADSANNLRLQIKLSEENSDHLNNAAMKLSFDNE

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=941070 V5M36_RS12190 WP_338559399.1 2703545..2704663(+) (pilU) [Acinetobacter sp. KS-LM10]
ATGGATTTTAATGATCTGCTTGAAATGATGATCACACATAAAGCATCTGACTTATTCATCAGTGCTGATGTTGAACCTTC
TATGAAAATAAATGGAGAAATAAATCCTGTTTCTAAAACAAAATTAACAAGTGAAATCGTCGCTAAACTCATTCAGTCAA
TAATGACCGAAAAACAAAAACAAGAATTTAAGAACACCCGAGAATGCAACTTTGCCATTTCTAATAAAGATAAAACCGCA
AGATTTCGAGTCAGTGCGTTTCAACAACGTGAACAACCAGGCATGGTTCTAAGACGGATTGAAACTGAAATTCCAACGTT
GGAAGAACTCAGACTCCCGCCTATTCTTAAAGAACTTGCTATGTCTAAGCGCGGCATTATTATCTTTGTCGGTGGAACGG
GAACAGGTAAATCAACATCCCTCGCAGCGGTCATTGGTTATCGGAATCAAAATTCCAAAGGCCATATTATTACCATTGAA
GATCCGATTGAGTTTATTCACCAACATGCGGGCTGTATTATTACCCAACGTGAAGTGGGTATAGACACAGACTCTTACGA
AGTTGCGCTGAAAAATACATTACGCCAAGCACCCGATGTGATTTTGATCGGTGAAATTCGCTCTCGGGAAACGATGGATT
ATGCATTGGCCTTTGCTGAAACAGGACATTTGGTGCTTGCCACCTTGCACGCGAACAACGCGAATCAGGCCATTGATCGG
ATTATTCACTTTTTTGATACTGACCGTCATAGCCAAGTGTTCATGGATTTATCTTTGAACTTAAAAGCTATTATTGCTCA
ACAATTGGTAGTCGATTATTCAGGAAGCTCTCGACATGCTGCCATTGAGGTTTTAATAAACTCTCCATTGATCGCAGACT
TGATTCGTAAAGGTGAGATTCATGAAATCAAAGAGCTCATGCAGCGATCTCGCGAGCTTGGTATGCAAACGTTTGATCAA
GCTTTATTTGATTTATATAAAGCCAGAAAAATCAGTTATAAAGATGCGTTAAAACATGCTGATTCAGCCAATAATCTTCG
TTTACAAATTAAATTATCTGAAGAAAATTCTGATCATTTAAACAATGCTGCTATGAAACTGAGTTTCGATAATGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

74.339

100

0.755
  pilU Pseudomonas stutzeri DSM 10701

66.577

99.731

0.664
  pilU Vibrio cholerae strain A1552

56.452

100

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.274

98.118

0.395
  pilT Legionella pneumophila strain Lp02

42.604

90.86

0.387
  pilT Legionella pneumophila strain ERS1305867

42.604

90.86

0.387
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.812

86.022

0.368
  pilT Vibrio cholerae strain A1552

42.812

86.022

0.368
  pilT Acinetobacter nosocomialis M2

40.476

90.323

0.366
  pilT Acinetobacter baumannii D1279779

40.476

90.323

0.366
  pilT Acinetobacter baumannii strain A118

40.476

90.323

0.366
  pilT Pseudomonas aeruginosa PAK

38.728

93.011

0.36
  pilT Neisseria meningitidis 8013

38.728

93.011

0.36
  pilT Neisseria gonorrhoeae MS11

38.728

93.011

0.36