Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V6W80_RS03330 Genome accession   NZ_CP145723
Coordinates   704783..705817 (+) Length   344 a.a.
NCBI ID   WP_338545838.1    Uniprot ID   -
Organism   Pseudomonas benzopyrenica strain MLY92     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 699783..710817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6W80_RS03310 (V6W80_03310) - 700454..702412 (-) 1959 WP_184292033.1 dynamin family protein -
  V6W80_RS03315 (V6W80_03315) - 702530..703123 (-) 594 WP_338545836.1 YggT family protein -
  V6W80_RS03320 (V6W80_03320) proC 703133..703951 (-) 819 WP_017640988.1 pyrroline-5-carboxylate reductase -
  V6W80_RS03325 (V6W80_03325) - 703961..704656 (-) 696 WP_338545837.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6W80_RS03330 (V6W80_03330) pilT 704783..705817 (+) 1035 WP_338545838.1 type IV pilus twitching motility protein PilT Machinery gene
  V6W80_RS03335 (V6W80_03335) pilU 705817..706959 (+) 1143 WP_017640986.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V6W80_RS03340 (V6W80_03340) - 706956..708404 (-) 1449 WP_064309242.1 UdgX family uracil-DNA binding protein -
  V6W80_RS03345 (V6W80_03345) - 708405..709616 (-) 1212 WP_017640984.1 putative DNA modification/repair radical SAM protein -
  V6W80_RS03350 (V6W80_03350) - 709749..710306 (-) 558 WP_017640983.1 C40 family peptidase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37888.68 Da        Isoelectric Point: 6.6635

>NTDB_id=939726 V6W80_RS03330 WP_338545838.1 704783..705817(+) (pilT) [Pseudomonas benzopyrenica strain MLY92]
MDITELLAFSARQGASDLHLSAGLPPMIRVDGDVRRINLPAFEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEELGMGEVFKKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGGLGMQTLDMCL
KALLAKGLISCDAAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=939726 V6W80_RS03330 WP_338545838.1 704783..705817(+) (pilT) [Pseudomonas benzopyrenica strain MLY92]
ATGGATATCACCGAATTGCTGGCCTTTTCCGCGCGGCAAGGCGCCTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCAACCTGCCGGCCTTCGAGCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTGGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAATCGCGGCGCCGGCGCGGTGTTCCGGACCATCCCTTCCAAGGTGCTGAGCATGGA
AGAGCTGGGCATGGGCGAGGTGTTCAAGAAGATCACCGACGTTCCGCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTCGCGGCCATGCTCGACTACCTCAACAACACCAAGTACCACCACATCCTCACCATCGAGGAC
CCCATCGAATTCGTCCACGAATCCAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTGGGCTTCTCCGA
GGCCCTGCGCTCGGCGCTGCGCGAGGACCCGGACATCATCCTGGTCGGCGAGCTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGGCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGATCGGGTG
GTGGACGTCTTTCCGGCGGAAGAGAAGTCCATGGTGCGTTCCATGCTGTCCGAATCCCTGCAGGCGGTGGTCTCCCAGAC
CCTGCTCAAGAAGGTCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGTACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCAATCCAGACCGGTGGCGGCCTGGGCATGCAGACCCTGGACATGTGCCTC
AAGGCGCTCCTGGCCAAGGGGCTGATCAGCTGCGACGCGGCCCGCGAGAAGGCCAAGACGCCGGAGAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.349

100

0.953
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter nosocomialis M2

82.849

100

0.828
  pilT Acinetobacter baylyi ADP1

82.558

100

0.826
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39