Detailed information NTG0939727

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V6W80_RS03335
• Genome accession: NZ_CP145723
• Coordinates: 705817..706959 (+)
• Length: 380 a.a.
• NCBI ID: WP_017640986.1
• Uniprot ID: A0A1H8VYP4
• Organism: Pseudomonas benzopyrenica strain MLY92
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 700817..711959

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V6W80_RS03315 (V6W80_03315)	-	702530..703123 (-)	594	WP_338545836.1	YggT family protein	-
V6W80_RS03320 (V6W80_03320)	proC	703133..703951 (-)	819	WP_017640988.1	pyrroline-5-carboxylate reductase	-
V6W80_RS03325 (V6W80_03325)	-	703961..704656 (-)	696	WP_338545837.1	YggS family pyridoxal phosphate-dependent enzyme	-
V6W80_RS03330 (V6W80_03330)	pilT	704783..705817 (+)	1035	WP_338545838.1	type IV pilus twitching motility protein PilT	Machinery gene
V6W80_RS03335 (V6W80_03335)	pilU	705817..706959 (+)	1143	WP_017640986.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V6W80_RS03340 (V6W80_03340)	-	706956..708404 (-)	1449	WP_064309242.1	UdgX family uracil-DNA binding protein	-
V6W80_RS03345 (V6W80_03345)	-	708405..709616 (-)	1212	WP_017640984.1	putative DNA modification/repair radical SAM protein	-
V6W80_RS03350 (V6W80_03350)	-	709749..710306 (-)	558	WP_017640983.1	C40 family peptidase	-
V6W80_RS03355 (V6W80_03355)	-	710741..711145 (+)	405	WP_017640982.1	TM2 domain-containing protein	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42412.72 Da
• Isoelectric Point: 7.3062

>NTDB_id=939727 V6W80_RS03335 WP_017640986.1 705817..706959(+) (pilU) [Pseudomonas benzopyrenica strain MLY92]
MDFDRFLQLMVDKGASDLFITTGVPPSMKINGRMTPVTKDSLTPDQCRAIVLGVMNDEQRQEFQQKRECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIEVNIPTVEQLRLPEIIKKLAMTKRGLVIFVGATGTGKSTSLAAMIGHRNRNSAGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEIRSRDTMEHALAFAETGHLCLATLHANNANQALDRI
IHFFPADRHSQTWMDLSLNLKGIVAQQLVPTTDGQGRRAVIEVLLNTPLAADLIRKGEVHELKALMKKSGELGMQTFDQS
LYGLYRDGEISYEAALAHADSANDLRLMIKLGSETDADHLNQLTRNLSVDMGDDPPKRFR

Nucleotide

• Length: 1143 bp

>NTDB_id=939727 V6W80_RS03335 WP_017640986.1 705817..706959(+) (pilU) [Pseudomonas benzopyrenica strain MLY92]
ATGGATTTCGACAGGTTCCTGCAACTGATGGTGGACAAGGGGGCGTCCGACCTCTTCATCACCACCGGCGTGCCTCCCTC
GATGAAGATCAACGGGCGCATGACGCCGGTCACCAAGGATTCGCTCACGCCGGATCAGTGCCGAGCCATCGTGCTGGGGG
TGATGAACGACGAGCAGCGGCAGGAATTCCAGCAGAAGCGCGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGTCGC
TTCCGGGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGGTCAACATTCCCACGGTCGA
GCAGCTGCGCCTGCCGGAGATCATCAAGAAGCTGGCCATGACCAAGCGCGGGCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCCATGATCGGCCATCGCAACAGGAACTCGGCGGGACACATCATTTCCATCGAGGAC
CCCATCGAATACATCCACCAGCACCAGGGGTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCGAATCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCATGATCGGCGAGATCCGCAGCCGGGACACCATGGAGCACG
CCCTGGCCTTCGCCGAGACCGGTCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCCATTTCTTCCCGGCCGATCGGCACAGCCAGACCTGGATGGACCTGTCGCTCAATCTCAAGGGCATCGTCGCCCAGCA
ACTGGTGCCCACCACCGATGGCCAGGGCCGTCGTGCCGTGATCGAGGTGCTGCTCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAACTCAAGGCGCTGATGAAGAAGTCCGGCGAACTGGGCATGCAGACCTTCGACCAGTCG
CTCTATGGTCTCTATCGCGACGGCGAGATCAGCTACGAAGCGGCCTTGGCCCACGCCGATTCCGCCAACGACCTGCGGCT
GATGATCAAGCTGGGGTCGGAAACGGACGCCGATCACCTCAATCAGCTCACCCGCAACCTCTCGGTGGACATGGGCGACG
ATCCGCCCAAGCGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: A0A1H8VYP4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	77.165	100	0.774
pilU	Acinetobacter baylyi ADP1	62.766	98.947	0.621
pilU	Vibrio cholerae strain A1552	56.873	97.632	0.555
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.888	89.211	0.374