Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V6N89_RS16550 Genome accession   NZ_CP145482
Coordinates   3436566..3437609 (-) Length   347 a.a.
NCBI ID   WP_338518549.1    Uniprot ID   -
Organism   Alteromonas gracilis strain J4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3431566..3442609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6N89_RS16515 - 3431613..3431792 (+) 180 WP_346992971.1 hypothetical protein -
  V6N89_RS16520 - 3432186..3432914 (+) 729 WP_338518546.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  V6N89_RS16525 gshB 3433071..3434025 (+) 955 Protein_3232 glutathione synthase -
  V6N89_RS16530 - 3434087..3434653 (+) 567 WP_346992972.1 YqgE/AlgH family protein -
  V6N89_RS16535 ruvX 3434646..3435077 (+) 432 WP_338518547.1 Holliday junction resolvase RuvX -
  V6N89_RS16540 - 3435084..3435413 (-) 330 WP_338518548.1 DUF2007 domain-containing protein -
  V6N89_RS16545 pilU 3435444..3436556 (-) 1113 WP_346992973.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V6N89_RS16550 pilT 3436566..3437609 (-) 1044 WP_338518549.1 type IV pilus twitching motility protein PilT Machinery gene
  V6N89_RS16555 - 3437637..3438335 (+) 699 WP_346992974.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6N89_RS16560 proC 3438397..3439218 (+) 822 WP_338518551.1 pyrroline-5-carboxylate reductase -
  V6N89_RS16565 - 3439240..3439779 (+) 540 WP_346992975.1 YggT family protein -
  V6N89_RS16570 - 3439836..3440276 (+) 441 WP_338518552.1 DUF4426 domain-containing protein -
  V6N89_RS16575 - 3440456..3442478 (+) 2023 Protein_3242 NADPH-dependent 2,4-dienoyl-CoA reductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38361.12 Da        Isoelectric Point: 6.5680

>NTDB_id=938800 V6N89_RS16550 WP_338518549.1 3436566..3437609(-) (pilT) [Alteromonas gracilis strain J4]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRKLNVPALDHKQVHALVYEIMNDMQRKEYEENLETDFSFEVNGLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPEIFKDIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHDNKLSLVNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVITKQDAAEKSIDKKSMSGL

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=938800 V6N89_RS16550 WP_338518549.1 3436566..3437609(-) (pilT) [Alteromonas gracilis strain J4]
GTGGATATTACCGAACTTTTGGCTTTCAGTGTTAAGAATAACGCCTCAGATTTACACTTATCAGCAGGCTTGCCCCCAAT
TATTCGTGTCGACGGAGAGATGCGTAAACTGAATGTACCAGCTCTTGATCACAAACAGGTGCACGCGCTCGTGTATGAAA
TTATGAACGACATGCAGCGTAAAGAATACGAAGAAAACTTAGAAACCGATTTCTCGTTTGAAGTGAATGGCTTGTCGCGT
TTTCGTGTAAATGCGTTTGTTCAAAATCGTGGTGCCGCTGCGGTGTTGCGTACCATCCCAAGTAAAGTGCTGACACTTGA
CGATTTAGGTGCGCCAGAAATCTTCAAAGACATCATCAACCAGCCTACGGGAATTGTGCTAGTAACCGGGGCAACGGGCT
CGGGTAAAAGTACAACATTGGCGGCAATGGTCGACCATATCAATACACACAAGCGCGAACACATTTTGACGATTGAAGAC
CCTATCGAATTTGTTCACGACAACAAACTGAGCCTAGTAAACCAGCGTGAAGTGCATCGTGACACACATTCATTTAACAA
CGCTCTTCGTTCAGCGCTGCGTGAAGACCCTGATGTTATCCTAGTAGGTGAATTACGTGACCTTGAAACCATTCGTTTGG
CGATTTCCGCAGCAGAAACGGGCCATTTAGTTTTCGGTACGCTTCATACTAACTCTGCACCCAAAACCATTGACCGTATT
ATTGACGTGTTTCCAGCGGAAGAAAAAGCCATGGTGCGTTCTATGTTGTCTGAGTCACTGCGTGCGGTTATTTCACAAAC
GCTACTTAAGAAAGTGGGCGGCGGACGAGTTGCAGCCCATGAAATCATGGTCGGTATCCCAGCTATTCGAAACCTTATTC
GTGAAGACAAAGTGCCGCAGATGTACTCTGTTATTCAAACGGGTCAGGCGCACGGTATGCAAACCATGGATCAGTGCCTG
CAGCGTTTGGTAGCAATGGGGGTCATTACTAAGCAAGATGCTGCCGAAAAATCTATTGATAAAAAATCGATGAGTGGTCT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.144

96.254

0.752
  pilT Acinetobacter baumannii strain A118

78.144

96.254

0.752
  pilT Acinetobacter nosocomialis M2

77.844

96.254

0.749
  pilT Pseudomonas stutzeri DSM 10701

76.036

97.406

0.741
  pilT Pseudomonas aeruginosa PAK

75.444

97.406

0.735
  pilT Acinetobacter baylyi ADP1

76.596

94.813

0.726
  pilT Legionella pneumophila strain Lp02

71.848

98.271

0.706
  pilT Legionella pneumophila strain ERS1305867

71.848

98.271

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.118

0.706
  pilT Vibrio cholerae strain A1552

72.7

97.118

0.706
  pilT Neisseria meningitidis 8013

65.318

99.712

0.651
  pilT Neisseria gonorrhoeae MS11

65.029

99.712

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.586

100

0.527
  pilU Pseudomonas stutzeri DSM 10701

41.379

100

0.415
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

41.124

97.406

0.401