Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V6N89_RS16545 Genome accession   NZ_CP145482
Coordinates   3435444..3436556 (-) Length   370 a.a.
NCBI ID   WP_346992973.1    Uniprot ID   -
Organism   Alteromonas gracilis strain J4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3430444..3441556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6N89_RS16515 - 3431613..3431792 (+) 180 WP_346992971.1 hypothetical protein -
  V6N89_RS16520 - 3432186..3432914 (+) 729 WP_338518546.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  V6N89_RS16525 gshB 3433071..3434025 (+) 955 Protein_3232 glutathione synthase -
  V6N89_RS16530 - 3434087..3434653 (+) 567 WP_346992972.1 YqgE/AlgH family protein -
  V6N89_RS16535 ruvX 3434646..3435077 (+) 432 WP_338518547.1 Holliday junction resolvase RuvX -
  V6N89_RS16540 - 3435084..3435413 (-) 330 WP_338518548.1 DUF2007 domain-containing protein -
  V6N89_RS16545 pilU 3435444..3436556 (-) 1113 WP_346992973.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V6N89_RS16550 pilT 3436566..3437609 (-) 1044 WP_338518549.1 type IV pilus twitching motility protein PilT Machinery gene
  V6N89_RS16555 - 3437637..3438335 (+) 699 WP_346992974.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6N89_RS16560 proC 3438397..3439218 (+) 822 WP_338518551.1 pyrroline-5-carboxylate reductase -
  V6N89_RS16565 - 3439240..3439779 (+) 540 WP_346992975.1 YggT family protein -
  V6N89_RS16570 - 3439836..3440276 (+) 441 WP_338518552.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40822.75 Da        Isoelectric Point: 6.1882

>NTDB_id=938799 V6N89_RS16545 WP_346992973.1 3435444..3436556(-) (pilU) [Alteromonas gracilis strain J4]
MLDSLLQKMVDTNASDLFVTAGFPVSAKINGKLTPITDSASSEEASLALVHEAMTEKQREEFHNSKECNFAIVREGIGRF
RCSAFWQRDQAGMVVRRIVTQIPKADDLGLPRVLKEIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKVDMDQALHHADSPNDLRLMIKLDTKDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=938799 V6N89_RS16545 WP_346992973.1 3435444..3436556(-) (pilU) [Alteromonas gracilis strain J4]
ATGTTAGATAGCTTATTACAAAAAATGGTCGACACCAACGCGTCAGATTTATTTGTGACCGCGGGCTTTCCTGTCAGTGC
CAAAATTAATGGAAAGCTAACGCCAATCACCGATTCAGCGTCTTCAGAAGAGGCCTCTCTTGCGCTAGTGCACGAGGCAA
TGACGGAAAAGCAACGCGAAGAATTTCACAACTCCAAAGAATGTAACTTCGCCATAGTCAGAGAAGGGATCGGACGTTTT
CGTTGTTCAGCATTTTGGCAACGAGACCAAGCTGGCATGGTAGTGCGTCGCATTGTGACGCAAATACCAAAAGCTGACGA
CCTAGGTCTTCCTCGGGTGCTCAAAGAAATTATCATGGCCAAACGTGGCTTGGTGCTCTTTGTGGGCGGTACAGGTACGG
GTAAATCTACGTCACTAGCGGCACTTATTGGTCATAGAAACGAAAACTCGCATGGTCATATACTAACGATTGAAGATCCT
ATCGAATTTGTTCACGAGCATAAAAACTGCGTGATTACACAACGTGAAGTAGGTATTGATACACAGTCATTTGATGACGC
GCTTAAAAGCTCACTGCGTCAAGCGCCTGATGTTATTCTCATTGGTGAGATTCGCTCCATGGAAACCATGGAATACGCCA
TGTCTTTTGCAGATACAGGGCACTTGTGCGTAGCGACGCTGCACGCCAATAATGCTAACCAAGCCATTGAACGTATTATG
CACCTTGCGCCTAAAGACCAGCACGATAAATTGCGTTTTGATTTGAGCCTAAATATTCGCGCCATTGTAGCGCAGCAACT
CGTGCCGACTCCTGATGGAAAGGGCCGTGTGGCGGCTATTGAAATTTTGCTCAACTCTCCATTGGTTCGAGACTTGATTC
AACGCAATGAGATTGGTGCATTGAAAGAGGCGATGAAAAAAGGCAAAGAGCAGGGCATGCAGAGCTTTGATATGGCGCTT
TACGACCTATATAAAGCCGGTAAAGTTGATATGGACCAAGCGCTGCATCACGCTGACTCGCCAAATGATTTGCGTCTAAT
GATTAAACTAGACACTAAAGATGGCTCTAGCTTGGGCACATTGTCGAATGTTTCCATTGATATGGATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.108

100

0.603
  pilU Acinetobacter baylyi ADP1

56.438

98.649

0.557
  pilU Vibrio cholerae strain A1552

55.163

99.459

0.549
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.059

91.892

0.386
  pilT Acinetobacter nosocomialis M2

39.706

91.892

0.365
  pilT Acinetobacter baumannii D1279779

39.706

91.892

0.365
  pilT Acinetobacter baumannii strain A118

39.706

91.892

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.729

88.919

0.362
  pilT Vibrio cholerae strain A1552

40.729

88.919

0.362