Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SNR28_RS11545 Genome accession   NZ_CP145311
Coordinates   426035..427081 (+) Length   348 a.a.
NCBI ID   WP_375752545.1    Uniprot ID   -
Organism   Vibrio sp. HN007     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421035..432081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SNR28_RS11515 (SNR28_11500) hemW 421379..422530 (-) 1152 WP_375752536.1 radical SAM family heme chaperone HemW -
  SNR28_RS11520 (SNR28_11505) - 422731..423318 (-) 588 WP_375752538.1 XTP/dITP diphosphatase -
  SNR28_RS11525 (SNR28_11510) yggU 423578..423865 (-) 288 WP_375752539.1 DUF167 family protein YggU -
  SNR28_RS11530 (SNR28_11515) - 423865..424422 (-) 558 WP_375752541.1 YggT family protein -
  SNR28_RS11535 (SNR28_11520) proC 424456..425274 (-) 819 WP_375752543.1 pyrroline-5-carboxylate reductase -
  SNR28_RS11540 (SNR28_11525) - 425301..426011 (-) 711 WP_375752544.1 YggS family pyridoxal phosphate-dependent enzyme -
  SNR28_RS11545 (SNR28_11530) pilT 426035..427081 (+) 1047 WP_375752545.1 type IV pilus twitching motility protein PilT Machinery gene
  SNR28_RS11550 (SNR28_11535) pilU 427093..428199 (+) 1107 WP_375752546.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SNR28_RS11555 (SNR28_11540) ruvX 428220..428642 (-) 423 WP_375752547.1 Holliday junction resolvase RuvX -
  SNR28_RS11560 (SNR28_11545) - 428642..429205 (-) 564 WP_375752548.1 YqgE/AlgH family protein -
  SNR28_RS11565 (SNR28_11550) gshB 429231..430178 (-) 948 WP_375752550.1 glutathione synthase -
  SNR28_RS11570 (SNR28_11555) rsmE 430192..430923 (-) 732 WP_375752551.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SNR28_RS11575 (SNR28_11560) - 431011..431517 (-) 507 WP_375752553.1 SprT family zinc-dependent metalloprotease -
  SNR28_RS11580 (SNR28_11565) mog 431525..432058 (-) 534 WP_375752555.1 molybdopterin adenylyltransferase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38486.96 Da        Isoelectric Point: 6.3173

>NTDB_id=938400 SNR28_RS11545 WP_375752545.1 426035..427081(+) (pilT) [Vibrio sp. HN007]
MDIAELLQFSVKHNASDLHLSAGVPPMIRIDGDVRKLGVPAFSHSDVHKLVFDIMNDAQRAEYEEKLEVDFSFELPQIGR
FRVNAFNQSRGCAAVFRTIPTRIPSLDELEAPDIFNEIANYEKGLVLVTGPTGSGKSTTLAAMVDYINQHHNKHILTIED
PIEFVHENSKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEDKNMVRSMLSESLRAVIAQKLLKRIGGGRVACHEVMIANSAIRNLIREDKVAQMQSIIQTGAATGMKTMEQGA
KQLLAQGQVEQQEVNKKIETTTLSVTGF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=938400 SNR28_RS11545 WP_375752545.1 426035..427081(+) (pilT) [Vibrio sp. HN007]
ATGGATATCGCCGAATTACTGCAATTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCAGGTGTACCTCCTAT
GATAAGGATAGATGGTGATGTAAGAAAGCTCGGAGTTCCAGCATTTAGTCATTCAGACGTCCATAAGTTAGTGTTTGATA
TTATGAATGATGCACAAAGAGCAGAATATGAGGAAAAATTGGAAGTCGATTTCTCGTTCGAGTTGCCTCAGATTGGGCGC
TTTCGTGTCAATGCGTTTAACCAGAGCAGAGGCTGCGCAGCGGTATTCAGAACGATTCCTACCCGTATTCCATCTCTTGA
TGAACTGGAAGCACCAGATATTTTTAATGAAATTGCTAATTATGAAAAAGGGTTGGTTTTAGTTACAGGTCCCACAGGCT
CAGGCAAATCAACTACATTAGCTGCGATGGTGGACTACATCAATCAGCATCACAACAAGCATATTCTGACTATTGAAGAC
CCTATTGAGTTTGTGCATGAAAACAGCAAATGTCTGATAAACCAAAGAGAAGTTCATAGAGACACTCATAGCTTCAAAGC
GGCCCTTCGTTCAGCCCTTCGAGAAGATCCGGATGTTATTTTGGTGGGAGAGCTTCGTGATCAGGAAACGATCAGTCTGG
CACTGACAGCCGCTGAAACAGGGCACCTGGTTTTCGGTACTTTGCATACCAGCTCTGCAGCAAAAACCATTGATCGAATT
ATTGATGTATTTCCTGGTGAAGATAAAAATATGGTTCGCTCTATGCTTTCTGAATCCTTGCGGGCCGTTATCGCTCAGAA
ACTGTTAAAGCGTATTGGGGGAGGCCGTGTCGCGTGTCATGAAGTCATGATTGCTAATAGTGCTATTCGAAACCTTATTC
GGGAAGATAAAGTTGCTCAAATGCAGTCAATCATTCAAACCGGAGCAGCAACTGGTATGAAGACTATGGAGCAGGGAGCA
AAACAGCTTCTGGCTCAGGGTCAGGTAGAGCAACAGGAAGTGAACAAGAAAATAGAAACAACCACCTTATCTGTTACCGG
CTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.726

97.414

0.845
  pilT Vibrio cholerae strain A1552

86.726

97.414

0.845
  pilT Acinetobacter baylyi ADP1

73.089

93.966

0.687
  pilT Acinetobacter baumannii strain A118

71.084

95.402

0.678
  pilT Acinetobacter baumannii D1279779

71.084

95.402

0.678
  pilT Acinetobacter nosocomialis M2

70.783

95.402

0.675
  pilT Pseudomonas stutzeri DSM 10701

67.953

96.839

0.658
  pilT Legionella pneumophila strain Lp02

68.675

95.402

0.655
  pilT Legionella pneumophila strain ERS1305867

68.675

95.402

0.655
  pilT Pseudomonas aeruginosa PAK

67.359

96.839

0.652
  pilT Neisseria meningitidis 8013

63.158

98.276

0.621
  pilT Neisseria gonorrhoeae MS11

62.865

98.276

0.618
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.088

91.379

0.494
  pilU Vibrio cholerae strain A1552

42.515

95.977

0.408
  pilU Pseudomonas stutzeri DSM 10701

40.719

95.977

0.391
  pilU Acinetobacter baylyi ADP1

41.121

92.241

0.379