Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SNR28_RS11550 Genome accession   NZ_CP145311
Coordinates   427093..428199 (+) Length   368 a.a.
NCBI ID   WP_375752546.1    Uniprot ID   -
Organism   Vibrio sp. HN007     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422093..433199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SNR28_RS11520 (SNR28_11505) - 422731..423318 (-) 588 WP_375752538.1 XTP/dITP diphosphatase -
  SNR28_RS11525 (SNR28_11510) yggU 423578..423865 (-) 288 WP_375752539.1 DUF167 family protein YggU -
  SNR28_RS11530 (SNR28_11515) - 423865..424422 (-) 558 WP_375752541.1 YggT family protein -
  SNR28_RS11535 (SNR28_11520) proC 424456..425274 (-) 819 WP_375752543.1 pyrroline-5-carboxylate reductase -
  SNR28_RS11540 (SNR28_11525) - 425301..426011 (-) 711 WP_375752544.1 YggS family pyridoxal phosphate-dependent enzyme -
  SNR28_RS11545 (SNR28_11530) pilT 426035..427081 (+) 1047 WP_375752545.1 type IV pilus twitching motility protein PilT Machinery gene
  SNR28_RS11550 (SNR28_11535) pilU 427093..428199 (+) 1107 WP_375752546.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SNR28_RS11555 (SNR28_11540) ruvX 428220..428642 (-) 423 WP_375752547.1 Holliday junction resolvase RuvX -
  SNR28_RS11560 (SNR28_11545) - 428642..429205 (-) 564 WP_375752548.1 YqgE/AlgH family protein -
  SNR28_RS11565 (SNR28_11550) gshB 429231..430178 (-) 948 WP_375752550.1 glutathione synthase -
  SNR28_RS11570 (SNR28_11555) rsmE 430192..430923 (-) 732 WP_375752551.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SNR28_RS11575 (SNR28_11560) - 431011..431517 (-) 507 WP_375752553.1 SprT family zinc-dependent metalloprotease -
  SNR28_RS11580 (SNR28_11565) mog 431525..432058 (-) 534 WP_375752555.1 molybdopterin adenylyltransferase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41163.05 Da        Isoelectric Point: 5.6213

>NTDB_id=938401 SNR28_RS11550 WP_375752546.1 427093..428199(+) (pilU) [Vibrio sp. HN007]
MLLDSALNQMIENKASDLYITVGAPCFLRIDGELKEISASMDEEHVFQVLDSCMDTERKQEFLGTKEANFAIVRDNGRFR
VSAFFQRELPGAVIRRIETEIPTIEQLHLPATMKDLAVAKRGLVLVVGATGSGKSTTMAAMTGYRNEQRSGHILTVEDPI
EFVHQHNKCIVTQREVGLDTESFEVALKNSLRQAPDMILIGEIRTRETMEYAMNFAETGHLCMATLHANNANQALERILN
LVPKDAREQFLFDLSVNLKGIIGQQLIRDKNGKGRHGCFELLLNTPRVSDLIRQGNLHEIKAAMSRSNESGMMTFDQSLY
QLVSKDKISEEDALHNADSANDLRMMLKIKRGDIAGGNSLDHVTIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=938401 SNR28_RS11550 WP_375752546.1 427093..428199(+) (pilU) [Vibrio sp. HN007]
ATGCTACTGGATTCTGCGCTCAATCAAATGATCGAAAACAAGGCGTCTGATCTTTATATCACTGTTGGTGCACCATGCTT
TTTACGTATCGATGGTGAGTTAAAAGAGATTAGTGCATCCATGGACGAAGAGCATGTGTTTCAGGTTCTTGACTCTTGTA
TGGATACCGAGCGTAAACAAGAGTTTTTGGGCACGAAAGAGGCGAACTTCGCCATTGTTCGTGATAATGGCCGATTTCGG
GTCAGCGCTTTTTTCCAGCGAGAACTTCCCGGTGCAGTCATTCGTCGAATTGAAACAGAAATTCCGACGATTGAACAACT
TCACCTACCTGCAACAATGAAAGATTTGGCAGTAGCTAAACGAGGACTGGTGTTGGTAGTCGGGGCGACAGGCTCGGGTA
AGTCGACAACCATGGCTGCTATGACTGGGTACAGGAATGAGCAGAGAAGCGGTCATATTCTGACGGTAGAAGACCCTATT
GAGTTTGTGCATCAACATAATAAGTGCATTGTGACTCAGCGTGAGGTTGGGCTTGATACGGAGAGCTTTGAAGTTGCATT
AAAGAATTCGCTAAGGCAGGCTCCGGATATGATTCTGATTGGTGAAATTCGTACTCGTGAAACCATGGAGTACGCAATGA
ACTTTGCTGAAACAGGTCACCTCTGTATGGCTACTCTGCATGCAAACAACGCTAATCAGGCGTTAGAGCGAATTTTGAAT
CTGGTTCCTAAGGATGCCAGAGAGCAGTTTCTGTTTGATCTTTCGGTAAATTTAAAGGGCATTATCGGCCAACAGCTTAT
TCGTGACAAAAATGGTAAAGGGCGTCACGGTTGCTTCGAGTTGCTGCTGAACACACCTCGTGTATCTGATTTAATTCGAC
AAGGTAATTTACATGAGATTAAAGCGGCAATGAGTCGTTCAAATGAGAGTGGAATGATGACGTTTGATCAGTCACTTTAT
CAGCTTGTGTCGAAAGATAAGATCTCTGAAGAAGATGCTCTGCATAATGCAGATTCTGCAAATGACTTACGTATGATGTT
GAAAATTAAGCGTGGGGATATTGCTGGTGGAAATTCGCTAGATCATGTAACGATAGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

74.457

100

0.745
  pilU Pseudomonas stutzeri DSM 10701

57.835

95.38

0.552
  pilU Acinetobacter baylyi ADP1

54.83

95.652

0.524
  pilT Legionella pneumophila strain ERS1305867

42.154

88.315

0.372
  pilT Legionella pneumophila strain Lp02

42.154

88.315

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.265

90.217

0.372
  pilT Acinetobacter nosocomialis M2

41.538

88.315

0.367
  pilT Acinetobacter baumannii D1279779

41.231

88.315

0.364
  pilT Acinetobacter baumannii strain A118

41.231

88.315

0.364