Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NGK70_RS19685 Genome accession   NZ_AP025730
Coordinates   4614239..4615282 (-) Length   347 a.a.
NCBI ID   WP_251970175.1    Uniprot ID   -
Organism   Sphaerotilus microaerophilus strain FB-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4609239..4620282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGK70_RS19660 (CATMQ487_39050) - 4610324..4610683 (+) 360 WP_251970170.1 YraN family protein -
  NGK70_RS19665 (CATMQ487_39060) - 4610783..4611370 (+) 588 WP_251970171.1 SIS domain-containing protein -
  NGK70_RS19670 (CATMQ487_39070) - 4611373..4612032 (+) 660 WP_251970172.1 BON domain-containing protein -
  NGK70_RS19675 (CATMQ487_39080) - 4612033..4612953 (-) 921 WP_251970173.1 NAD(P)-dependent oxidoreductase -
  NGK70_RS19680 (CATMQ487_39090) pilU 4613027..4614163 (-) 1137 WP_251970174.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NGK70_RS19685 (CATMQ487_39100) pilT 4614239..4615282 (-) 1044 WP_251970175.1 type IV pilus twitching motility protein PilT Machinery gene
  NGK70_RS19690 (CATMQ487_39110) - 4615446..4616135 (+) 690 WP_251970176.1 YggS family pyridoxal phosphate-dependent enzyme -
  NGK70_RS19695 (CATMQ487_39120) proC 4616167..4616988 (+) 822 WP_251970177.1 pyrroline-5-carboxylate reductase -
  NGK70_RS19700 (CATMQ487_39130) - 4617098..4617316 (+) 219 WP_251970178.1 hypothetical protein -
  NGK70_RS19705 (CATMQ487_39140) - 4617482..4618795 (-) 1314 WP_251973838.1 IS1182 family transposase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38356.98 Da        Isoelectric Point: 7.5440

>NTDB_id=93806 NGK70_RS19685 WP_251970175.1 4614239..4615282(-) (pilT) [Sphaerotilus microaerophilus strain FB-5]
MDITQLLAFSVRNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKKVREMIYDVMNDAQRKVYEETLECDFSFEIQGLSR
FRVNAFNQQRGAGAVFRTIPSKIQSLEELRTPKVFADLSLKPRGLLLVTGPTGSGKSTTLAAMVDHRNDNEHGHILTVED
PIEFVHESKKCLINQREVGPQTLSFSNALRAALREDPDAILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTVDRM
VDVFPAAEKEMVRAMLSESLVAVISQTLCKNRDGTGRVAAHEIMLGSAAIRNLIRENKVAQMYSIIQTGQAQGMQTLDQN
LTELVRKGVITPAEARSKARSPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=93806 NGK70_RS19685 WP_251970175.1 4614239..4615282(-) (pilT) [Sphaerotilus microaerophilus strain FB-5]
ATGGACATCACTCAGTTGCTCGCGTTCTCGGTGCGCAACAAGGCCTCTGACCTCCACCTCTCGGCCGGCCTGCCGCCCAT
GATCCGCGTGCACGGCGACGTGCGGCGCATCAACGTCGACCCGCTGGACCACAAGAAGGTCCGGGAGATGATCTACGACG
TGATGAACGACGCCCAGCGCAAGGTCTACGAGGAGACGCTGGAATGCGATTTCTCCTTCGAGATCCAGGGCCTGTCGCGC
TTTCGCGTCAACGCCTTCAATCAGCAGCGGGGCGCCGGCGCGGTGTTCCGCACCATCCCGAGCAAGATCCAGAGCCTGGA
GGAGCTGCGCACGCCCAAGGTCTTCGCCGACCTGTCGCTCAAGCCGCGCGGGCTGCTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAATCCACCACGCTGGCGGCCATGGTGGATCACCGCAACGACAACGAGCATGGGCACATCCTGACGGTCGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGGGGCCGCAGACGCTGTCGTTCTCCAA
TGCGCTGCGTGCCGCGCTGCGCGAGGATCCGGACGCCATCCTGGTGGGCGAGTTGCGCGACCTGGAGACCATCCGCCTCG
CACTCACCGCGGCGGAGACCGGCCACCTCGTCTTTGCCACGCTGCACACCTCGAGCGCGGCCAAGACCGTCGACCGGATG
GTGGACGTCTTCCCCGCGGCCGAAAAGGAGATGGTGCGCGCGATGCTCTCGGAGTCGCTGGTGGCGGTGATCTCGCAGAC
GCTGTGCAAGAACCGCGACGGCACCGGACGGGTGGCGGCGCACGAGATCATGCTGGGCTCGGCGGCCATCCGCAACCTGA
TCCGCGAGAACAAGGTGGCCCAGATGTACTCGATCATCCAGACCGGGCAGGCCCAGGGCATGCAGACGCTGGACCAGAAC
CTGACCGAGCTGGTGCGCAAAGGGGTGATCACGCCCGCCGAGGCCCGCAGCAAGGCCCGCAGCCCCGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.623

99.424

0.732
  pilT Acinetobacter nosocomialis M2

72.174

99.424

0.718
  pilT Acinetobacter baumannii D1279779

72.174

99.424

0.718
  pilT Acinetobacter baumannii strain A118

72.174

99.424

0.718
  pilT Acinetobacter baylyi ADP1

72.174

99.424

0.718
  pilT Pseudomonas stutzeri DSM 10701

72.174

99.424

0.718
  pilT Legionella pneumophila strain Lp02

69.275

99.424

0.689
  pilT Legionella pneumophila strain ERS1305867

69.275

99.424

0.689
  pilT Neisseria meningitidis 8013

66.377

99.424

0.66
  pilT Neisseria gonorrhoeae MS11

66.087

99.424

0.657
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.976

97.406

0.643
  pilT Vibrio cholerae strain A1552

65.976

97.406

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Pseudomonas stutzeri DSM 10701

41.739

99.424

0.415
  pilU Vibrio cholerae strain A1552

41.739

99.424

0.415
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403

Multiple sequence alignment