Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NGK70_RS19680 Genome accession   NZ_AP025730
Coordinates   4613027..4614163 (-) Length   378 a.a.
NCBI ID   WP_251970174.1    Uniprot ID   -
Organism   Sphaerotilus microaerophilus strain FB-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4608027..4619163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGK70_RS19650 (CATMQ487_39030) - 4608042..4608950 (-) 909 WP_251970169.1 transporter substrate-binding domain-containing protein -
  NGK70_RS19655 (CATMQ487_39040) rsmI 4609189..4610244 (-) 1056 WP_251973837.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  NGK70_RS19660 (CATMQ487_39050) - 4610324..4610683 (+) 360 WP_251970170.1 YraN family protein -
  NGK70_RS19665 (CATMQ487_39060) - 4610783..4611370 (+) 588 WP_251970171.1 SIS domain-containing protein -
  NGK70_RS19670 (CATMQ487_39070) - 4611373..4612032 (+) 660 WP_251970172.1 BON domain-containing protein -
  NGK70_RS19675 (CATMQ487_39080) - 4612033..4612953 (-) 921 WP_251970173.1 NAD(P)-dependent oxidoreductase -
  NGK70_RS19680 (CATMQ487_39090) pilU 4613027..4614163 (-) 1137 WP_251970174.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NGK70_RS19685 (CATMQ487_39100) pilT 4614239..4615282 (-) 1044 WP_251970175.1 type IV pilus twitching motility protein PilT Machinery gene
  NGK70_RS19690 (CATMQ487_39110) - 4615446..4616135 (+) 690 WP_251970176.1 YggS family pyridoxal phosphate-dependent enzyme -
  NGK70_RS19695 (CATMQ487_39120) proC 4616167..4616988 (+) 822 WP_251970177.1 pyrroline-5-carboxylate reductase -
  NGK70_RS19700 (CATMQ487_39130) - 4617098..4617316 (+) 219 WP_251970178.1 hypothetical protein -
  NGK70_RS19705 (CATMQ487_39140) - 4617482..4618795 (-) 1314 WP_251973838.1 IS1182 family transposase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42199.50 Da        Isoelectric Point: 6.8816

>NTDB_id=93805 NGK70_RS19680 WP_251970174.1 4613027..4614163(-) (pilU) [Sphaerotilus microaerophilus strain FB-5]
MERDQASKFINDLLRLLVSRAGSDLFLTCDFPPAIKVDGKVTKVSPQPLTGQHTLALARSVMNDKQAAEFERTKECNFAI
SPSAVGRFRVNAFMQQGQVGMVFRTIPRELPTIDKLGLPTVLKDVTMSKRGLVIMVGATGSGKSTSLAAMVDYRNETAYD
HIITIEDPVEFVHPHKNCIITQREVGIDTLNWESALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIVNFFPEERRDQLLMDLSLNLRAMISQRLLPREEGAGRIAAVEVLLNTPLVSELIFKGEVSAIKEIMKKSREHG
MQTFDQSLFDLYEGHYIGYADALRNADSVNDLRLQIKLHSRRARNTDFGAGTEHLAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=93805 NGK70_RS19680 WP_251970174.1 4613027..4614163(-) (pilU) [Sphaerotilus microaerophilus strain FB-5]
ATGGAACGCGATCAGGCTTCGAAATTCATCAATGACTTGCTGCGGCTGCTGGTGTCGCGTGCCGGGTCGGACCTGTTCCT
GACCTGTGACTTCCCGCCGGCGATCAAGGTGGACGGCAAGGTGACCAAGGTGTCGCCGCAGCCGCTGACCGGCCAGCACA
CGCTGGCGTTGGCCCGCTCGGTGATGAACGACAAGCAGGCGGCCGAGTTCGAGCGCACCAAGGAGTGCAATTTCGCCATC
TCGCCGTCCGCGGTGGGGCGCTTCCGGGTCAATGCCTTCATGCAGCAGGGGCAGGTGGGCATGGTGTTCCGCACCATCCC
GCGCGAGCTGCCGACGATCGACAAGCTCGGCCTGCCGACGGTGCTCAAGGACGTGACCATGTCCAAGCGCGGGCTGGTCA
TCATGGTGGGCGCCACCGGCTCGGGCAAGAGCACCTCGCTGGCGGCGATGGTGGACTACCGCAACGAAACCGCCTACGAC
CACATCATCACCATCGAGGATCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCATCATCACTCAGCGCGAGGTCGGCAT
CGACACCCTGAACTGGGAGTCCGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGAGCACGCGGTGGCCTTCGCCGAGACCGGTCACCTGTGCATGGCCACCCTGCATGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTTCCCGAAGAACGGCGCGACCAGCTGCTGATGGACCTGTCGTTGAACCT
GCGCGCCATGATCTCGCAGCGCCTGCTGCCGCGCGAGGAAGGCGCCGGGCGCATCGCCGCGGTGGAGGTGCTGCTGAACA
CGCCGCTGGTGTCCGAGCTGATCTTCAAGGGTGAGGTCTCTGCCATCAAGGAGATCATGAAGAAGAGCCGCGAGCACGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTACGAAGGCCACTACATCGGCTACGCCGATGCCCTGCGCAACGCCGA
TTCGGTCAACGACCTGCGCCTGCAGATCAAGCTGCACAGCCGGCGCGCCCGCAACACCGACTTCGGCGCCGGTACCGAGC
ACCTGGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.207

92.063

0.563
  pilU Acinetobacter baylyi ADP1

58.739

92.328

0.542
  pilU Vibrio cholerae strain A1552

51.791

96.032

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.774

89.153

0.426
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Acinetobacter baylyi ADP1

45.652

85.185

0.389
  pilT Acinetobacter baumannii D1279779

45.768

84.392

0.386
  pilT Acinetobacter baumannii strain A118

45.768

84.392

0.386
  pilT Acinetobacter nosocomialis M2

45.768

84.392

0.386
  pilT Pseudomonas stutzeri DSM 10701

45.455

84.392

0.384
  pilT Vibrio cholerae strain A1552

44.654

84.127

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.654

84.127

0.376
  pilT Neisseria meningitidis 8013

41.003

89.683

0.368
  pilT Neisseria gonorrhoeae MS11

41.003

89.683

0.368
  pilT Legionella pneumophila strain Lp02

42.249

87.037

0.368
  pilT Legionella pneumophila strain ERS1305867

42.249

87.037

0.368

Multiple sequence alignment