Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NUH87_RS19095 Genome accession   NZ_CP144470
Coordinates   4220073..4221107 (+) Length   344 a.a.
NCBI ID   WP_040071484.1    Uniprot ID   A0A0C2HUK8
Organism   Pseudomonas batumici strain Koz11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4215073..4226107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUH87_RS19065 (NUH87_19065) - 4215452..4215886 (-) 435 WP_338522273.1 DUF4426 domain-containing protein -
  NUH87_RS19070 (NUH87_19070) metW 4215914..4216534 (-) 621 WP_040071489.1 methionine biosynthesis protein MetW -
  NUH87_RS19075 (NUH87_19075) - 4216542..4217681 (-) 1140 WP_040071488.1 homoserine O-acetyltransferase -
  NUH87_RS19080 (NUH87_19080) - 4217863..4218453 (-) 591 WP_338522274.1 YggT family protein -
  NUH87_RS19085 (NUH87_19085) proC 4218472..4219296 (-) 825 WP_040071486.1 pyrroline-5-carboxylate reductase -
  NUH87_RS19090 (NUH87_19090) - 4219330..4220016 (-) 687 WP_338522275.1 YggS family pyridoxal phosphate-dependent enzyme -
  NUH87_RS19095 (NUH87_19095) pilT 4220073..4221107 (+) 1035 WP_040071484.1 type IV pilus twitching motility protein PilT Machinery gene
  NUH87_RS19100 (NUH87_19100) pilU 4221172..4222278 (+) 1107 WP_338522276.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NUH87_RS19105 (NUH87_19105) - 4222330..4222974 (-) 645 WP_040071482.1 C40 family peptidase -
  NUH87_RS19110 (NUH87_19110) - 4223225..4223644 (+) 420 WP_338522277.1 TM2 domain-containing protein -
  NUH87_RS19115 (NUH87_19115) - 4223691..4224962 (-) 1272 WP_338522278.1 dihydroorotase -
  NUH87_RS19120 (NUH87_19120) - 4224959..4225963 (-) 1005 WP_040071479.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38086.79 Da        Isoelectric Point: 7.1515

>NTDB_id=933991 NUH87_RS19095 WP_040071484.1 4220073..4221107(+) (pilT) [Pseudomonas batumici strain Koz11]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHDLIYDIMNDRQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGSGAVFRTIPSKVLTMSDLGMGEVFRKITEVPRGLVLVTGPTGSGKSTTLAAMIDHLNNNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTQSFAAALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKSMVRSMLSESLQAVISQSLLKKVGGGRIAAHEIMLGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KDLLTKGLITRDNAREKAKIPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=933991 NUH87_RS19095 WP_040071484.1 4220073..4221107(+) (pilT) [Pseudomonas batumici strain Koz11]
ATGGATATCACCGAGCTGCTGGCCTTTAGCGCCAAACAGGGCGCGTCGGACTTGCACCTCTCGGCGGGCCTGCCGCCGAT
GATCCGGGTTGACGGCGATGTGCGGCGGATCAACCTGCCGGCGCTGGACCACAAGCAAGTCCACGACCTGATCTACGACA
TCATGAACGACCGCCAGCGCAAGGACTACGAGGAGTTCCTGGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCACAACCGCGGTTCGGGCGCGGTGTTCCGCACCATTCCCTCAAAAGTCCTGACCATGAG
CGACCTGGGCATGGGTGAGGTGTTCCGCAAGATCACCGAGGTGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCAGCGATGATCGACCACCTGAACAACAACAAGCATCACCATATCCTGACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGTGAAGTGCACCGCGACACCCAGAGTTTCGCCGC
CGCCCTGCGTTCGGCCCTGCGTGAGGACCCGGACGTGATCCTGGTGGGTGAGATGCGCGACCTGGAAACCATCCGCCTGG
CCCTCACCGCCGCCGAAACCGGTCACCTGGTGTTCGGCACGCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTTCCGGCGCAAGAGAAGTCCATGGTCCGCTCGATGCTTTCCGAATCCCTGCAGGCGGTGATTTCCCAGAG
CCTGCTGAAGAAGGTCGGCGGCGGGCGCATCGCGGCCCATGAGATCATGCTCGGCACGCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGTGGCTCGCTGGGCATGCAGACCCTGGACATGTGCCTC
AAGGACCTGCTGACCAAGGGCCTGATTACCCGTGACAACGCCCGGGAGAAGGCGAAGATCCCGGACAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C2HUK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

91.279

100

0.913
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

73.669

98.256

0.724
  pilT Legionella pneumophila strain ERS1305867

73.669

98.256

0.724
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Neisseria meningitidis 8013

67.647

98.837

0.669
  pilT Neisseria gonorrhoeae MS11

67.353

98.837

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Vibrio cholerae strain A1552

39.823

98.547

0.392