Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NUH87_RS19100 Genome accession   NZ_CP144470
Coordinates   4221172..4222278 (+) Length   368 a.a.
NCBI ID   WP_338522276.1    Uniprot ID   -
Organism   Pseudomonas batumici strain Koz11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4216172..4227278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUH87_RS19075 (NUH87_19075) - 4216542..4217681 (-) 1140 WP_040071488.1 homoserine O-acetyltransferase -
  NUH87_RS19080 (NUH87_19080) - 4217863..4218453 (-) 591 WP_338522274.1 YggT family protein -
  NUH87_RS19085 (NUH87_19085) proC 4218472..4219296 (-) 825 WP_040071486.1 pyrroline-5-carboxylate reductase -
  NUH87_RS19090 (NUH87_19090) - 4219330..4220016 (-) 687 WP_338522275.1 YggS family pyridoxal phosphate-dependent enzyme -
  NUH87_RS19095 (NUH87_19095) pilT 4220073..4221107 (+) 1035 WP_040071484.1 type IV pilus twitching motility protein PilT Machinery gene
  NUH87_RS19100 (NUH87_19100) pilU 4221172..4222278 (+) 1107 WP_338522276.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NUH87_RS19105 (NUH87_19105) - 4222330..4222974 (-) 645 WP_040071482.1 C40 family peptidase -
  NUH87_RS19110 (NUH87_19110) - 4223225..4223644 (+) 420 WP_338522277.1 TM2 domain-containing protein -
  NUH87_RS19115 (NUH87_19115) - 4223691..4224962 (-) 1272 WP_338522278.1 dihydroorotase -
  NUH87_RS19120 (NUH87_19120) - 4224959..4225963 (-) 1005 WP_040071479.1 aspartate carbamoyltransferase catalytic subunit -
  NUH87_RS19125 (NUH87_19125) pyrR 4225976..4226482 (-) 507 WP_040071478.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NUH87_RS19130 (NUH87_19130) ruvX 4226550..4226990 (-) 441 WP_040071477.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40789.24 Da        Isoelectric Point: 6.9730

>NTDB_id=933992 NUH87_RS19100 WP_338522276.1 4221172..4222278(+) (pilU) [Pseudomonas batumici strain Koz11]
MEFEKLLRLMVEKGGSDLFITPGLPPSLKVNGQILPVTRTPLTHEQTQDAVFGLMNERQKLDFAHLHECNFAIHPKGIGR
FRVSAFYQRSQPGMVLRRIETRIPGLAELKLPPILEQLALLKRGLVMLVGATGAGKSTSMAAMIGHRNQQGSGHIVTVED
PIEFVHEHQNCIVTQREVGIDTDSYEIALKNTLRQAPDLIMIGEVRTRETMEHALAFAETGHLCLATLHAPNADQALERI
ISFFPVDRHPQIWMELSLNLRAIVAQQLIPTQDGQGRRAVVEVLLNTPLMADLIRKGEIKELKALMKRSTEQGMQTLDQA
LYALHQEGAISYDDALAYADSANDLRLMIKLEKGARGKGADNLSLGPA

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=933992 NUH87_RS19100 WP_338522276.1 4221172..4222278(+) (pilU) [Pseudomonas batumici strain Koz11]
ATGGAATTCGAGAAACTGCTGCGCCTGATGGTGGAGAAGGGCGGTTCCGACCTGTTCATCACCCCGGGCCTGCCGCCGTC
GCTGAAGGTCAACGGCCAGATCCTGCCGGTGACCCGTACGCCGCTGACCCACGAGCAGACCCAGGACGCGGTGTTCGGGC
TGATGAACGAGCGTCAGAAGCTGGACTTCGCCCATCTGCACGAATGCAATTTCGCCATCCATCCCAAGGGCATTGGCCGC
TTCCGGGTCAGCGCCTTCTACCAGCGCAGCCAACCGGGCATGGTGTTGCGGCGCATCGAGACACGGATTCCCGGGCTGGC
CGAACTGAAACTGCCGCCGATCCTCGAGCAACTGGCGCTGCTCAAGCGCGGGCTGGTGATGCTCGTCGGGGCGACCGGAG
CCGGCAAGTCGACCTCCATGGCGGCGATGATCGGTCACCGCAACCAGCAGGGCAGCGGCCATATCGTCACGGTCGAGGAC
CCCATCGAGTTCGTCCACGAACACCAGAACTGCATCGTGACCCAGCGGGAAGTGGGGATCGACACCGACTCCTACGAGAT
TGCGCTGAAAAACACCCTGCGCCAGGCCCCGGACCTGATCATGATCGGTGAAGTGCGTACCCGCGAAACCATGGAGCACG
CCCTGGCCTTCGCGGAAACCGGGCACCTGTGCCTGGCGACCCTGCATGCGCCGAATGCCGACCAGGCGCTGGAACGGATC
ATCAGCTTCTTTCCCGTGGACCGGCATCCGCAGATCTGGATGGAGCTGTCACTCAACCTGCGGGCGATTGTCGCCCAGCA
ACTGATTCCGACCCAGGATGGCCAGGGGCGGCGAGCGGTGGTGGAAGTGTTGCTGAATACGCCACTGATGGCCGACCTGA
TTCGCAAGGGCGAGATCAAGGAGCTCAAGGCGCTGATGAAGCGCTCGACCGAACAGGGGATGCAGACGTTGGATCAGGCG
CTGTATGCGTTGCATCAGGAAGGGGCGATCAGCTACGACGACGCCCTGGCGTACGCAGACTCGGCCAATGACTTGCGGTT
GATGATCAAGCTGGAAAAGGGCGCGAGAGGCAAGGGGGCGGATAATTTGAGCCTGGGGCCGGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

72.404

99.457

0.72
  pilU Acinetobacter baylyi ADP1

61.983

98.641

0.611
  pilU Vibrio cholerae strain A1552

58.192

96.196

0.56
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

92.12

0.383