Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VZ068_RS14575 Genome accession   NZ_CP144460
Coordinates   3464391..3465428 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas sp. 10-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3459391..3470428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZ068_RS14550 (VZ068_14555) - 3460508..3461047 (+) 540 WP_259151738.1 DNA-3-methyladenine glycosylase I -
  VZ068_RS14560 (VZ068_14565) - 3461599..3462273 (-) 675 WP_349657717.1 YitT family protein -
  VZ068_RS14565 (VZ068_14570) - 3462546..3463127 (-) 582 WP_349655698.1 MFS transporter permease -
  VZ068_RS14570 (VZ068_14575) pilU 3463139..3464269 (-) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VZ068_RS14575 (VZ068_14580) pilT 3464391..3465428 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  VZ068_RS14580 (VZ068_14585) - 3465757..3466449 (+) 693 WP_349655699.1 YggS family pyridoxal phosphate-dependent enzyme -
  VZ068_RS14585 (VZ068_14590) proC 3466499..3467353 (+) 855 WP_349655700.1 pyrroline-5-carboxylate reductase -
  VZ068_RS14590 (VZ068_14595) - 3467875..3468294 (+) 420 WP_039404742.1 HU family DNA-binding protein -
  VZ068_RS14595 (VZ068_14600) - 3468426..3469043 (+) 618 WP_349655701.1 M23 family metallopeptidase -
  VZ068_RS14600 (VZ068_14605) - 3469135..3469437 (+) 303 WP_259151823.1 DUF3861 domain-containing protein -
  VZ068_RS14605 (VZ068_14610) - 3469714..3470034 (+) 321 WP_259151824.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=933958 VZ068_RS14575 WP_003489919.1 3464391..3465428(-) (pilT) [Xanthomonas sp. 10-10]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=933958 VZ068_RS14575 WP_003489919.1 3464391..3465428(-) (pilT) [Xanthomonas sp. 10-10]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCGTTGGACCATAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAAGAGTTCCTCGAGGTCGACTTCTCGTTCGAGATTCCCTCGCTGGCGCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGTGGCGCTGGTGCGGTGTTCCGCACCATTCCGTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGTCTGATCCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGTCTGATCAACCAGCGCGAAGTGCACCGCGATACGCACGGCTTCAACGA
AGCGCTGCGCTCGGCCTTGCGCGAAGACCCGGACATCATCCTGGTTGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTCACCGCTGCGGAAACCGGTCACCTGGTGTTCGGTACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCCCTGCGCGCGGTGATTTCGCAGGC
ATTGCTCAAGAAAGTGGGCGGCGGGCGTACCGCGGCCTGGGAAATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGTCAGCAGTACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGTAGCCTGATCACGCGCAACCAGGCCCGTGAATACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394