Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VZ068_RS14570 Genome accession   NZ_CP144460
Coordinates   3463139..3464269 (-) Length   376 a.a.
NCBI ID   WP_005990431.1    Uniprot ID   Q8P759
Organism   Xanthomonas sp. 10-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3458139..3469269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZ068_RS14545 (VZ068_14550) - 3458655..3460511 (+) 1857 WP_349655697.1 DUF4153 domain-containing protein -
  VZ068_RS14550 (VZ068_14555) - 3460508..3461047 (+) 540 WP_259151738.1 DNA-3-methyladenine glycosylase I -
  VZ068_RS14560 (VZ068_14565) - 3461599..3462273 (-) 675 WP_349657717.1 YitT family protein -
  VZ068_RS14565 (VZ068_14570) - 3462546..3463127 (-) 582 WP_349655698.1 MFS transporter permease -
  VZ068_RS14570 (VZ068_14575) pilU 3463139..3464269 (-) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VZ068_RS14575 (VZ068_14580) pilT 3464391..3465428 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  VZ068_RS14580 (VZ068_14585) - 3465757..3466449 (+) 693 WP_349655699.1 YggS family pyridoxal phosphate-dependent enzyme -
  VZ068_RS14585 (VZ068_14590) proC 3466499..3467353 (+) 855 WP_349655700.1 pyrroline-5-carboxylate reductase -
  VZ068_RS14590 (VZ068_14595) - 3467875..3468294 (+) 420 WP_039404742.1 HU family DNA-binding protein -
  VZ068_RS14595 (VZ068_14600) - 3468426..3469043 (+) 618 WP_349655701.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41996.26 Da        Isoelectric Point: 6.8287

>NTDB_id=933957 VZ068_RS14570 WP_005990431.1 3463139..3464269(-) (pilU) [Xanthomonas sp. 10-10]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=933957 VZ068_RS14570 WP_005990431.1 3463139..3464269(-) (pilU) [Xanthomonas sp. 10-10]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATTACCTCCGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATCACGCAGACGCCATTGACCGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTCCGTGTCAGCTGTTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGCATCCC
TACGGTCGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGCAAGTCGACCTCGCTGGCAGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCATGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAATGCGCTGAAAAATACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAATCAGCTGCTGATGGATCTGTCGCTGAACCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCGACCCCGGATGGGCGCGGTCGCCGTGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGATTACATCCGCGATGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTCTATCAGGCTGGTGAAATCAGCTACGAAGACGCGCTGCGTTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGACGCCAAGACGCTGTCGCAGGGGCTTGATGGGGTGGAGATCGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367