Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VX159_RS01605 Genome accession   NZ_CP144212
Coordinates   329942..330985 (+) Length   347 a.a.
NCBI ID   WP_290898210.1    Uniprot ID   -
Organism   Dechloromonas sp. ZY10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 324942..335985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VX159_RS01580 (VX159_01580) - 325514..327508 (+) 1995 WP_371324236.1 polysaccharide biosynthesis protein -
  VX159_RS01585 (VX159_01585) - 327505..327795 (-) 291 WP_371324237.1 DUF167 domain-containing protein -
  VX159_RS01590 (VX159_01590) - 327802..328332 (-) 531 WP_371324238.1 YggT family protein -
  VX159_RS01595 (VX159_01595) proC 328334..329155 (-) 822 WP_371324239.1 pyrroline-5-carboxylate reductase -
  VX159_RS01600 (VX159_01600) - 329152..329847 (-) 696 WP_371324240.1 YggS family pyridoxal phosphate-dependent enzyme -
  VX159_RS01605 (VX159_01605) pilT 329942..330985 (+) 1044 WP_290898210.1 type IV pilus twitching motility protein PilT Machinery gene
  VX159_RS01610 (VX159_01610) pilU 331017..332153 (+) 1137 WP_371324241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VX159_RS01615 (VX159_01615) - 332185..332865 (+) 681 WP_371324242.1 DUF4197 domain-containing protein -
  VX159_RS01620 (VX159_01620) - 333027..333350 (+) 324 WP_371324243.1 hypothetical protein -
  VX159_RS01625 (VX159_01625) - 333436..335442 (+) 2007 WP_371324244.1 UvrD-helicase domain-containing protein -
  VX159_RS01630 (VX159_01630) - 335528..335974 (-) 447 WP_371324245.1 cytochrome c5 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38457.01 Da        Isoelectric Point: 6.9315

>NTDB_id=932609 VX159_RS01605 WP_290898210.1 329942..330985(+) (pilT) [Dechloromonas sp. ZY10]
MDITELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKEVHGMVYDIMNDAQRKHYEETLECDFSFEIPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKDISETPRGIVLVTGPTGSGKSTTLAAMVNHINENDYGHILTVED
PIEFVHESKRCLINQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGNSRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQGFGMQTLDQN
LLDLVRRNVVSSVEARSKAANKDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=932609 VX159_RS01605 WP_290898210.1 329942..330985(+) (pilT) [Dechloromonas sp. ZY10]
ATGGATATTACCGAACTGCTGGCTTTCAGCGTCAAGAACAAGGCTTCCGATTTGCACCTTTCGGCCGGGTTGCCGCCGAT
GATCCGGGTGCACGGCGACGTGCGCCGGATCAACCTGCCGCCGCTGGAGCACAAGGAAGTGCACGGCATGGTGTACGACA
TCATGAATGACGCCCAGCGCAAGCACTACGAAGAGACGCTGGAATGCGACTTCTCGTTTGAAATCCCCAACTTGGCGCGT
TTCCGGGTCAATGCCTTCAACCAGAATCGCGGTGCCGGCGCGGTGTTCCGGACCATTCCGTCGAAGGTCCTGACGCTGGA
GGAACTCAACTGCCCGAAAATCTTCAAGGATATTTCGGAAACCCCGCGCGGCATTGTGCTGGTCACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAACCACATCAACGAAAACGACTACGGCCACATCCTGACCGTTGAGGAT
CCGATCGAATTCGTGCATGAGTCCAAGCGCTGCCTGATCAACCAGCGCGAAGTCGGGCCGCATACGCTGTCGTTCAACAA
CGCCTTGCGCTCGGCCTTGCGCGAGGATCCGGACGTGATCCTGGTCGGTGAAATGCGCGACCTGGAAACCATTCGTCTGG
CCCTGTCGGCGGCCGAAACCGGTCACCTGGTGTTCGGTACCCTGCATACTTCGTCGGCGGCCAAGACGGTGGACCGGATC
GTCGATGTGTTCCCGGCGGCGGAGAAGGAAATGGTCCGTTCGATGCTCTCCGAGTCGCTGCGTGCGGTGATTTCGCAAAC
CCTGCTCAAGACCAAGGATGGCAACAGCCGGGTCGCGGCACACGAAATCATGATCGGCACCCCGGCGATCCGCAACCTGA
TCCGCGAAAACAAGGTGGCACAGATGTATTCGGCGATTCAGACCGGTCAGGGCTTCGGCATGCAGACCCTGGACCAGAAT
CTGCTCGACCTGGTCCGGCGCAATGTCGTCTCCAGCGTCGAGGCCCGCAGCAAGGCGGCCAACAAGGACAACTTCCCCGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.13

99.424

0.787
  pilT Acinetobacter baumannii D1279779

79.13

99.424

0.787
  pilT Acinetobacter baumannii strain A118

79.13

99.424

0.787
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Acinetobacter baylyi ADP1

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

72.464

99.424

0.72
  pilT Legionella pneumophila strain ERS1305867

72.464

99.424

0.72
  pilT Neisseria meningitidis 8013

72.174

99.424

0.718
  pilT Neisseria gonorrhoeae MS11

71.884

99.424

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.935

97.406

0.671
  pilT Vibrio cholerae strain A1552

68.935

97.406

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Vibrio cholerae strain A1552

40.29

99.424

0.401
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398