Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VX159_RS01610 Genome accession   NZ_CP144212
Coordinates   331017..332153 (+) Length   378 a.a.
NCBI ID   WP_371324241.1    Uniprot ID   -
Organism   Dechloromonas sp. ZY10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326017..337153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VX159_RS01585 (VX159_01585) - 327505..327795 (-) 291 WP_371324237.1 DUF167 domain-containing protein -
  VX159_RS01590 (VX159_01590) - 327802..328332 (-) 531 WP_371324238.1 YggT family protein -
  VX159_RS01595 (VX159_01595) proC 328334..329155 (-) 822 WP_371324239.1 pyrroline-5-carboxylate reductase -
  VX159_RS01600 (VX159_01600) - 329152..329847 (-) 696 WP_371324240.1 YggS family pyridoxal phosphate-dependent enzyme -
  VX159_RS01605 (VX159_01605) pilT 329942..330985 (+) 1044 WP_290898210.1 type IV pilus twitching motility protein PilT Machinery gene
  VX159_RS01610 (VX159_01610) pilU 331017..332153 (+) 1137 WP_371324241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VX159_RS01615 (VX159_01615) - 332185..332865 (+) 681 WP_371324242.1 DUF4197 domain-containing protein -
  VX159_RS01620 (VX159_01620) - 333027..333350 (+) 324 WP_371324243.1 hypothetical protein -
  VX159_RS01625 (VX159_01625) - 333436..335442 (+) 2007 WP_371324244.1 UvrD-helicase domain-containing protein -
  VX159_RS01630 (VX159_01630) - 335528..335974 (-) 447 WP_371324245.1 cytochrome c5 family protein -
  VX159_RS01635 (VX159_01635) mobA 336207..336785 (-) 579 WP_371324246.1 molybdenum cofactor guanylyltransferase MobA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42146.43 Da        Isoelectric Point: 6.3481

>NTDB_id=932610 VX159_RS01610 WP_371324241.1 331017..332153(+) (pilU) [Dechloromonas sp. ZY10]
MERDQAMKFMHDLLRLMAQKKGSDLFVTAGFPPAIKIDGRITPVSNQALSPQHTAELARSIMNDRQAAEFEASKECNFAI
SPAGIGRFRVNALVQQGRVAIVCRTINMSIPTLDELQLPPVLKDLAMTKRGLIIFVGGTGTGKSTSLAALVDYRNSNSYG
HIITIEDPIEYVHEHKNCIVTQREVGVDTDDWGPALKNTLRQAPDVILMGEVRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEERRQQLLMDLSLNLRAMISQRLLPKKDGKGRRAAIEVMLNSPLIADLIFKGDVHEIKEIMKKSRELG
MQTFDQALFDLYEAGEISYEDALRNADSLNDLRLQIKLHGKESQDRDLASGIGHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=932610 VX159_RS01610 WP_371324241.1 331017..332153(+) (pilU) [Dechloromonas sp. ZY10]
ATGGAACGCGATCAGGCGATGAAGTTCATGCATGACCTGCTGCGCCTGATGGCGCAGAAAAAGGGGTCGGATCTGTTTGT
GACTGCCGGCTTTCCCCCGGCGATCAAGATCGACGGGCGGATCACGCCGGTCTCGAATCAGGCGCTGAGTCCGCAGCACA
CCGCCGAGCTGGCGCGTTCGATCATGAACGATCGCCAGGCGGCCGAATTCGAAGCCAGCAAGGAGTGCAATTTCGCGATT
TCGCCCGCCGGGATCGGGCGTTTCCGGGTCAATGCACTGGTTCAGCAGGGGCGGGTGGCGATTGTCTGCCGTACCATCAA
CATGAGCATCCCGACTCTGGACGAACTGCAGTTGCCGCCGGTACTCAAGGATCTGGCGATGACCAAGCGCGGGCTGATCA
TCTTTGTCGGCGGTACCGGCACCGGCAAATCAACCTCGCTGGCGGCGCTGGTCGATTACCGTAACAGCAACTCCTATGGT
CACATCATCACCATCGAAGACCCGATCGAGTACGTGCACGAGCACAAGAACTGCATCGTCACCCAGCGCGAGGTCGGGGT
CGATACCGATGACTGGGGGCCGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGGTGCGCG
ACCGCGAAACCATGGATTACGCGATTGCCTTTGCCGAAACCGGCCACCTGTGTCTGGCGACCCTGCATGCGAACAGCGCC
AACCAGGCCATCGACCGGATCATCAATTTCTTCCCCGAGGAGCGCCGGCAGCAGTTGTTGATGGATCTGTCGCTCAACTT
GCGGGCGATGATTTCGCAGCGGCTGTTACCGAAAAAGGACGGCAAGGGGCGGCGGGCGGCGATTGAGGTGATGCTCAACT
CGCCCTTGATCGCTGACCTGATCTTCAAGGGCGATGTGCATGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGTTGGGC
ATGCAGACCTTCGATCAGGCCTTGTTCGACCTCTACGAGGCCGGTGAGATCAGCTATGAGGATGCGCTACGCAATGCCGA
TTCGCTCAATGACCTGCGCCTGCAGATCAAGCTGCATGGCAAGGAATCGCAAGACCGCGACCTGGCTTCCGGGATCGGTC
ATCTCGATATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.11

96.296

0.627
  pilU Acinetobacter baylyi ADP1

61.429

92.593

0.569
  pilU Vibrio cholerae strain A1552

55.425

90.212

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.179

88.624

0.392
  pilT Pseudomonas aeruginosa PAK

43.582

88.624

0.386
  pilT Acinetobacter baylyi ADP1

43.027

89.153

0.384
  pilT Legionella pneumophila strain ERS1305867

43.505

87.566

0.381
  pilT Legionella pneumophila strain Lp02

43.505

87.566

0.381
  pilT Pseudomonas stutzeri DSM 10701

42.985

88.624

0.381
  pilT Acinetobacter nosocomialis M2

42.598

87.566

0.373
  pilT Acinetobacter baumannii D1279779

42.598

87.566

0.373
  pilT Acinetobacter baumannii strain A118

42.598

87.566

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.492

83.333

0.362
  pilT Vibrio cholerae strain A1552

43.492

83.333

0.362