Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VUI23_RS04350 Genome accession   NZ_CP144164
Coordinates   1029929..1030972 (+) Length   347 a.a.
NCBI ID   WP_216046882.1    Uniprot ID   -
Organism   Alteromonas sp. M12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1024929..1035972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUI23_RS04325 - 1026017..1027300 (-) 1284 WP_342807000.1 extracellular solute-binding protein -
  VUI23_RS04330 - 1027401..1027826 (-) 426 WP_342807002.1 DUF4426 domain-containing protein -
  VUI23_RS04335 - 1027836..1028357 (-) 522 WP_216046885.1 YggT family protein -
  VUI23_RS04340 proC 1028372..1029193 (-) 822 WP_342807004.1 pyrroline-5-carboxylate reductase -
  VUI23_RS04345 - 1029207..1029899 (-) 693 WP_216046883.1 YggS family pyridoxal phosphate-dependent enzyme -
  VUI23_RS04350 pilT 1029929..1030972 (+) 1044 WP_216046882.1 type IV pilus twitching motility protein PilT Machinery gene
  VUI23_RS04355 pilU 1030982..1032094 (+) 1113 WP_216046881.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VUI23_RS04360 - 1032100..1032426 (+) 327 WP_216046880.1 DUF2007 domain-containing protein -
  VUI23_RS04365 ruvX 1032429..1032848 (-) 420 WP_216046879.1 Holliday junction resolvase RuvX -
  VUI23_RS04370 - 1032864..1033421 (-) 558 WP_216046878.1 YqgE/AlgH family protein -
  VUI23_RS04375 gshB 1033408..1034373 (-) 966 WP_303500534.1 glutathione synthase -
  VUI23_RS04380 cysK 1034566..1035546 (-) 981 WP_216046957.1 cysteine synthase A -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38440.22 Da        Isoelectric Point: 6.7807

>NTDB_id=932543 VUI23_RS04350 WP_216046882.1 1029929..1030972(+) (pilT) [Alteromonas sp. M12]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEIRRLNVPELDHKQVHALVYEIMNDKQRKEYEENLEVDFSFEVKDLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPAIFKDIINQPTGIVLVTGATGSGKSTTLAAMIDHINTSKREHILTIED
PIEFVHQNKLCVLNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFATLHTNSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMIGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAQGVITAQDAASKSVDKQPKIGF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=932543 VUI23_RS04350 WP_216046882.1 1029929..1030972(+) (pilT) [Alteromonas sp. M12]
TTGGATATTACCGAACTTTTAGCTTTTAGTGTTAAGAATAATGCATCAGATTTACATCTATCAGCGGGGTTGCCACCGAT
AATACGTGTAGACGGTGAAATTCGCCGTTTAAATGTGCCGGAATTGGATCATAAACAGGTACATGCGTTGGTTTACGAAA
TCATGAACGATAAACAACGTAAAGAATACGAAGAAAATTTGGAAGTGGATTTTTCTTTTGAAGTAAAAGATTTATCTCGA
TTTCGTGTCAATGCCTTTGTGCAAAACCGAGGCGCAGCGGCAGTACTAAGGACTATCCCGAGTAAAGTCCTCACTTTGGA
TGATTTGGGCGCTCCGGCTATTTTCAAAGATATTATCAATCAGCCTACAGGTATAGTGTTGGTTACCGGGGCAACAGGTT
CGGGGAAAAGTACGACCTTGGCGGCGATGATAGATCACATCAATACTAGTAAAAGAGAGCACATTCTAACCATTGAAGAT
CCCATTGAATTTGTCCATCAAAACAAACTTTGTGTACTCAACCAACGTGAAGTGCATCGGGATACTCACAGTTTTAATAA
TGCGCTGCGATCTGCATTACGGGAAGATCCCGATGTGATACTGGTGGGGGAATTACGGGACTTAGAAACCATTCGCTTGG
CGATCTCCGCTGCCGAAACAGGCCATTTGGTCTTTGCAACTTTACATACCAACTCAGCGCCTAAGACCATAGATAGAATC
ATTGACGTATTTCCGGCTGAAGAAAAGTCGATGGTACGCTCCATGCTCTCAGAGTCATTGCGAGCGGTTATTTCTCAAAC
ATTGTTGAAGAAGGTGGGCGGCGGCCGAATTGCAGCTCATGAGATTATGATAGGAATTCCAGCAATTCGTAATTTGATCC
GTGAAGATAAGGTTCCGCAAATGTATTCGGTAATTCAAACCGGTCAGGCCCATGGAATGCAAACCATGGATCAATGTTTA
CAACGTCTCGTTGCTCAAGGCGTGATAACCGCCCAAGATGCGGCGTCTAAATCTGTTGATAAACAACCAAAAATTGGTTT
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

76.744

99.135

0.761
  pilT Acinetobacter baumannii D1279779

76.744

99.135

0.761
  pilT Acinetobacter nosocomialis M2

76.453

99.135

0.758
  pilT Pseudomonas stutzeri DSM 10701

74.419

99.135

0.738
  pilT Acinetobacter baylyi ADP1

73.837

99.135

0.732
  pilT Pseudomonas aeruginosa PAK

73.964

97.406

0.72
  pilT Legionella pneumophila strain Lp02

70.968

98.271

0.697
  pilT Legionella pneumophila strain ERS1305867

70.968

98.271

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.513

97.118

0.695
  pilT Vibrio cholerae strain A1552

71.513

97.118

0.695
  pilT Neisseria gonorrhoeae MS11

65.23

100

0.654
  pilT Neisseria meningitidis 8013

65.598

98.847

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.895

98.847

0.513
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

42.262

96.83

0.409
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403