Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VUI23_RS04355 Genome accession   NZ_CP144164
Coordinates   1030982..1032094 (+) Length   370 a.a.
NCBI ID   WP_216046881.1    Uniprot ID   -
Organism   Alteromonas sp. M12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1025982..1037094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUI23_RS04325 - 1026017..1027300 (-) 1284 WP_342807000.1 extracellular solute-binding protein -
  VUI23_RS04330 - 1027401..1027826 (-) 426 WP_342807002.1 DUF4426 domain-containing protein -
  VUI23_RS04335 - 1027836..1028357 (-) 522 WP_216046885.1 YggT family protein -
  VUI23_RS04340 proC 1028372..1029193 (-) 822 WP_342807004.1 pyrroline-5-carboxylate reductase -
  VUI23_RS04345 - 1029207..1029899 (-) 693 WP_216046883.1 YggS family pyridoxal phosphate-dependent enzyme -
  VUI23_RS04350 pilT 1029929..1030972 (+) 1044 WP_216046882.1 type IV pilus twitching motility protein PilT Machinery gene
  VUI23_RS04355 pilU 1030982..1032094 (+) 1113 WP_216046881.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VUI23_RS04360 - 1032100..1032426 (+) 327 WP_216046880.1 DUF2007 domain-containing protein -
  VUI23_RS04365 ruvX 1032429..1032848 (-) 420 WP_216046879.1 Holliday junction resolvase RuvX -
  VUI23_RS04370 - 1032864..1033421 (-) 558 WP_216046878.1 YqgE/AlgH family protein -
  VUI23_RS04375 gshB 1033408..1034373 (-) 966 WP_303500534.1 glutathione synthase -
  VUI23_RS04380 cysK 1034566..1035546 (-) 981 WP_216046957.1 cysteine synthase A -
  VUI23_RS04385 - 1035670..1036122 (+) 453 WP_303500535.1 Lrp/AsnC family transcriptional regulator -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41071.14 Da        Isoelectric Point: 5.4382

>NTDB_id=932544 VUI23_RS04355 WP_216046881.1 1030982..1032094(+) (pilU) [Alteromonas sp. M12]
MDLNPYLRKMAEQGASDLFITVGFPISAKINGQMTPINDERLSEEAALALVHNVMSEKQQDEFNNSKECNFAIARDGIGR
FRCSAFWQRDMAGMVVRRIVTQIPKADELGLPEILKDVIMSKRGLLLFVGATGTGKSTSLAALMGHRNQNSRGHILTIED
PIEFVHEHAGCVVTQREVGIDTRSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERI
MHLAPPEQHEKLRFDLSLNLRAIVAQQLVPTIDGKGRVAAIEILLNSPLVTDLIQRNEIGSLKEAMKKGKEMGMQSFDMA
LYELYKQERISMEQAIHHADSPNDLRLMIKLDTEEGTGLGSLSNVSIDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=932544 VUI23_RS04355 WP_216046881.1 1030982..1032094(+) (pilU) [Alteromonas sp. M12]
ATGGACTTAAATCCTTATCTGAGAAAAATGGCAGAACAAGGTGCATCAGACTTGTTTATTACAGTTGGCTTTCCCATTAG
TGCAAAAATAAATGGGCAAATGACGCCGATCAACGATGAGCGTTTAAGTGAAGAAGCCGCACTTGCACTGGTTCATAACG
TTATGAGCGAGAAACAGCAGGACGAATTTAATAACAGTAAAGAGTGTAACTTTGCGATTGCTAGAGATGGCATTGGACGT
TTTCGTTGCAGTGCCTTTTGGCAGCGGGATATGGCCGGCATGGTGGTTCGCCGCATCGTTACGCAAATTCCCAAAGCCGA
TGAGCTAGGCCTACCAGAGATACTTAAAGACGTCATAATGTCCAAGCGAGGACTGTTGTTGTTTGTTGGTGCTACAGGTA
CCGGTAAATCAACATCCTTAGCTGCATTGATGGGACATCGTAATCAAAACTCAAGAGGCCACATATTGACCATTGAGGAC
CCTATCGAATTTGTCCATGAACATGCAGGCTGTGTGGTGACACAACGGGAAGTGGGCATAGACACTCGTTCGTTCGACGA
TGCGCTTAAAAGTTCATTGCGACAAGCGCCGGATGTGATTTTGATTGGCGAAATTCGATCTATGGAAACCATGGAATATG
CCATGTCATTTGCCGATACCGGGCACTTATGTGTGGCGACTTTACACGCCAACAATGCCAATCAGGCGATTGAACGTATT
ATGCACTTAGCACCACCAGAGCAGCATGAAAAATTGCGTTTCGATTTGAGTTTAAATTTGCGAGCCATTGTTGCACAACA
GCTTGTTCCTACGATTGACGGTAAAGGGCGGGTTGCGGCCATCGAAATTTTACTTAACTCGCCATTAGTGACAGATTTAA
TTCAGCGCAATGAAATTGGTTCGCTCAAAGAAGCAATGAAAAAAGGCAAAGAAATGGGGATGCAAAGCTTTGATATGGCT
TTGTATGAATTATATAAACAAGAACGCATTAGCATGGAACAGGCTATCCATCATGCTGATTCACCAAATGACCTGCGTTT
AATGATTAAACTGGATACGGAAGAGGGGACAGGTTTAGGTTCACTGTCTAATGTTTCCATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.714

98.378

0.597
  pilU Vibrio cholerae strain A1552

58.108

100

0.581
  pilU Acinetobacter baylyi ADP1

57.493

99.189

0.57
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.598

98.108

0.408
  pilT Pseudomonas aeruginosa PAK

40.87

93.243

0.381
  pilT Pseudomonas stutzeri DSM 10701

38.841

93.243

0.362
  pilT Legionella pneumophila strain Lp02

40

90.541

0.362
  pilT Legionella pneumophila strain ERS1305867

40

90.541

0.362