Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VWP12_RS00785 Genome accession   NZ_CP144142
Coordinates   166619..167668 (+) Length   349 a.a.
NCBI ID   WP_125199336.1    Uniprot ID   A0A426QNH7
Organism   Guyparkeria sp. TX1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 161619..172668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VWP12_RS00755 (VWP12_00755) - 161810..162409 (+) 600 WP_410474211.1 class I SAM-dependent methyltransferase -
  VWP12_RS00760 (VWP12_00760) metW 162446..163051 (-) 606 WP_410276672.1 methionine biosynthesis protein MetW -
  VWP12_RS00765 (VWP12_00765) - 163048..164241 (-) 1194 WP_410474212.1 homoserine O-acetyltransferase -
  VWP12_RS00770 (VWP12_00770) - 164273..164824 (-) 552 WP_410474213.1 YggT family protein -
  VWP12_RS00775 (VWP12_00775) proC 164910..165749 (-) 840 WP_410474214.1 pyrroline-5-carboxylate reductase -
  VWP12_RS00780 (VWP12_00780) - 165825..166487 (-) 663 WP_410474215.1 YggS family pyridoxal phosphate-dependent enzyme -
  VWP12_RS00785 (VWP12_00785) pilT 166619..167668 (+) 1050 WP_125199336.1 type IV pilus twitching motility protein PilT Machinery gene
  VWP12_RS00790 (VWP12_00790) pilU 167810..168955 (+) 1146 WP_125199335.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VWP12_RS00795 (VWP12_00795) - 169049..170203 (+) 1155 WP_410474216.1 PilT/PilU family type 4a pilus ATPase -
  VWP12_RS00805 (VWP12_00805) thiO 170674..171750 (+) 1077 WP_410474217.1 glycine oxidase ThiO -
  VWP12_RS00810 (VWP12_00810) - 171786..172124 (-) 339 WP_125199332.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38996.37 Da        Isoelectric Point: 7.1150

>NTDB_id=932488 VWP12_RS00785 WP_125199336.1 166619..167668(+) (pilT) [Guyparkeria sp. TX1]
MAESRDIDSLLMFTVKNGASDLHLSAGEQPRVRIDGDIRKVNVPVMDHKDVHSMVYDIMNDRQRKEYEDNLEVDFSFELR
GHARFRVNAFNQNRGASAVFRTIPSKVLTLEQLDAPKVFQDLCDYPNGIVLVTGPTGSGKSTTLAAMIDYVNDHRNDHIL
TVEDPIEFVHESKKCLVNQREVHRDTHSFNAALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTNSAAKT
IDRIIDVFPSAEKDMVRAMLSESLRAVISQTLLKKISGGRVAAHEIMLGTPAIRNLIRENKVAQMYSAIQTGQAQGMQTL
DQNLKQLVSRGAVSKELARGRAKNKDDFR

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=932488 VWP12_RS00785 WP_125199336.1 166619..167668(+) (pilT) [Guyparkeria sp. TX1]
ATGGCCGAATCCCGAGATATCGACTCCCTGCTGATGTTCACCGTCAAGAACGGGGCATCGGACCTCCACCTCTCGGCCGG
CGAGCAGCCCCGGGTGCGGATCGACGGCGACATCCGCAAGGTCAACGTGCCGGTAATGGACCACAAGGATGTCCATTCCA
TGGTGTACGACATCATGAACGACCGGCAGCGCAAGGAGTACGAAGACAACCTCGAGGTCGACTTCTCCTTCGAACTGCGC
GGCCATGCCCGCTTCCGTGTCAACGCCTTTAACCAGAACCGCGGCGCGAGTGCGGTCTTCCGCACCATCCCGAGCAAGGT
GCTCACCCTAGAGCAGCTCGACGCGCCGAAGGTCTTCCAGGACCTGTGCGACTACCCCAACGGCATCGTCCTGGTGACCG
GCCCGACCGGCTCGGGCAAGTCGACCACGCTCGCGGCGATGATCGACTACGTCAACGACCATCGCAACGACCATATCCTC
ACGGTCGAGGATCCGATCGAATTCGTCCACGAATCGAAAAAATGCCTCGTCAACCAGCGCGAGGTCCATCGCGACACCCA
CAGCTTCAACGCCGCATTGCGCTCGGCGCTGCGCGAAGACCCGGACGTGGTCCTGGTCGGCGAACTGCGTGATCTCGAAA
CCATCCGCCTGGCCCTGACCGCCGCGGAGACCGGCCACCTGGTGTTCGGCACGCTACACACCAACTCGGCGGCCAAAACC
ATCGACCGGATCATCGACGTCTTCCCGTCGGCGGAGAAAGACATGGTGCGCGCGATGCTCTCGGAGTCCCTGCGCGCGGT
CATTTCGCAGACCCTGCTCAAGAAGATCAGCGGCGGGCGCGTCGCGGCCCACGAGATCATGCTGGGCACGCCGGCGATCC
GCAACCTGATCCGCGAGAACAAGGTCGCGCAAATGTACTCCGCGATCCAGACCGGCCAGGCACAAGGGATGCAAACGCTC
GATCAGAACCTCAAGCAGCTGGTCTCGCGCGGCGCGGTTTCCAAGGAACTCGCGCGCGGCCGGGCAAAGAACAAGGACGA
CTTCAGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A426QNH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.259

98.281

0.759
  pilT Acinetobacter baumannii D1279779

76.968

98.281

0.756
  pilT Acinetobacter baumannii strain A118

76.968

98.281

0.756
  pilT Acinetobacter baylyi ADP1

74.636

98.281

0.734
  pilT Pseudomonas aeruginosa PAK

73.469

98.281

0.722
  pilT Pseudomonas stutzeri DSM 10701

72.886

98.281

0.716
  pilT Legionella pneumophila strain Lp02

71.429

98.281

0.702
  pilT Legionella pneumophila strain ERS1305867

71.429

98.281

0.702
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.39

93.983

0.699
  pilT Vibrio cholerae strain A1552

74.39

93.983

0.699
  pilT Neisseria meningitidis 8013

66.181

98.281

0.65
  pilT Neisseria gonorrhoeae MS11

65.889

98.281

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.711

100

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.159

98.854

0.407
  pilU Vibrio cholerae strain A1552

42.47

95.129

0.404
  pilU Acinetobacter baylyi ADP1

38.728

99.14

0.384