Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VWP12_RS00790 Genome accession   NZ_CP144142
Coordinates   167810..168955 (+) Length   381 a.a.
NCBI ID   WP_125199335.1    Uniprot ID   A0A426QNG0
Organism   Guyparkeria sp. TX1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 162810..173955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VWP12_RS00765 (VWP12_00765) - 163048..164241 (-) 1194 WP_410474212.1 homoserine O-acetyltransferase -
  VWP12_RS00770 (VWP12_00770) - 164273..164824 (-) 552 WP_410474213.1 YggT family protein -
  VWP12_RS00775 (VWP12_00775) proC 164910..165749 (-) 840 WP_410474214.1 pyrroline-5-carboxylate reductase -
  VWP12_RS00780 (VWP12_00780) - 165825..166487 (-) 663 WP_410474215.1 YggS family pyridoxal phosphate-dependent enzyme -
  VWP12_RS00785 (VWP12_00785) pilT 166619..167668 (+) 1050 WP_125199336.1 type IV pilus twitching motility protein PilT Machinery gene
  VWP12_RS00790 (VWP12_00790) pilU 167810..168955 (+) 1146 WP_125199335.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VWP12_RS00795 (VWP12_00795) - 169049..170203 (+) 1155 WP_410474216.1 PilT/PilU family type 4a pilus ATPase -
  VWP12_RS00805 (VWP12_00805) thiO 170674..171750 (+) 1077 WP_410474217.1 glycine oxidase ThiO -
  VWP12_RS00810 (VWP12_00810) - 171786..172124 (-) 339 WP_125199332.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42549.45 Da        Isoelectric Point: 5.3306

>NTDB_id=932489 VWP12_RS00790 WP_125199335.1 167810..168955(+) (pilU) [Guyparkeria sp. TX1]
MDRQQAANYVQALLKRAVERDASDLFVTVGAPPSAKIDGKVVHLTDQKLSVENTQMLVRSIMTDKQVAHFEESHEANFAI
ALPGIARFRVNAFMQRGSEGMVLRMIRSDIPNFDDLKLPDVLGEIAMTARGLVIFVGATSSGKSTSLAAMLDYRNENSRG
HIVTIEDPIEFVHKHKGCLVTQREVGVDTESFAAALKNTLRQAPDVILIGEIRDRETMDHAIAFAETGHLCLATLHANNT
NQALDRIINFFPEERRQQLLMDLSLNLKSVISQRLLRKADGEGRVPAVEVLINTPLIGDLIFKGDVSEIKEVMKRSRELG
MQTFDQALFDLYDSHQADYDEVISKADSVNDLRLRIKLESNRGLPKSRQDDSEDDLKVEAD

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=932489 VWP12_RS00790 WP_125199335.1 167810..168955(+) (pilU) [Guyparkeria sp. TX1]
ATGGACCGTCAGCAAGCAGCCAACTACGTACAAGCCCTCCTCAAACGGGCCGTTGAACGCGACGCCTCCGACCTGTTCGT
CACCGTCGGCGCACCGCCCTCGGCGAAGATCGACGGCAAGGTCGTCCACCTGACCGATCAGAAGCTCTCGGTGGAAAACA
CCCAGATGCTGGTCCGCTCGATCATGACGGACAAGCAGGTGGCCCATTTCGAGGAATCCCACGAGGCGAACTTCGCCATC
GCCCTGCCGGGGATAGCGCGCTTTCGTGTCAATGCGTTCATGCAGCGTGGCAGCGAGGGTATGGTCCTGCGGATGATCCG
CTCGGACATCCCCAACTTCGACGACCTCAAGCTCCCCGACGTGCTAGGCGAGATCGCGATGACCGCACGGGGCCTGGTGA
TCTTCGTCGGCGCCACCAGCTCGGGCAAGTCCACGTCGTTGGCGGCGATGCTCGACTATCGCAACGAAAATTCGCGTGGG
CACATCGTCACCATCGAGGACCCGATCGAGTTCGTCCACAAGCACAAGGGCTGTCTTGTCACCCAGCGCGAGGTGGGCGT
GGATACCGAATCCTTCGCCGCGGCACTCAAGAACACCCTGCGCCAGGCCCCCGACGTGATCCTGATCGGCGAGATCCGCG
ACCGCGAGACCATGGACCATGCCATTGCCTTCGCCGAGACTGGCCACCTGTGCCTGGCCACGCTGCACGCGAACAACACC
AACCAGGCACTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGGCGTCAGCAGTTGTTGATGGACCTGTCGCTCAACCT
CAAGTCGGTCATCTCGCAACGCTTGTTGCGCAAGGCGGATGGCGAGGGCCGGGTTCCGGCCGTCGAGGTACTGATCAACA
CCCCCCTGATCGGCGACCTGATCTTCAAGGGCGATGTCTCCGAGATCAAGGAAGTGATGAAGCGCTCGCGCGAACTGGGC
ATGCAGACATTCGACCAGGCCCTGTTCGACCTCTACGACAGCCACCAGGCCGACTACGACGAGGTCATCAGCAAGGCCGA
TTCCGTCAACGACCTGCGTCTGCGCATCAAGCTGGAAAGCAATCGCGGCCTGCCCAAGAGCCGCCAGGACGACTCCGAAG
ACGATCTCAAAGTCGAGGCCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A426QNG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.857

91.864

0.577
  pilU Acinetobacter baylyi ADP1

58.333

97.638

0.57
  pilU Vibrio cholerae strain A1552

56.14

89.764

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.91

87.664

0.394
  pilT Pseudomonas stutzeri DSM 10701

45.141

83.727

0.378
  pilT Acinetobacter baumannii strain A118

44.72

84.514

0.378
  pilT Acinetobacter nosocomialis M2

44.72

84.514

0.378
  pilT Acinetobacter baumannii D1279779

44.72

84.514

0.378
  pilT Pseudomonas aeruginosa PAK

44.72

84.514

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.262

88.189

0.373
  pilT Vibrio cholerae strain A1552

42.262

88.189

0.373
  pilT Acinetobacter baylyi ADP1

43.789

84.514

0.37
  pilT Legionella pneumophila strain Lp02

42.593

85.039

0.362
  pilT Legionella pneumophila strain ERS1305867

42.593

85.039

0.362