Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q1I81_RS15140 Genome accession   NZ_CP143635
Coordinates   3431841..3432878 (-) Length   345 a.a.
NCBI ID   WP_037420775.1    Uniprot ID   A0A501XE78
Organism   Shewanella xiamenensis strain 8M38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3426841..3437878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q1I81_RS15115 ruvX 3427044..3427469 (+) 426 WP_037420762.1 Holliday junction resolvase RuvX -
  Q1I81_RS15120 - 3427517..3428839 (-) 1323 WP_330148872.1 IS4-like element ISSba6 family transposase -
  Q1I81_RS15125 hemH 3428962..3429945 (-) 984 WP_211164613.1 ferrochelatase -
  Q1I81_RS15130 - 3430014..3430544 (-) 531 WP_374899544.1 glutathione peroxidase -
  Q1I81_RS15135 pilU 3430719..3431831 (-) 1113 WP_188870156.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q1I81_RS15140 pilT 3431841..3432878 (-) 1038 WP_037420775.1 type IV pilus twitching motility protein PilT Machinery gene
  Q1I81_RS15145 - 3432914..3433633 (+) 720 WP_282633783.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q1I81_RS15150 proC 3433849..3434667 (+) 819 WP_330148873.1 pyrroline-5-carboxylate reductase -
  Q1I81_RS15155 - 3434742..3435290 (+) 549 WP_037420784.1 YggT family protein -
  Q1I81_RS15160 yggU 3435290..3435580 (+) 291 WP_172591409.1 DUF167 family protein YggU -
  Q1I81_RS15165 - 3435654..3436088 (+) 435 WP_037420790.1 DUF4426 domain-containing protein -
  Q1I81_RS15170 rdgB 3436203..3436820 (+) 618 WP_037420793.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38278.98 Da        Isoelectric Point: 6.9096

>NTDB_id=930109 Q1I81_RS15140 WP_037420775.1 3431841..3432878(-) (pilT) [Shewanella xiamenensis strain 8M38]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHPNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=930109 Q1I81_RS15140 WP_037420775.1 3431841..3432878(-) (pilT) [Shewanella xiamenensis strain 8M38]
ATGGAAATCACTGAGTTATTAGCTTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATCAATCTGCCCGCGCTCGATCATCAGGGTGTACACAGCCTTGTTTATGACA
TTATGAATGATAAACAACGCAAGGACTTTGAAGAACATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTGAACGCCTTTAACCAATCTCGCGGCGCTGCGGCGGTATTTCGTACCATTCCCAGTGATATCTTGTCACTTGA
GCAGCTAGGTGCGCCTGAAATTTTTAAAAAAATTGCCAGTTTTCCCCGTGGGTTAGTGTTAGTCACTGGGCCTACAGGTT
CGGGTAAGAGTACCACACTTGCGGCCATGGTCGATTACATCAATGAAAACCGCCATGATCATATCTTAACCATTGAAGAC
CCAATCGAATTTGTTCATCCCAACAAGCAATGTTTGATTAACCAACGGGAAGTACATCGCCATACCCACAGCTTTAACGC
GGCACTGCGTAGCGCACTGCGTGAAGACCCTGACGTTATCCTCGTGGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CGATGACTGCGGCTGAAACGGGTCACTTAGTTTTTGGTACCTTACACACCACGTCGGCGGCTAAGACTATTGACCGTGTG
GTTGACGTTTTCCCTGCCGGTGAAAAGGACATGGTTCGTACCATGTTATCTGAATCATTACAGGCGGTTATTTCACAAAC
CCTGATTAAGAAAATCGGTGGCGGCCGTGTGGCTGCCCACGAAATTATGATGGGTACGCCCGCTATTCGTAACCTTATCC
GTGAAGATAAAGTGGCGCAGATGTACTCAGCCATTCAAACGGGGATGGCCCATGGCATGCAAACACTCGAACAGTGTCTG
CAAAACTTAGTGAACCGTGGTCTCATCACCCGTGAGGATGCCATGGCGAAGAGCTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A501XE78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Acinetobacter nosocomialis M2

80.117

99.13

0.794
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.691

99.42

0.414
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391