Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q1I81_RS15135 Genome accession   NZ_CP143635
Coordinates   3430719..3431831 (-) Length   370 a.a.
NCBI ID   WP_188870156.1    Uniprot ID   -
Organism   Shewanella xiamenensis strain 8M38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3425719..3436831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q1I81_RS15105 yciH 3426053..3426382 (+) 330 WP_047533731.1 stress response translation initiation inhibitor YciH -
  Q1I81_RS15110 - 3426451..3427014 (+) 564 WP_037420759.1 YqgE/AlgH family protein -
  Q1I81_RS15115 ruvX 3427044..3427469 (+) 426 WP_037420762.1 Holliday junction resolvase RuvX -
  Q1I81_RS15120 - 3427517..3428839 (-) 1323 WP_330148872.1 IS4-like element ISSba6 family transposase -
  Q1I81_RS15125 hemH 3428962..3429945 (-) 984 WP_211164613.1 ferrochelatase -
  Q1I81_RS15130 - 3430014..3430544 (-) 531 WP_374899544.1 glutathione peroxidase -
  Q1I81_RS15135 pilU 3430719..3431831 (-) 1113 WP_188870156.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q1I81_RS15140 pilT 3431841..3432878 (-) 1038 WP_037420775.1 type IV pilus twitching motility protein PilT Machinery gene
  Q1I81_RS15145 - 3432914..3433633 (+) 720 WP_282633783.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q1I81_RS15150 proC 3433849..3434667 (+) 819 WP_330148873.1 pyrroline-5-carboxylate reductase -
  Q1I81_RS15155 - 3434742..3435290 (+) 549 WP_037420784.1 YggT family protein -
  Q1I81_RS15160 yggU 3435290..3435580 (+) 291 WP_172591409.1 DUF167 family protein YggU -
  Q1I81_RS15165 - 3435654..3436088 (+) 435 WP_037420790.1 DUF4426 domain-containing protein -
  Q1I81_RS15170 rdgB 3436203..3436820 (+) 618 WP_037420793.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41117.33 Da        Isoelectric Point: 6.3725

>NTDB_id=930108 Q1I81_RS15135 WP_188870156.1 3430719..3431831(-) (pilU) [Shewanella xiamenensis strain 8M38]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESVMTEAQKKEFHTTRECNFAFAVKELGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVAKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLQLYVDGEISYADALHHADSPNDLRLMIKLQNKEPASSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=930108 Q1I81_RS15135 WP_188870156.1 3430719..3431831(-) (pilU) [Shewanella xiamenensis strain 8M38]
ATGGATGTCCGTCCGTTTTTAAAAGTCATGGTGGAGCGTAAAGCCTCGGACCTGTTTATTACCGCAGGGTTTCCGCCGAG
CGCCAAAATCGATGGTGAGTTACGCCCGTTAGCCGAGAGTGCCTTCACGCCCGCGCAGTCTTTGGATTTTGTCGAGTCCG
TGATGACGGAGGCGCAAAAGAAGGAGTTTCACACGACTCGCGAGTGTAACTTTGCTTTTGCGGTTAAGGAGTTGGGGCGT
TTTCGCGTCAGCGCATTCTGGCAGCGTGAGTCTCCGGGTTGTGTTATGCGCCGGATTGAGACGAAAATCCCTGAAGTGGA
TGATTTAAAACTGCCACCCATTTTAAAAGATCTGGTGATGAGTAAGCGTGGACTTATCATCATGGTGGGGGGGACAGGGA
CCGGTAAATCGACTTCCTTAGCAGCATTAGTCGGTTACCGCAATGCCCATGCCCGCGGGCATATCTTGACGATTGAAGAC
CCTGTGGAATTTGTGCACGATCATCGCAAAAGTATCATTACCCAACGTGAAGTGGGCATAGACACTGAGTCCTTTGATGC
GGCGCTGAAGAGCTCGCTGCGTCAGGCGCCTGATGTGATCCTTATCGGTGAAATTCGAACCCAAGAAACCATGGAGTTTG
CGCTCTCCTTTGCTGAAACGGGCCATCTGTGTATGGCGACACTGCATGCTAACAATGCTAACCAAGCATTAGACCGAATT
ATGCACTTAGTGCCAGAAAGTAAACATCAACAGCTATTATTCGATTTGTCACTCAACCTGCGAGGTATTGTGGCGCAGCA
GCTGGTGGCTAAAGTTGATGGAACAGGGCGCCGGGCGGCGATTGAAGTCTTGATCAATACGCCACGTGTCGCGAGTTTGA
TTGCTAAAAACGAGCTGCATTTGCTCAAAGAAACCATGGCCAAATCGAATGAGCAGGGCATGCAGACCTTTGACCAAGCA
CTGTTACAGCTCTATGTCGATGGGGAGATCAGTTATGCCGACGCGCTTCACCATGCTGACTCACCCAACGACTTGCGGTT
AATGATTAAATTGCAAAATAAAGAACCCGCCAGTTCGAGCTTTATGGAAGGCGTGACCTTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.108

100

0.603
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.293

88.649

0.384
  pilT Vibrio cholerae strain A1552

43.293

88.649

0.384
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.29

93.243

0.376
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Legionella pneumophila strain Lp02

40.785

89.459

0.365
  pilT Legionella pneumophila strain ERS1305867

40.785

89.459

0.365
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362