Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q1I82_RS14505 Genome accession   NZ_CP143633
Coordinates   3349271..3350308 (-) Length   345 a.a.
NCBI ID   WP_037420775.1    Uniprot ID   A0A501XE78
Organism   Shewanella xiamenensis strain 8M34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3344271..3355308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q1I82_RS14470 - 3344435..3344749 (+) 315 WP_330130877.1 hypothetical protein -
  Q1I82_RS14475 yciH 3344912..3345241 (+) 330 WP_037420757.1 stress response translation initiation inhibitor YciH -
  Q1I82_RS14480 - 3345310..3345873 (+) 564 WP_069453279.1 YqgE/AlgH family protein -
  Q1I82_RS14485 ruvX 3345903..3346328 (+) 426 WP_037420762.1 Holliday junction resolvase RuvX -
  Q1I82_RS14490 hemH 3346392..3347375 (-) 984 WP_240293187.1 ferrochelatase -
  Q1I82_RS14495 - 3347444..3347977 (-) 534 WP_249553409.1 glutathione peroxidase -
  Q1I82_RS14500 pilU 3348149..3349261 (-) 1113 WP_188870156.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q1I82_RS14505 pilT 3349271..3350308 (-) 1038 WP_037420775.1 type IV pilus twitching motility protein PilT Machinery gene
  Q1I82_RS14510 - 3350344..3351057 (+) 714 WP_144375261.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q1I82_RS14515 proC 3351281..3352099 (+) 819 WP_144375262.1 pyrroline-5-carboxylate reductase -
  Q1I82_RS14520 - 3352174..3352722 (+) 549 WP_037420784.1 YggT family protein -
  Q1I82_RS14525 yggU 3352722..3353012 (+) 291 WP_144375264.1 DUF167 family protein YggU -
  Q1I82_RS14530 - 3353086..3353520 (+) 435 WP_037420790.1 DUF4426 domain-containing protein -
  Q1I82_RS14535 rdgB 3353635..3354252 (+) 618 WP_144375268.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38278.98 Da        Isoelectric Point: 6.9096

>NTDB_id=930088 Q1I82_RS14505 WP_037420775.1 3349271..3350308(-) (pilT) [Shewanella xiamenensis strain 8M34]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHPNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=930088 Q1I82_RS14505 WP_037420775.1 3349271..3350308(-) (pilT) [Shewanella xiamenensis strain 8M34]
ATGGAAATCACTGAGTTATTAGCTTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATCAATCTGCCCGCGCTCGATCATCAGGGTGTACACAGCCTTGTTTATGACA
TTATGAATGATAAACAACGCAAGGACTTTGAAGAACATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTGAACGCCTTTAACCAATCTCGCGGCGCTGCGGCGGTATTTCGTACCATTCCCAGTGATATCTTGTCACTTGA
GCAGTTAGGTGCGCCTGAAATTTTTAAAAAAATTGCCAGTTTTCCCCGTGGGTTAGTGTTAGTCACTGGGCCTACAGGTT
CCGGTAAGAGTACCACACTTGCGGCCATGGTCGATTACATCAATGAAAACCGCCATGATCATATCTTAACCATTGAAGAC
CCAATCGAATTTGTTCATCCCAACAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGTCATACCCACAGCTTTAACGC
GGCGCTGCGTAGCGCACTGCGTGAAGACCCTGACGTTATCCTCGTGGGTGAGATGCGCGACCTTGAAACCATTCGTCTGG
CGATGACGGCGGCCGAAACGGGTCACTTAGTCTTTGGTACCTTGCACACCACCTCGGCGGCTAAGACCATCGACCGTGTG
GTTGACGTTTTCCCTGCCGGTGAAAAGGACATGGTGCGTACCATGTTATCTGAATCATTACAGGCGGTTATTTCGCAAAC
CCTGATTAAGAAAATCGGTGGCGGCCGTGTGGCAGCCCACGAAATTATGATGGGTACGCCCGCTATCCGAAATCTTATCC
GTGAAGATAAAGTGGCGCAGATGTACTCTGCCATTCAAACGGGGATGGCCCATGGCATGCAAACTCTTGAGCAGTGTCTG
CAAAACTTAGTTAACCGTGGCCTCATCACCCGTGAGGATGCCATGGCGAAGAGCTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A501XE78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Acinetobacter nosocomialis M2

80.117

99.13

0.794
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.691

99.42

0.414
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391