Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q1I82_RS14500 Genome accession   NZ_CP143633
Coordinates   3348149..3349261 (-) Length   370 a.a.
NCBI ID   WP_188870156.1    Uniprot ID   -
Organism   Shewanella xiamenensis strain 8M34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3343149..3354261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q1I82_RS14465 - 3343696..3344256 (-) 561 WP_037420751.1 DUF4136 domain-containing protein -
  Q1I82_RS14470 - 3344435..3344749 (+) 315 WP_330130877.1 hypothetical protein -
  Q1I82_RS14475 yciH 3344912..3345241 (+) 330 WP_037420757.1 stress response translation initiation inhibitor YciH -
  Q1I82_RS14480 - 3345310..3345873 (+) 564 WP_069453279.1 YqgE/AlgH family protein -
  Q1I82_RS14485 ruvX 3345903..3346328 (+) 426 WP_037420762.1 Holliday junction resolvase RuvX -
  Q1I82_RS14490 hemH 3346392..3347375 (-) 984 WP_240293187.1 ferrochelatase -
  Q1I82_RS14495 - 3347444..3347977 (-) 534 WP_249553409.1 glutathione peroxidase -
  Q1I82_RS14500 pilU 3348149..3349261 (-) 1113 WP_188870156.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q1I82_RS14505 pilT 3349271..3350308 (-) 1038 WP_037420775.1 type IV pilus twitching motility protein PilT Machinery gene
  Q1I82_RS14510 - 3350344..3351057 (+) 714 WP_144375261.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q1I82_RS14515 proC 3351281..3352099 (+) 819 WP_144375262.1 pyrroline-5-carboxylate reductase -
  Q1I82_RS14520 - 3352174..3352722 (+) 549 WP_037420784.1 YggT family protein -
  Q1I82_RS14525 yggU 3352722..3353012 (+) 291 WP_144375264.1 DUF167 family protein YggU -
  Q1I82_RS14530 - 3353086..3353520 (+) 435 WP_037420790.1 DUF4426 domain-containing protein -
  Q1I82_RS14535 rdgB 3353635..3354252 (+) 618 WP_144375268.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41117.33 Da        Isoelectric Point: 6.3725

>NTDB_id=930087 Q1I82_RS14500 WP_188870156.1 3348149..3349261(-) (pilU) [Shewanella xiamenensis strain 8M34]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESVMTEAQKKEFHTTRECNFAFAVKELGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVAKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLQLYVDGEISYADALHHADSPNDLRLMIKLQNKEPASSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=930087 Q1I82_RS14500 WP_188870156.1 3348149..3349261(-) (pilU) [Shewanella xiamenensis strain 8M34]
ATGGATGTCCGTCCGTTTTTAAAAGTCATGGTGGAGCGTAAAGCCTCGGACCTGTTTATTACCGCAGGTTTTCCGCCGAG
CGCCAAAATTGATGGCGAGTTACGCCCATTAGCCGAGAGTGCCTTCACGCCCGCGCAGTCTTTGGATTTTGTCGAGTCCG
TGATGACGGAGGCGCAAAAGAAGGAGTTTCACACCACTCGCGAGTGTAACTTTGCTTTTGCGGTTAAGGAGTTGGGGCGT
TTTCGCGTCAGCGCATTCTGGCAGCGTGAGTCTCCGGGTTGTGTTATGCGCCGGATTGAGACCAAAATCCCTGAAGTGGA
TGATTTAAAACTGCCACCCATTTTAAAAGATCTGGTGATGAGTAAGCGTGGACTTATCATCATGGTGGGGGGAACCGGGA
CCGGTAAATCGACTTCCTTAGCAGCATTAGTCGGTTACCGCAATGCCCATGCCCGCGGGCATATCTTGACGATTGAAGAC
CCTGTGGAATTTGTTCACGATCATCGCAAAAGTATCATTACCCAACGTGAAGTGGGCATAGATACAGAGTCCTTTGATGC
GGCGCTGAAAAGCTCGCTGCGTCAGGCGCCTGATGTGATCCTTATCGGTGAAATTCGAACCCAAGAAACCATGGAGTTTG
CGCTCTCCTTTGCTGAAACGGGCCATCTGTGTATGGCGACACTGCACGCGAACAACGCTAACCAAGCATTAGACCGGATT
ATGCACTTAGTGCCAGAGAGTAAACATCAACAGCTATTATTCGATTTGTCACTCAACCTGCGTGGCATTGTGGCGCAGCA
GCTGGTGGCTAAAGTTGATGGCACAGGGCGCCGGGCGGCGATTGAAGTCTTGATCAATACGCCACGTGTCGCGAGTTTGA
TTGCTAAAAACGAGCTGCATTTGCTCAAAGAAACCATGGCCAAATCGAATGAGCAGGGCATGCAGACCTTTGACCAAGCA
CTGTTACAGCTCTATGTCGATGGGGAGATCAGTTATGCCGACGCGCTTCACCATGCTGACTCACCCAACGACTTGCGGTT
AATGATTAAATTGCAAAATAAAGAACCCGCCAGTTCGAGCTTTATGGAAGGCGTGACCTTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.108

100

0.603
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.293

88.649

0.384
  pilT Vibrio cholerae strain A1552

43.293

88.649

0.384
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.29

93.243

0.376
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Legionella pneumophila strain Lp02

40.785

89.459

0.365
  pilT Legionella pneumophila strain ERS1305867

40.785

89.459

0.365
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362