Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MWM13_RS00090 Genome accession   NZ_AP025609
Coordinates   17852..18937 (-) Length   361 a.a.
NCBI ID   WP_244344598.1    Uniprot ID   -
Organism   Thermus thermophilus strain SNM3-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 12852..23937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM13_RS00075 (TthSNM33_00120) - 14796..15428 (-) 633 WP_244347703.1 histidine phosphatase family protein -
  MWM13_RS00080 (TthSNM33_00130) purM 15425..16426 (-) 1002 WP_143584083.1 phosphoribosylformylglycinamidine cyclo-ligase -
  MWM13_RS00085 (TthSNM33_00140) gatB 16432..17841 (-) 1410 WP_244347704.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  MWM13_RS00090 (TthSNM33_00150) pilT 17852..18937 (-) 1086 WP_244344598.1 type IV pilus twitching motility protein PilT Machinery gene
  MWM13_RS00095 (TthSNM33_00160) pilF 18950..21616 (-) 2667 WP_244344599.1 type IV pilus assembly ATPase PilB Machinery gene
  MWM13_RS00100 (TthSNM33_00170) - 21613..22098 (-) 486 WP_014628818.1 YqeG family HAD IIIA-type phosphatase -
  MWM13_RS00105 (TthSNM33_00180) pgeF 22133..22861 (-) 729 WP_244346785.1 peptidoglycan editing factor PgeF -
  MWM13_RS00110 (TthSNM33_00190) surE 22858..23613 (-) 756 WP_244344600.1 5'/3'-nucleotidase SurE -
  MWM13_RS00115 (TthSNM33_00200) - 23672..23878 (-) 207 WP_011173995.1 cold-shock protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39962.29 Da        Isoelectric Point: 7.5114

>NTDB_id=92999 MWM13_RS00090 WP_244344598.1 17852..18937(-) (pilT) [Thermus thermophilus strain SNM3-3]
MAKAPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPSTVKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLAAMIDYINERKACHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQYGMIT
MDACLADLYRRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=92999 MWM13_RS00090 WP_244344598.1 17852..18937(-) (pilT) [Thermus thermophilus strain SNM3-3]
ATGGCCAAAGCCCCAGACGTGGTGGACCTTTTGGCGCTCGCGGTGGAGCGGGGGGCGAGCGACCTGGTGATCACCGTGGG
CCTGCCCCCCATGCTGAAGATCGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAGCGGATCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGGAAGGGGCGGTACCGGGTCAACGTCTTCCTGCAAAGGGGAAGCGTGGGGGGGGTTCTAAGGGTGGTGCCGAGCACCGT
GAAGAGCTTTGAGGAGCTCGGCCTCCCCAAGAACATCGCCGAGATCGCCATGAGCCCAAGGGGCCTCGTCCTGGTCACGG
GGCCCACAGGGTCCGGCAAGAGCACCACCCTGGCCGCCATGATTGACTACATCAACGAACGCAAGGCCTGCCACATCGTC
ACCATTGAGGACCCCATAGAGTTCTTCCACAAGCACAAGCGGGCCATCGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAGCTTCCACAAGGCGCTCCGCAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTCGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACGGGGCACCTGGTCATGGGCACCCTCCACACCAACTCCGCCCCGGAGACC
ATAGACCGCATCGTGGACGTCTTCCCGGAAAACCAGCAGGAACAGGTCCGGGTGCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACGCAACAGCTCCTGCCCAAGGCCTTTGGCGGCGGGCGGGTCCTGGCCTACGAGCTCATGATCGCCACCCCAGCCG
TGAGGGCCCTCATCCGGGAGGGGAAGACCCACCAGTTAAGGAGCGTGATCCAGACGGGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTCGCCGACCTCTACCGGCGCAAGCTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

68.272

97.784

0.668
  pilT Pseudomonas aeruginosa PAK

54.36

95.291

0.518
  pilT Pseudomonas stutzeri DSM 10701

54.07

95.291

0.515
  pilT Acinetobacter baylyi ADP1

54.07

95.291

0.515
  pilT Acinetobacter baumannii strain A118

53.043

95.568

0.507
  pilT Acinetobacter nosocomialis M2

53.043

95.568

0.507
  pilT Acinetobacter baumannii D1279779

53.043

95.568

0.507
  pilT Neisseria meningitidis 8013

53.254

93.629

0.499
  pilT Neisseria gonorrhoeae MS11

52.522

93.352

0.49
  pilT Legionella pneumophila strain ERS1305867

50.872

95.291

0.485
  pilT Legionella pneumophila strain Lp02

50.872

95.291

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.976

91.136

0.474
  pilT Vibrio cholerae strain A1552

51.976

91.136

0.474
  pilU Pseudomonas stutzeri DSM 10701

46.939

95.014

0.446
  pilU Acinetobacter baylyi ADP1

42.609

95.568

0.407
  pilU Vibrio cholerae strain A1552

45.806

85.873

0.393

Multiple sequence alignment