Detailed information NTG0093000

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: MWM13_RS00095
• Genome accession: NZ_AP025609
• Coordinates: 18950..21616 (-)
• Length: 888 a.a.
• NCBI ID: WP_244344599.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain SNM3-3
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 13950..26616

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MWM13_RS00075 (TthSNM33_00120)	-	14796..15428 (-)	633	WP_244347703.1	histidine phosphatase family protein	-
MWM13_RS00080 (TthSNM33_00130)	purM	15425..16426 (-)	1002	WP_143584083.1	phosphoribosylformylglycinamidine cyclo-ligase	-
MWM13_RS00085 (TthSNM33_00140)	gatB	16432..17841 (-)	1410	WP_244347704.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
MWM13_RS00090 (TthSNM33_00150)	pilT	17852..18937 (-)	1086	WP_244344598.1	type IV pilus twitching motility protein PilT	Machinery gene
MWM13_RS00095 (TthSNM33_00160)	pilF	18950..21616 (-)	2667	WP_244344599.1	type IV pilus assembly ATPase PilB	Machinery gene
MWM13_RS00100 (TthSNM33_00170)	-	21613..22098 (-)	486	WP_014628818.1	YqeG family HAD IIIA-type phosphatase	-
MWM13_RS00105 (TthSNM33_00180)	pgeF	22133..22861 (-)	729	WP_244346785.1	peptidoglycan editing factor PgeF	-
MWM13_RS00110 (TthSNM33_00190)	surE	22858..23613 (-)	756	WP_244344600.1	5'/3'-nucleotidase SurE	-
MWM13_RS00115 (TthSNM33_00200)	-	23672..23878 (-)	207	WP_011173995.1	cold-shock protein	-
MWM13_RS00120 (TthSNM33_00220)	-	24302..25018 (+)	717	WP_244347008.1	transposase	-
MWM13_RS00125 (TthSNM33_00230)	-	25026..25319 (+)	294	Protein_21	ABC transporter substrate-binding protein	-
MWM13_RS00130 (TthSNM33_00240)	-	25371..26246 (+)	876	WP_244341037.1	sugar ABC transporter permease	-

Sequence

Protein

• Length: 888 a.a.
• Molecular weight: 98074.14 Da
• Isoelectric Point: 5.3348

>NTDB_id=93000 MWM13_RS00095 WP_244344599.1 18950..21616(-) (pilF) [Thermus thermophilus strain SNM3-3]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSAFLYALAKHYPELGLPVPPPPSG
EGQKDLKLGELLLQKGWISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESAEGIVPDPSAALLL
RSDALRYGAVPIGFQNGEVEVVLSDPRHKEAVAQLLNRPARFYLALPQAWEELFRRAYPQKNRLGEVLVQEGKLSREALK
EALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRRGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYIDPEEDPPD
PGASLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNYEVAPAVATEAAITKLIERFYGKAELS
EIAKEFAKKQAEEEVPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVIS
VVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKPY
GIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRD
SETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGLS
EAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTLE
EVLARTIE

Nucleotide

• Length: 2667 bp

>NTDB_id=93000 MWM13_RS00095 WP_244344599.1 18950..21616(-) (pilF) [Thermus thermophilus strain SNM3-3]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCTAAGGTCAAG
GCCCTCCTTCCCGCGGAGAAGGCCAAGGAACTCAAGGCCATTCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTCGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAGA
CCACCAAAAGCGCCTTCCTCTACGCTCTGGCCAAGCACTACCCGGAGCTCGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAGAAGGACCTCAAGCTCGGGGAACTTCTCCTGCAAAAGGGGTGGATCTCCCGCGAGGCCCTGGAGGAGGCCTT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CCTTGGCGGAGCAGAAGGGGCTGGAGTTTCTGGAAAGCGCCGAGGGGATCGTCCCAGACCCCTCCGCCGCCCTCCTCCTC
CGCTCCGACGCCCTGCGCTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGGAGGTGGAGGTGGTCCTCTCCGACCCCCG
GCACAAGGAGGCGGTGGCCCAGCTCCTCAATCGGCCTGCCCGCTTCTACCTCGCCCTCCCCCAGGCCTGGGAGGAGCTCT
TCCGCCGGGCCTACCCGCAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAAGCGCTAAAG
GAAGCCCTGGAGGTGCAGAAAGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCGCCC
CGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCGGGGCGGGGGCCGGCTGGAGGACACCCTGGTCCAGTCGGGGAAGC
TCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTATCCCTATATTGACCCCGAGGAGGACCCCCCCGAT
CCCGGCGCCTCCCTCCTCCTCCCGGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAAACCGCCT
CGTCCTCCTGATGAAGGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGAGGAAGGGCCTGAACT
ACGAAGTGGCCCCCGCCGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTCTCG
GAGATCGCCAAGGAGTTCGCCAAAAAGCAGGCGGAGGAGGAGGTCCCAAGCCCCCTGGAGCTGGACGAGAGCGCCGCCCA
GAAGTTCGTGAAGCAGGTGATCCGGGAGGCCTTCCTCCAGGACGCCTCCGACATCCACATTGAGCCCAGGCAGAACGACG
TCCAGGTGCGCCTCCGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCCAAGGGGGCGCTGAACGCGGTGATCTCC
GTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAGCGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGGGGC
CATAGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGGCCT
CGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCCTAC
GGCATCTTCCTCATCACCGGGCCCACGGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTGAAGCGCATCGCCACCCC
CGACAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCCCAGG
CGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGGGAC
TCCGAGACGGCCAAGATCGCCACCGAAGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGCCGC
CCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCCCTTCAACATCTCCGCGGCCCTCATCGGCGTCCTCTCCCAGC
GCCTGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTTTCC
GAGGCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCGCTA
CGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCAAGG
AGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTCGAG
GAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-301)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-478)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

• Type II/IV secretion system protein (T2SSE) (512-778)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	99.55	100	0.997
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.229	100	0.564