Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MWM12_RS00090 Genome accession   NZ_AP025603
Coordinates   17847..18932 (-) Length   361 a.a.
NCBI ID   WP_244341024.1    Uniprot ID   -
Organism   Thermus thermophilus strain SNM1-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 12847..23932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM12_RS00075 (TthSNM17_00120) - 14791..15423 (-) 633 WP_008634004.1 histidine phosphatase family protein -
  MWM12_RS00080 (TthSNM17_00130) purM 15420..16421 (-) 1002 WP_143590140.1 phosphoribosylformylglycinamidine cyclo-ligase -
  MWM12_RS00085 (TthSNM17_00140) gatB 16427..17836 (-) 1410 WP_011227883.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  MWM12_RS00090 (TthSNM17_00150) pilT 17847..18932 (-) 1086 WP_244341024.1 type IV pilus twitching motility protein PilT Machinery gene
  MWM12_RS00095 (TthSNM17_00160) pilF 18945..21611 (-) 2667 WP_244341025.1 type IV pilus assembly ATPase PilB Machinery gene
  MWM12_RS00100 (TthSNM17_00170) - 21608..22093 (-) 486 WP_244341027.1 YqeG family HAD IIIA-type phosphatase -
  MWM12_RS00105 (TthSNM17_00180) pgeF 22128..22856 (-) 729 WP_244341029.1 peptidoglycan editing factor PgeF -
  MWM12_RS00110 (TthSNM17_00190) surE 22853..23608 (-) 756 WP_011173994.1 5'/3'-nucleotidase SurE -
  MWM12_RS00115 (TthSNM17_00200) - 23667..23873 (-) 207 WP_011173995.1 cold-shock protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39990.34 Da        Isoelectric Point: 7.5114

>NTDB_id=92971 MWM12_RS00090 WP_244341024.1 17847..18932(-) (pilT) [Thermus thermophilus strain SNM1-7]
MAKAPDVVDLLALVVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPSTVKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLAAMIDYINERKACHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQYGMIT
MDACLADLYRRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=92971 MWM12_RS00090 WP_244341024.1 17847..18932(-) (pilT) [Thermus thermophilus strain SNM1-7]
ATGGCCAAAGCCCCAGACGTGGTGGACCTTTTGGCGCTCGTGGTGGAGCGGGGGGCGAGCGACCTGGTGATCACCGTGGG
CCTGCCCCCCATGCTGAAGATCGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAGCGGATCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGGAAGGGGCGGTACCGGGTCAACGTCTTCCTGCAGCGGGGAAGCGTGGGGGGGGTTCTAAGGGTGGTGCCGAGCACCGT
GAAGAGCTTTGAGGAGCTCGGCCTCCCCAAGAACATCGCCGAGATCGCCATGAGCCCAAGGGGCCTCGTCCTGGTCACGG
GGCCCACAGGGTCCGGCAAGAGCACCACCCTGGCCGCCATGATCGACTACATCAACGAGCGCAAGGCCTGCCACATCGTC
ACCATTGAGGACCCCATAGAGTTCTTCCACAAGCACAAGCGGGCCATCGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAGCTTCCACAAGGCGCTCCGCAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTCGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACGGGGCACCTGGTCATGGGCACCCTCCACACCAACTCCGCCCCGGAAACC
ATAGACCGCATCGTGGACGTCTTCCCGGAAAACCAGCAGGAACAGGTCCGGGTGCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACGCAACAGCTCCTGCCCAAGGCCTTTGGCGGCGGGCGGGTCCTGGCCTACGAGCTCATGATCGCCACCCCAGCCG
TGAGGGCCCTCATCCGGGAGGGGAAGACCCACCAGTTAAGGAGCGTGATCCAGACGGGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTCGCCGACCTCTACCGGCGCAAGCTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(38-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.989

97.784

0.665
  pilT Pseudomonas aeruginosa PAK

54.36

95.291

0.518
  pilT Pseudomonas stutzeri DSM 10701

54.07

95.291

0.515
  pilT Acinetobacter baylyi ADP1

54.07

95.291

0.515
  pilT Acinetobacter baumannii strain A118

53.043

95.568

0.507
  pilT Acinetobacter nosocomialis M2

53.043

95.568

0.507
  pilT Acinetobacter baumannii D1279779

53.043

95.568

0.507
  pilT Neisseria meningitidis 8013

53.254

93.629

0.499
  pilT Neisseria gonorrhoeae MS11

52.522

93.352

0.49
  pilT Legionella pneumophila strain ERS1305867

50.872

95.291

0.485
  pilT Legionella pneumophila strain Lp02

50.872

95.291

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.976

91.136

0.474
  pilT Vibrio cholerae strain A1552

51.976

91.136

0.474
  pilU Pseudomonas stutzeri DSM 10701

46.939

95.014

0.446
  pilU Acinetobacter baylyi ADP1

42.609

95.568

0.407
  pilU Vibrio cholerae strain A1552

45.806

85.873

0.393

Multiple sequence alignment