Detailed information NTG0092972

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: MWM12_RS00095
• Genome accession: NZ_AP025603
• Coordinates: 18945..21611 (-)
• Length: 888 a.a.
• NCBI ID: WP_244341025.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain SNM1-7
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 13945..26611

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MWM12_RS00075 (TthSNM17_00120)	-	14791..15423 (-)	633	WP_008634004.1	histidine phosphatase family protein	-
MWM12_RS00080 (TthSNM17_00130)	purM	15420..16421 (-)	1002	WP_143590140.1	phosphoribosylformylglycinamidine cyclo-ligase	-
MWM12_RS00085 (TthSNM17_00140)	gatB	16427..17836 (-)	1410	WP_011227883.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
MWM12_RS00090 (TthSNM17_00150)	pilT	17847..18932 (-)	1086	WP_244341024.1	type IV pilus twitching motility protein PilT	Machinery gene
MWM12_RS00095 (TthSNM17_00160)	pilF	18945..21611 (-)	2667	WP_244341025.1	type IV pilus assembly ATPase PilB	Machinery gene
MWM12_RS00100 (TthSNM17_00170)	-	21608..22093 (-)	486	WP_244341027.1	YqeG family HAD IIIA-type phosphatase	-
MWM12_RS00105 (TthSNM17_00180)	pgeF	22128..22856 (-)	729	WP_244341029.1	peptidoglycan editing factor PgeF	-
MWM12_RS00110 (TthSNM17_00190)	surE	22853..23608 (-)	756	WP_011173994.1	5'/3'-nucleotidase SurE	-
MWM12_RS00115 (TthSNM17_00200)	-	23667..23873 (-)	207	WP_011173995.1	cold-shock protein	-
MWM12_RS00120 (TthSNM17_00210)	-	23995..24264 (+)	270	WP_244341031.1	transposase	-
MWM12_RS00125 (TthSNM17_00220)	-	24301..25401 (+)	1101	WP_244341033.1	transposase	-
MWM12_RS00130 (TthSNM17_00230)	-	25408..25593 (-)	186	WP_223966770.1	hypothetical protein	-
MWM12_RS00135 (TthSNM17_00240)	-	25535..26209 (+)	675	WP_244452289.1	transposase	-
MWM12_RS00140 (TthSNM17_00250)	-	26217..26510 (+)	294	Protein_24	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 888 a.a.
• Molecular weight: 98085.04 Da
• Isoelectric Point: 5.2771

>NTDB_id=92972 MWM12_RS00095 WP_244341025.1 18945..21611(-) (pilF) [Thermus thermophilus strain SNM1-7]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELQAIPFALDEEAGVVRVAFVNPLDPLALEEVEDLTGLVVEPYQATKSDFLYALAKNYPELGLPLPPLPSG
PSQRELKLGELLVKKGLLSREALEEALVEQERTGDLLGRILVQKGLSEKDLYRTLAEQHGLESLESTEGLNPDPAATALL
LRSDALRYGAVPVGFREGKVEVVLSDPRHKEAVAQLLNRPARFYLALPQAWEELFRRAYPQKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRRGGGRLEDTLVQSGKLRPEALAQAVAAQLGYPYVDPEEDPP
DPGAPLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLAYEVAPAVATEAAITKLIERFYGKEELG
ELAKELSKGYQEEEAQALADLDESAAQRFVKQVIREAYLQDASDIHIEPRPSDVLVRLRIDGALRQYTTLPKGALNPIIS
VVKIMGNLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKPY
GIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRD
SETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGLS
EAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTLE
EVLARTIE

Nucleotide

• Length: 2667 bp

>NTDB_id=92972 MWM12_RS00095 WP_244341025.1 18945..21611(-) (pilF) [Thermus thermophilus strain SNM1-7]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTGTTGGACGCTGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTGAAG
GCCCTGCTCCCTGCCGAGAAGGCCAAAGAGCTCCAAGCCATCCCCTTCGCCCTGGACGAGGAGGCCGGGGTGGTGCGGGT
GGCCTTCGTCAACCCCTTGGACCCTTTGGCCTTGGAGGAGGTGGAGGACCTCACCGGCTTGGTGGTGGAGCCCTACCAGG
CCACCAAGAGCGACTTCCTCTACGCCCTGGCCAAGAACTACCCCGAGCTCGGTCTGCCCCTTCCCCCTTTGCCCTCGGGC
CCGAGCCAAAGGGAGCTGAAGCTGGGGGAACTCCTGGTGAAGAAGGGCCTCCTTTCCCGAGAGGCCTTGGAGGAGGCCTT
GGTGGAGCAGGAGAGGACGGGGGACCTTCTGGGGCGGATTCTGGTGCAAAAAGGCCTTTCCGAGAAGGACCTCTACCGGA
CCCTGGCCGAGCAGCACGGGCTGGAGTCCCTGGAAAGCACCGAGGGTTTAAATCCTGACCCCGCCGCCACCGCCCTCCTC
CTTCGGTCGGACGCCCTGCGTTACGGGGCCGTGCCCGTGGGCTTTAGGGAGGGGAAGGTGGAGGTGGTCCTCTCCGACCC
CCGGCACAAGGAGGCGGTGGCCCAGCTCCTCAATCGGCCTGCCCGCTTCTACCTCGCCCTCCCCCAGGCCTGGGAGGAGC
TCTTCCGCCGGGCCTACCCGCAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAAGCGCTA
AAGGAAGCCCTGGAGGTGCAGAAAGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCGGGGCGGGGGCCGGCTGGAGGACACCCTGGTCCAGTCGGGGA
AGCTCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCGCCCAGCTGGGGTACCCCTACGTTGACCCCGAGGAGGACCCCCCC
GATCCCGGCGCCCCCCTCCTCCCAGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGGAACCGCCT
CGTCCTCCTGATGAAGGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTGGCCCTGAAGCGGAAGGGCCTCGCCT
ACGAAGTGGCCCCGGCGGTGGCCACGGAGGCGGCCATCACCAAGCTCATTGAGCGCTTCTACGGGAAGGAGGAGCTTGGG
GAGCTCGCCAAGGAGCTCTCCAAGGGCTACCAGGAGGAGGAGGCCCAGGCCCTCGCAGACCTGGACGAAAGCGCCGCCCA
GCGCTTCGTCAAGCAGGTGATCCGGGAGGCCTACCTCCAAGACGCCTCCGACATCCACATTGAGCCCAGGCCTTCGGACG
TCCTGGTGCGCCTCCGGATTGACGGGGCCTTGCGCCAGTACACCACCTTGCCCAAGGGGGCCCTGAACCCCATCATCAGC
GTCGTCAAGATCATGGGGAACCTCAACATCGCCGAGAAGCGCCTCCCCCAAGACGGCCGCGTGCGCTACCGGGAGGGCGC
CATTGACGTGGACCTCCGCCTTTCCACCCTGCCCACGGTGTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGGCCT
CGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCCTAC
GGCATCTTCCTCATCACCGGGCCCACGGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCACCCC
CGACAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCCCAGG
CGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGGGAC
TCCGAGACGGCCAAGATCGCCACCGAAGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGCCGC
CCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCCCTTCAACATCTCCGCGGCCCTCATCGGCGTCCTCTCCCAGC
GCCTGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTTTCC
GAGGCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCGCTA
CGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCAAGG
AGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTCGAG
GAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (512-778)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-478)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	93.138	100	0.932
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.839	100	0.571