Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MWM06_RS00085 Genome accession   NZ_AP025596
Coordinates   16838..17923 (-) Length   361 a.a.
NCBI ID   WP_244360871.1    Uniprot ID   -
Organism   Thermus thermophilus strain SNM1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 11838..22923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM06_RS00070 (TthSNM11_00100) - 13782..14414 (-) 633 WP_244360866.1 histidine phosphatase family protein -
  MWM06_RS00075 (TthSNM11_00110) purM 14411..15412 (-) 1002 WP_244360868.1 phosphoribosylformylglycinamidine cyclo-ligase -
  MWM06_RS00080 (TthSNM11_00120) gatB 15418..16827 (-) 1410 WP_244347704.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  MWM06_RS00085 (TthSNM11_00130) pilT 16838..17923 (-) 1086 WP_244360871.1 type IV pilus twitching motility protein PilT Machinery gene
  MWM06_RS00090 (TthSNM11_00140) pilF 17936..20605 (-) 2670 WP_244360874.1 type IV pilus assembly ATPase PilB Machinery gene
  MWM06_RS00095 (TthSNM11_00150) - 20602..21087 (-) 486 WP_244341027.1 YqeG family HAD IIIA-type phosphatase -
  MWM06_RS00100 (TthSNM11_00160) pgeF 21122..21850 (-) 729 WP_244341029.1 peptidoglycan editing factor PgeF -
  MWM06_RS00105 (TthSNM11_00170) surE 21847..22602 (-) 756 WP_011173994.1 5'/3'-nucleotidase SurE -
  MWM06_RS00110 (TthSNM11_00180) - 22661..22819 (-) 159 WP_244454965.1 cold shock domain-containing protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39944.27 Da        Isoelectric Point: 7.5114

>NTDB_id=92947 MWM06_RS00085 WP_244360871.1 16838..17923(-) (pilT) [Thermus thermophilus strain SNM1-1]
MAKAPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPSTVKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLASMIDYINERKACHIV
TIEDPIEFLHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQYGMIT
MDACLADLYRRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=92947 MWM06_RS00085 WP_244360871.1 16838..17923(-) (pilT) [Thermus thermophilus strain SNM1-1]
ATGGCCAAAGCCCCAGACGTGGTGGACCTTTTGGCGCTCGCGGTGGAGCGGGGGGCGAGCGACCTGGTGATCACCGTGGG
CCTGCCCCCCATGCTGAAGATCGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAGCGGATCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGGAAGGGGCGGTACCGGGTCAACGTCTTCCTGCAAAGGGGAAGCGTGGGGGGGGTTCTAAGGGTGGTGCCGAGCACCGT
GAAGAGCTTTGAGGAGCTCGGCCTCCCCAAGAACATCGCCGAGATCGCCATGAGCCCAAGGGGCCTCGTCCTGGTCACGG
GGCCCACAGGGTCCGGCAAGAGCACCACCCTGGCCTCCATGATTGACTACATCAACGAACGCAAGGCCTGCCACATCGTC
ACCATTGAGGACCCCATAGAGTTCCTCCACAAGCACAAGCGGGCCATCGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAGCTTCCACAAGGCGCTCCGCAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTCGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACAGGGCACCTGGTCATGGGCACCCTCCACACCAACTCCGCCCCGGAAACC
ATAGACCGCATCGTGGACGTCTTCCCGGAAAACCAGCAGGAACAGGTCCGGGTGCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACGCAACAGCTCCTGCCCAAGGCCTTTGGCGGCGGGCGGGTCCTGGCCTACGAGCTCATGATCGCTACCCCAGCCG
TGAGGGCCCTCATCCGGGAGGGGAAGACCCACCAGTTAAGGAGCGTGATCCAGACGGGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTCGCCGACCTCTACCGGCGCAAGCTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

68.555

97.784

0.67
  pilT Pseudomonas aeruginosa PAK

54.07

95.291

0.515
  pilT Pseudomonas stutzeri DSM 10701

53.779

95.291

0.512
  pilT Acinetobacter baylyi ADP1

53.779

95.291

0.512
  pilT Acinetobacter baumannii strain A118

52.754

95.568

0.504
  pilT Acinetobacter nosocomialis M2

52.754

95.568

0.504
  pilT Acinetobacter baumannii D1279779

52.754

95.568

0.504
  pilT Neisseria meningitidis 8013

52.959

93.629

0.496
  pilT Neisseria gonorrhoeae MS11

52.226

93.352

0.488
  pilT Legionella pneumophila strain ERS1305867

50.581

95.291

0.482
  pilT Legionella pneumophila strain Lp02

50.581

95.291

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.672

91.136

0.471
  pilT Vibrio cholerae strain A1552

51.672

91.136

0.471
  pilU Pseudomonas stutzeri DSM 10701

46.647

95.014

0.443
  pilU Acinetobacter baylyi ADP1

42.899

95.568

0.41
  pilU Vibrio cholerae strain A1552

45.484

85.873

0.391

Multiple sequence alignment