Detailed information NTG0092948

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: MWM06_RS00090
• Genome accession: NZ_AP025596
• Coordinates: 17936..20605 (-)
• Length: 889 a.a.
• NCBI ID: WP_244360874.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain SNM1-1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 12936..25605

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MWM06_RS00070 (TthSNM11_00100)	-	13782..14414 (-)	633	WP_244360866.1	histidine phosphatase family protein	-
MWM06_RS00075 (TthSNM11_00110)	purM	14411..15412 (-)	1002	WP_244360868.1	phosphoribosylformylglycinamidine cyclo-ligase	-
MWM06_RS00080 (TthSNM11_00120)	gatB	15418..16827 (-)	1410	WP_244347704.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
MWM06_RS00085 (TthSNM11_00130)	pilT	16838..17923 (-)	1086	WP_244360871.1	type IV pilus twitching motility protein PilT	Machinery gene
MWM06_RS00090 (TthSNM11_00140)	pilF	17936..20605 (-)	2670	WP_244360874.1	type IV pilus assembly ATPase PilB	Machinery gene
MWM06_RS00095 (TthSNM11_00150)	-	20602..21087 (-)	486	WP_244341027.1	YqeG family HAD IIIA-type phosphatase	-
MWM06_RS00100 (TthSNM11_00160)	pgeF	21122..21850 (-)	729	WP_244341029.1	peptidoglycan editing factor PgeF	-
MWM06_RS00105 (TthSNM11_00170)	surE	21847..22602 (-)	756	WP_011173994.1	5'/3'-nucleotidase SurE	-
MWM06_RS00110 (TthSNM11_00180)	-	22661..22819 (-)	159	WP_244454965.1	cold shock domain-containing protein	-
MWM06_RS00115 (TthSNM11_00190)	-	22836..24081 (+)	1246	Protein_19	IS256 family transposase	-
MWM06_RS00120 (TthSNM11_00210)	-	24224..25322 (+)	1099	Protein_20	transposase	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 98133.21 Da
• Isoelectric Point: 5.3969

>NTDB_id=92948 MWM06_RS00090 WP_244360874.1 17936..20605(-) (pilF) [Thermus thermophilus strain SNM1-1]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELQAIPFALDEEAGVVRVAFVNPLDPLALEEVEDLTGLVVEPYQATKSDFLYALAKNYPELGLPLPPLPSG
PSQRELKLGELLVKKGLLSREALEEALVEQERTGDLLGRILVQKGLSEKDLYRTLAEQHGLESLESTEGLNPDPAATALL
LRSDALRYGAVPVGFREGKVEVVLSDPRHKEAVEGLLGRPARFYLTLPQAWEALFQRAYPEKGRLGEVLVQEGRLSREHL
REALEVQKRLPKAKPLGEILVELGLVKPEDVEEALKRQRQGGGRLEDTLVQSGKLKPEALAQAVAAQLGYPYVDPEEDPP
DPGAPLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNYEVAPAVATEAAITKLIERFYGKAEL
SEIAKEFAKKQAEEEVPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVI
SVVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGL
SEAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTL
EEVLARTIE

Nucleotide

• Length: 2670 bp

>NTDB_id=92948 MWM06_RS00090 WP_244360874.1 17936..20605(-) (pilF) [Thermus thermophilus strain SNM1-1]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTGTTGGACGCTGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTGAAG
GCCCTGCTCCCTGCCGAGAAGGCCAAAGAGCTCCAAGCCATCCCCTTCGCCCTGGACGAGGAGGCCGGGGTGGTGCGGGT
GGCCTTCGTCAACCCCTTGGACCCTTTGGCCTTGGAGGAGGTGGAGGACCTCACCGGCTTGGTGGTGGAGCCCTACCAGG
CCACCAAGAGCGACTTCCTCTACGCCCTGGCCAAGAACTACCCCGAGCTCGGTCTGCCCCTTCCCCCTTTGCCCTCGGGC
CCGAGCCAAAGGGAGCTGAAGCTGGGGGAACTCCTGGTGAAGAAGGGCCTCCTTTCCCGAGAGGCCTTGGAGGAGGCCTT
GGTGGAGCAGGAGAGGACGGGGGACCTTCTGGGGCGGATTCTGGTGCAAAAAGGCCTTTCCGAGAAGGACCTCTACCGGA
CCCTGGCCGAGCAGCACGGGCTGGAGTCCCTGGAAAGCACCGAGGGTTTAAATCCTGACCCCGCCGCCACCGCCCTCCTC
CTTCGGTCGGACGCCCTGCGTTACGGGGCCGTGCCCGTGGGCTTTAGGGAGGGGAAGGTGGAGGTGGTCCTCTCTGACCC
CCGGCACAAGGAGGCGGTGGAGGGGCTCTTGGGCCGCCCCGCCCGCTTCTACCTCACCCTACCCCAGGCCTGGGAGGCCC
TTTTCCAGCGGGCCTACCCCGAGAAGGGGCGCCTGGGGGAGGTCTTGGTGCAGGAGGGGCGCCTTTCCCGGGAACACCTC
AGGGAGGCCTTGGAGGTGCAAAAGCGCCTGCCCAAGGCCAAGCCCCTGGGGGAGATCCTGGTGGAGCTTGGCCTCGTCAA
GCCCGAGGACGTGGAGGAGGCCCTGAAGAGGCAGCGCCAGGGCGGGGGCCGTCTGGAGGACACCTTGGTCCAGTCGGGGA
AGCTCAAGCCCGAGGCCCTGGCCCAGGCGGTGGCCGCCCAGCTGGGGTACCCCTACGTTGACCCCGAGGAGGACCCCCCC
GATCCCGGCGCCCCCCTCCTCCTCCCAGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAAACCG
CCTCGTCCTCCTGATGAAGGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGAGGAAGGGCCTGA
ACTACGAAGTGGCCCCCGCCGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAGTTCGCCAAAAAGCAGGCGGAGGAGGAGGTCCCAAGCCCCCTGGAGCTGGACGAGAGCGCCGC
CCAGAAGTTCGTGAAGCAGGTGATCCGGGAGGCCTTCCTCCAGGACGCCTCCGACATCCACATTGAGCCCAGGCAGAACG
ACGTCCAGGTGCGCCTCCGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCCAAGGGGGCGCTGAACGCGGTGATC
TCCGTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAGCGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGG
GGCCATAGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGG
CCTCGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGGCCCACGGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCCC
AGGCGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGG
GACTCCGAGACGGCCAAGATCGCCACCGAAGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCCCTTCAACATCTCCGCGGCCCTCATCGGCGTCCTCTCCC
AGCGCCTGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTT
TCCGAGGCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCG
CTACGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCA
AGGAGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (513-779)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	93.926	100	0.939
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.726	100	0.569