Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VR487_RS06305 Genome accession   NZ_CP143431
Coordinates   1416392..1417429 (+) Length   345 a.a.
NCBI ID   WP_006082517.1    Uniprot ID   A0AA50Q7P7
Organism   Shewanella oncorhynchi strain B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1411392..1422429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VR487_RS06275 (VR487_06275) rdgB 1412413..1413030 (-) 618 WP_131523655.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  VR487_RS06280 (VR487_06280) - 1413153..1413587 (-) 435 WP_113939419.1 DUF4426 domain-containing protein -
  VR487_RS06285 (VR487_06285) yggU 1413723..1414028 (-) 306 WP_263162135.1 DUF167 family protein YggU -
  VR487_RS06290 (VR487_06290) - 1414028..1414576 (-) 549 WP_105251874.1 YggT family protein -
  VR487_RS06295 (VR487_06295) proC 1414623..1415441 (-) 819 WP_263162138.1 pyrroline-5-carboxylate reductase -
  VR487_RS06300 (VR487_06300) - 1415658..1416356 (-) 699 WP_212600718.1 YggS family pyridoxal phosphate-dependent enzyme -
  VR487_RS06305 (VR487_06305) pilT 1416392..1417429 (+) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  VR487_RS06310 (VR487_06310) pilU 1417439..1418551 (+) 1113 WP_028761282.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VR487_RS06315 (VR487_06315) - 1418738..1419277 (+) 540 WP_330148283.1 glutathione peroxidase -
  VR487_RS06320 (VR487_06320) hemH 1419310..1420323 (+) 1014 WP_263196114.1 ferrochelatase -
  VR487_RS06325 (VR487_06325) ruvX 1420411..1420836 (-) 426 WP_028761279.1 Holliday junction resolvase RuvX -
  VR487_RS06330 (VR487_06330) - 1420866..1421429 (-) 564 WP_330148284.1 YqgE/AlgH family protein -
  VR487_RS06335 (VR487_06335) yciH 1421496..1421825 (-) 330 WP_219253466.1 stress response translation initiation inhibitor YciH -
  VR487_RS06340 (VR487_06340) - 1422004..1422321 (-) 318 WP_263162154.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.94 Da        Isoelectric Point: 6.9095

>NTDB_id=929037 VR487_RS06305 WP_006082517.1 1416392..1417429(+) (pilT) [Shewanella oncorhynchi strain B]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=929037 VR487_RS06305 WP_006082517.1 1416392..1417429(+) (pilT) [Shewanella oncorhynchi strain B]
ATGGAAATCACTGAGTTATTGGCATTTAGTGTAAAACACAAAGCCTCGGATCTACACCTTTCTGCAGGGATATCTCCTAT
GATCCGTGTTGACGGTGAAGTGAGAAAAATTAACCTGCCCGCGCTCGATCATCAAGGTGTACACAGTTTAGTGTACGACA
TAATGAATGATAAGCAGCGTAAGGACTACGAAGAACATTTAGAAATCGATTTCTCGTTCGAAGTCCCTAATCTTGCACGC
TTTCGTGTTAACGCATTCAATCAATCCCGTGGTGCAGCGGCTGTGTTTCGTACCATTCCAAGTGACATTTTGTCACTTGA
GCAATTAGGTGCACCGGAAATTTTTAAAAAAATTGCCAGTTTCCCTCGGGGATTAGTGCTAGTGACTGGACCTACGGGGT
CGGGTAAGAGTACCACACTTGCCGCTATGGTGGATTACGTGAATGAAAATCGACATGATCATATCTTAACCATTGAAGAT
CCTATCGAATTCGTTCACCAAAATAAGCAATGTTTGATTAACCAACGTGAAGTTCACCGCCATACCCACAGCTTTAATGC
GGCGCTTAGAAGCGCACTGCGTGAAGACCCAGACGTGATTTTAGTCGGTGAGATGCGTGACCTTGAGACCATCCGTTTGG
CTATGACCGCCGCTGAAACAGGCCACTTAGTGTTTGGTACCTTGCATACTACTTCTGCCGCGAAAACGATTGACCGTGTG
GTCGACGTATTCCCTGCTGGTGAAAAAGACATGGTGCGCACTATGTTGTCTGAATCGCTGCAAGCGGTTATTTCGCAAAC
CCTGATTAAAAAGGTGGGAGGAGGCCGTGTCGCCGCCCATGAAATCATGATGGGCACGCCCGCTATCCGCAACTTAATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGGCAATTCAAACAGGTATGGCCCATGGAATGCAGACCTTAGAGCAATGTTTA
CAGAACTTAGTCAATCGCGGTCTTATCACTCGTGAAGATGCAATGGCGAAAAGTTCGAATAAACAAGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.681

0.716
  pilT Vibrio cholerae strain A1552

73.294

97.681

0.716
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388