Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VR487_RS06310 Genome accession   NZ_CP143431
Coordinates   1417439..1418551 (+) Length   370 a.a.
NCBI ID   WP_028761282.1    Uniprot ID   -
Organism   Shewanella oncorhynchi strain B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1412439..1423551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VR487_RS06280 (VR487_06280) - 1413153..1413587 (-) 435 WP_113939419.1 DUF4426 domain-containing protein -
  VR487_RS06285 (VR487_06285) yggU 1413723..1414028 (-) 306 WP_263162135.1 DUF167 family protein YggU -
  VR487_RS06290 (VR487_06290) - 1414028..1414576 (-) 549 WP_105251874.1 YggT family protein -
  VR487_RS06295 (VR487_06295) proC 1414623..1415441 (-) 819 WP_263162138.1 pyrroline-5-carboxylate reductase -
  VR487_RS06300 (VR487_06300) - 1415658..1416356 (-) 699 WP_212600718.1 YggS family pyridoxal phosphate-dependent enzyme -
  VR487_RS06305 (VR487_06305) pilT 1416392..1417429 (+) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  VR487_RS06310 (VR487_06310) pilU 1417439..1418551 (+) 1113 WP_028761282.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VR487_RS06315 (VR487_06315) - 1418738..1419277 (+) 540 WP_330148283.1 glutathione peroxidase -
  VR487_RS06320 (VR487_06320) hemH 1419310..1420323 (+) 1014 WP_263196114.1 ferrochelatase -
  VR487_RS06325 (VR487_06325) ruvX 1420411..1420836 (-) 426 WP_028761279.1 Holliday junction resolvase RuvX -
  VR487_RS06330 (VR487_06330) - 1420866..1421429 (-) 564 WP_330148284.1 YqgE/AlgH family protein -
  VR487_RS06335 (VR487_06335) yciH 1421496..1421825 (-) 330 WP_219253466.1 stress response translation initiation inhibitor YciH -
  VR487_RS06340 (VR487_06340) - 1422004..1422321 (-) 318 WP_263162154.1 hypothetical protein -
  VR487_RS06345 (VR487_06345) - 1422502..1423062 (+) 561 WP_028761276.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41029.16 Da        Isoelectric Point: 6.3720

>NTDB_id=929038 VR487_RS06310 WP_028761282.1 1417439..1418551(+) (pilU) [Shewanella oncorhynchi strain B]
MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=929038 VR487_RS06310 WP_028761282.1 1417439..1418551(+) (pilU) [Shewanella oncorhynchi strain B]
ATGGATGTTCGTCCGTTTTTAAAAGTGATAGTGGAACGCAAAGCCTCCGACTTGTTCATCACAGCGGGTTTTCCGCCGAG
TGCCAAGATTGACGGTGAATTGCGTCCATTGGCAGAAAGTGCGTTTACACCAGCACAATCCCTGGATTTTGTTGAGTCGT
TAATGACTGAGGCTCAGAAGCAAGAGTTTCATTCCACTCGTGAGTGTAACTTCGCTTTTGCGGCAAAGGATTTAGGTCGT
TTTCGGGTGAGCGCTTTTTGGCAGCGTGAATCGCCTGGCTGCGTGATGCGCCGTATTGAGACTAAAATCCCTGATGTGGA
TGATTTAAAATTGCCGCCTATTCTAAAAGATCTGGTGATGAGTAAACGTGGTCTCATCATTATGGTAGGCGGAACGGGAA
CAGGTAAGTCAACGTCCTTGGCGGCCTTGGTGGGTTATCGCAATGGTCATGCTCGTGGGCATATTTTGACTATTGAAGAT
CCGGTTGAATTCGTTCATGACCACAGAAAAAGCATAATCACTCAGCGTGAAGTGGGTATAGATACTGAATCTTTCGATGC
GGCGCTAAAAAGCTCCCTTCGCCAAGCTCCCGATGTGATCTTGATTGGTGAAATCCGTACCCAAGAGACGATGGAGTTTG
CGCTGTCATTTGCAGAAACGGGTCACCTTTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCGTTAGACCGGATT
ATGCATCTCGTGCCTGAGAGTAAGCATCAACAGTTATTGTTCGATTTATCATTGAATTTACGCGGTATTGTGGCGCAACA
ATTGATACCGAAAGTCGATGGCACAGGGCGTCGCGCCGCGATTGAAGTATTGATTAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAACGAGCTGCATTTACTCAAAGAAACCATGGCAAAATCCAACGAGCAGGGCATGCAGACGTTCGATCAGGCC
TTGCTGAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCAAACGATTTACGTTT
GATGATTAAATTGCAGAATAAAGAATCCACGGGCTCAGGTTTTATGGAAGGTGTCACCTTAGACATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362