Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KAN5_RS11155 Genome accession   NZ_AP025589
Coordinates   2494501..2495544 (-) Length   347 a.a.
NCBI ID   WP_016708192.1    Uniprot ID   A0A5S3RSP2
Organism   Pseudoalteromonas sp. KAN5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2489501..2500544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAN5_RS11125 (KAN5_21730) - 2489738..2490211 (+) 474 WP_244389711.1 hypothetical protein -
  KAN5_RS11130 (KAN5_21740) rsmE 2490297..2491031 (+) 735 WP_244389712.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KAN5_RS11135 (KAN5_21750) gshB 2491086..2492039 (+) 954 WP_016708187.1 glutathione synthase -
  KAN5_RS11140 (KAN5_21760) - 2492067..2492624 (+) 558 WP_016708188.1 YqgE/AlgH family protein -
  KAN5_RS11145 (KAN5_21770) ruvX 2492661..2493107 (+) 447 WP_016708189.1 Holliday junction resolvase RuvX -
  KAN5_RS11150 (KAN5_21780) pilU 2493371..2494501 (-) 1131 WP_016708191.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KAN5_RS11155 (KAN5_21790) pilT 2494501..2495544 (-) 1044 WP_016708192.1 type IV pilus twitching motility protein PilT Machinery gene
  KAN5_RS11160 (KAN5_21800) - 2495547..2496260 (+) 714 WP_016708193.1 YggS family pyridoxal phosphate-dependent enzyme -
  KAN5_RS11165 (KAN5_21810) proC 2496276..2497097 (+) 822 WP_244389713.1 pyrroline-5-carboxylate reductase -
  KAN5_RS11170 (KAN5_21820) - 2497147..2497680 (+) 534 WP_016708195.1 YggT family protein -
  KAN5_RS11175 (KAN5_21830) - 2497736..2498179 (+) 444 WP_244389714.1 DUF4426 domain-containing protein -
  KAN5_RS11180 (KAN5_21840) - 2498183..2498782 (+) 600 WP_244389715.1 XTP/dITP diphosphatase -
  KAN5_RS11185 (KAN5_21850) hemW 2498779..2499915 (+) 1137 WP_244389716.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38289.54 Da        Isoelectric Point: 6.3876

>NTDB_id=92879 KAN5_RS11155 WP_016708192.1 2494501..2495544(-) (pilT) [Pseudoalteromonas sp. KAN5]
MDITELLAFSVQHKASDLHLSSGVAPMIRVDGDVRRINIPALENKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLDDLGAPDIFKTISDTPRGLVLVTGPTGSGKSTTLAAMVDYINQHKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKSMIRSMLSESLRAVISQTLLKKVGGGRVAAHEIMIAVPAIRNLIREDKIAQMYSSIQTGASHGMQTMDQCL
TNLVNHGVVTNAEARAKAQDKNNFGGQ

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=92879 KAN5_RS11155 WP_016708192.1 2494501..2495544(-) (pilT) [Pseudoalteromonas sp. KAN5]
ATGGATATTACCGAATTATTGGCCTTTAGTGTGCAGCACAAAGCATCCGATTTACACTTATCATCAGGGGTTGCGCCAAT
GATCCGTGTTGATGGCGATGTGCGACGTATTAATATACCAGCGCTGGAAAACAAAGATGTGAACAGCCTAGTTTACGACA
TTATGAACGATAATCAACGCAAGGACTACGAACAAAACTTAGAAGTGGACTTTTCGTTTGAAGTGCCAAATTTAGCACGT
TTTCGTGTTAATGCCTTTAACTCAAACCGTGGCCCTGCAGCGGTATTTCGTACTATCCCTAGCGAAGTTTTAACACTCGA
TGATTTAGGCGCCCCCGATATCTTCAAAACAATCTCAGATACGCCACGCGGCTTGGTACTCGTTACTGGGCCAACCGGTT
CTGGTAAATCAACCACCTTAGCTGCAATGGTCGATTATATTAACCAACATAAACATCATCATATTTTAACTATTGAAGAT
CCAATCGAGTTTGTTCATGACAATAAATTATCACTGATCAACCAGCGTGAAGTTCACCGTGATACTCATAGTTTCTCTGC
AGCACTGCGTAGCGCTTTGCGTGAAGACCCTGATGTTATACTAGTGGGTGAGCTACGTGATTTAGAAACCATTCGCCTTG
CTATGACCGCGGCTGAAACTGGTCATCTAGTATTTGGTACTTTGCATACTACCTCAGCACCAAAAACCATTGACCGTATT
ATTGATGTATTCCCTGGCGAAGAAAAAAGCATGATCCGCTCAATGCTTTCAGAATCATTGCGTGCGGTTATTTCACAAAC
TTTATTGAAAAAAGTGGGCGGGGGACGAGTCGCAGCCCATGAAATTATGATTGCAGTGCCTGCGATCCGTAACTTGATCC
GCGAAGATAAAATTGCGCAAATGTATTCATCTATTCAAACAGGTGCATCACACGGTATGCAAACGATGGATCAATGTTTA
ACCAATTTAGTAAACCACGGTGTAGTTACCAATGCTGAGGCGCGCGCTAAAGCACAAGATAAAAATAACTTTGGTGGACA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S3RSP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.744

99.135

0.761
  pilT Acinetobacter nosocomialis M2

76.744

99.135

0.761
  pilT Acinetobacter baumannii strain A118

76.744

99.135

0.761
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.135

0.758
  pilT Acinetobacter baylyi ADP1

75.872

99.135

0.752
  pilT Pseudomonas aeruginosa PAK

75.581

99.135

0.749
  pilT Legionella pneumophila strain Lp02

72.674

99.135

0.72
  pilT Legionella pneumophila strain ERS1305867

72.674

99.135

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.681

97.695

0.7
  pilT Vibrio cholerae strain A1552

71.681

97.695

0.7
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.424

0.519
  pilU Vibrio cholerae strain A1552

40.87

99.424

0.406
  pilU Acinetobacter baylyi ADP1

39.595

99.712

0.395
  pilU Pseudomonas stutzeri DSM 10701

38.841

99.424

0.386

Multiple sequence alignment