Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KAN5_RS11150 Genome accession   NZ_AP025589
Coordinates   2493371..2494501 (-) Length   376 a.a.
NCBI ID   WP_016708191.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. KAN5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2488371..2499501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAN5_RS11120 (KAN5_21720) - 2488814..2489707 (+) 894 WP_016708184.1 hypothetical protein -
  KAN5_RS11125 (KAN5_21730) - 2489738..2490211 (+) 474 WP_244389711.1 hypothetical protein -
  KAN5_RS11130 (KAN5_21740) rsmE 2490297..2491031 (+) 735 WP_244389712.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KAN5_RS11135 (KAN5_21750) gshB 2491086..2492039 (+) 954 WP_016708187.1 glutathione synthase -
  KAN5_RS11140 (KAN5_21760) - 2492067..2492624 (+) 558 WP_016708188.1 YqgE/AlgH family protein -
  KAN5_RS11145 (KAN5_21770) ruvX 2492661..2493107 (+) 447 WP_016708189.1 Holliday junction resolvase RuvX -
  KAN5_RS11150 (KAN5_21780) pilU 2493371..2494501 (-) 1131 WP_016708191.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KAN5_RS11155 (KAN5_21790) pilT 2494501..2495544 (-) 1044 WP_016708192.1 type IV pilus twitching motility protein PilT Machinery gene
  KAN5_RS11160 (KAN5_21800) - 2495547..2496260 (+) 714 WP_016708193.1 YggS family pyridoxal phosphate-dependent enzyme -
  KAN5_RS11165 (KAN5_21810) proC 2496276..2497097 (+) 822 WP_244389713.1 pyrroline-5-carboxylate reductase -
  KAN5_RS11170 (KAN5_21820) - 2497147..2497680 (+) 534 WP_016708195.1 YggT family protein -
  KAN5_RS11175 (KAN5_21830) - 2497736..2498179 (+) 444 WP_244389714.1 DUF4426 domain-containing protein -
  KAN5_RS11180 (KAN5_21840) - 2498183..2498782 (+) 600 WP_244389715.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41672.76 Da        Isoelectric Point: 6.4359

>NTDB_id=92878 KAN5_RS11150 WP_016708191.1 2493371..2494501(-) (pilU) [Pseudoalteromonas sp. KAN5]
MTHSLDYFLRLMLEKQGSDLFVSSQLPVSAKINGELIALGDEKISDEESLALVESAMNEKQKAEFHSTKECNFAIATDEG
RFRISAFWQRDRAGMVIRRIVTKIPDVTELGLPSTLTDVIMAKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLSLNLRAIIAQQLVPTADGNGRVAAIEILLNTPIVGELIKKGDIGGIKEAMAKAKDMGMQTFDQ
ALFELYKQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLEPKGQ

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=92878 KAN5_RS11150 WP_016708191.1 2493371..2494501(-) (pilU) [Pseudoalteromonas sp. KAN5]
ATGACACATTCTCTAGATTACTTTTTACGATTGATGCTTGAAAAGCAAGGGTCTGATTTATTTGTTTCAAGTCAATTGCC
GGTCAGCGCTAAGATTAATGGTGAGTTGATTGCATTAGGTGACGAAAAAATATCAGATGAGGAGTCGCTCGCGTTAGTCG
AATCAGCGATGAATGAAAAACAAAAAGCAGAGTTTCATAGTACTAAAGAATGTAATTTTGCAATTGCTACTGATGAAGGG
CGTTTTAGGATCTCGGCATTTTGGCAACGTGATCGTGCCGGTATGGTTATTCGCCGCATTGTTACAAAAATTCCAGACGT
CACAGAGCTTGGTCTACCTTCAACGCTGACAGATGTGATCATGGCGAAGCGGGGCCTAGTGTTATTCGTTGGTGGTACTG
GGACGGGTAAATCGACCTCTCTTGCTGCACTATTAGGTTATCGTAACCGTAATCAACGTGGACATATTTTAACTATTGAA
GATCCCATTGAATTTGTACATGAACATCGAAAAAGTATTATTACCCAACGTGAAGTTGGCCTTGATACGGAAAGCTTTGA
ATCGGCTCTTAAAAGTTCATTGCGCCAAGCTCCAGATGTTATTTTAATTGGTGAAATACGCTCACAAGAGACCATGGAAT
ATGCACTGAGTTTTGCTGAAACAGGGCATTTATGTGTGGCAACTCTGCATGCTAATAATGCTAACCAAGCGATTGATCGG
ATCATGCATTTAGTGCCAAAAGAAAAGCACGACAAGTTAAAATATGACCTATCTTTAAATTTACGCGCTATTATTGCGCA
ACAGTTAGTGCCTACCGCTGATGGCAATGGCCGTGTGGCTGCGATCGAAATTTTGCTGAATACGCCAATTGTTGGCGAGC
TTATCAAAAAAGGTGACATTGGCGGTATAAAAGAAGCCATGGCGAAAGCAAAAGACATGGGTATGCAAACTTTTGATCAG
GCATTGTTTGAACTTTATAAGCAGCAGCGAATTAACTATGCTGATGCATTGCACCACGCTGACTCACCAAACGATTTACG
TTTGATGATTAAGCTTAGAAACAATGAACAGCAAGGAGCTGGCTTTTTACAAGGAGTTACCATCGATGGGCTAGAGCCCA
AAGGTCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.172

92.819

0.559
  pilU Acinetobacter baylyi ADP1

55.645

98.936

0.551
  pilU Vibrio cholerae strain A1552

56.712

97.074

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.231

96.809

0.428
  pilT Pseudomonas aeruginosa PAK

40.719

88.83

0.362
  pilT Acinetobacter baylyi ADP1

40.356

89.628

0.362

Multiple sequence alignment