Detailed information    

experimental Experimentally validated

Overview

Name   comM   Type   Machinery gene
Locus tag   C694_RS04030 Genome accession   NC_018939
Coordinates   848974..850494 (-) Length   506 a.a.
NCBI ID   WP_000611139.1    Uniprot ID   O25480
Organism   Helicobacter pylori 26695     
Function   interact with DprA   
Homologous recombination

Function

Using Helicobacter pylori and Vibrio cholerae as model systems, it was demonstrated that ComM directly interacts with the DprA winged-helix domain, and that this interaction is critical for DprA to recruit ComM to the recombination site to promote branch migration during NT.


Genomic Context

Location: 843974..855494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C694_RS04015 (C694_04045) - 845281..845415 (-) 135 Protein_758 restriction endonuclease subunit R -
  C694_RS04020 (C694_04050) - 845552..846847 (-) 1296 WP_000548846.1 restriction endonuclease subunit S -
  C694_RS04025 (C694_04055) - 846889..848949 (-) 2061 WP_001158808.1 heavy metal translocating P-type ATPase -
  C694_RS04030 (C694_04060) comM 848974..850494 (-) 1521 WP_000611139.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  C694_RS04035 (C694_04065) def 850500..851024 (-) 525 WP_001185845.1 peptide deformylase -
  C694_RS04040 (C694_04070) clpP 851029..851616 (-) 588 WP_000540573.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  C694_RS04045 (C694_04075) tig 851637..852992 (-) 1356 WP_001047731.1 trigger factor -
  C694_RS04050 (C694_04080) - 853104..853940 (-) 837 WP_001037790.1 outer membrane protein -
  C694_RS04055 (C694_04085) hpaA 853969..854751 (-) 783 WP_000646667.1 flagellar sheath lipoprotein HpaA -
  C694_RS04060 (C694_04090) moaC 854870..855346 (-) 477 WP_001131513.1 cyclic pyranopterin monophosphate synthase MoaC -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 57127.16 Da        Isoelectric Point: 8.3913

>NTDB_id=1478 C694_RS04030 WP_000611139.1 848974..850494(-) (comM) [Helicobacter pylori 26695]
MINTMFCATMQRGVAEIVAVEATFTRALPAFVISGLANSSIQEAKQRVQSALQNNDFTFPPLKITINLSPSDLPKSGSHF
DLPIALLIALQKQELAFKEWFAFGELGLDGKIKPNPNIFPMLLDIAIKHPHAKIIAPKANEELFSLIPNLQCFFVGHFKE
ALEILQNPETKADTHTKKLPFKTIELNDKEYYFSDAYALDFKEVKGQAVAKEAALIASAGFHNLILEGSPGCGKSMIINR
MRYILPPLSLNEILEATKLRILSEQDSAYYPLRSFRNPHQSASKSSILGSSSLREPKPGEIALAHNGMLFFDELPHFKKD
ILEALREPLENNKLVVSRVHSKIEYETSFLFVGAQNPCLCGNLLSATKACRCQDREITQYKNRLSEPFLDRIDLFVQMEE
GNYKDTPSHSWTSKEMHELVLLAFKQQKLRKQSVFNGKLNEEQIERFCPLNAEAKKLLEQAVERFNLSMRSINKVKKVAR
TIADLNACEDIEKSHMLKALSFRKIS

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=1478 C694_RS04030 WP_000611139.1 848974..850494(-) (comM) [Helicobacter pylori 26695]
ATGATTAACACGATGTTTTGCGCGACCATGCAAAGGGGAGTGGCGGAAATCGTGGCTGTGGAAGCGACTTTCACAAGGGC
TTTGCCGGCGTTTGTGATTTCAGGGTTAGCTAATAGCTCTATCCAAGAAGCCAAACAGCGGGTTCAATCGGCTTTACAAA
ATAACGATTTCACTTTCCCGCCTTTAAAAATCACCATCAACCTTTCCCCCTCAGATTTGCCTAAATCCGGGAGTCATTTT
GATTTGCCTATCGCTCTTTTAATCGCTTTGCAAAAACAAGAGTTGGCTTTTAAAGAGTGGTTTGCTTTTGGGGAGTTAGG
GCTTGATGGCAAGATCAAACCCAATCCTAACATTTTCCCCATGCTTTTAGACATTGCCATTAAACACCCCCATGCTAAGA
TCATTGCGCCTAAGGCCAATGAAGAGCTTTTTTCGCTTATCCCTAATTTGCAATGCTTTTTTGTGGGGCATTTTAAAGAA
GCGTTAGAAATCTTGCAAAACCCTGAAACCAAAGCAGACACCCACACGAAAAAACTACCCTTTAAAACGATAGAATTGAA
CGATAAAGAGTATTATTTTTCAGACGCCTATGCCTTAGATTTTAAAGAAGTTAAGGGGCAAGCTGTCGCTAAAGAGGCCG
CTTTGATCGCTAGCGCTGGGTTTCATAACTTGATTTTAGAGGGAAGTCCAGGGTGTGGGAAAAGCATGATCATTAATCGC
ATGCGTTATATCTTGCCTCCATTAAGCCTGAATGAAATCCTAGAAGCGACAAAATTACGCATTTTAAGCGAGCAAGACAG
TGCCTATTACCCCTTAAGGAGTTTTAGAAACCCTCACCAAAGCGCTTCAAAATCCAGTATTTTAGGCTCAAGCTCTCTAA
GAGAGCCAAAACCTGGCGAAATCGCGCTAGCGCATAACGGCATGCTTTTTTTTGATGAATTGCCTCATTTTAAAAAGGAT
ATTTTGGAAGCTTTAAGAGAGCCTTTAGAAAACAATAAATTGGTGGTTTCAAGAGTGCATAGCAAAATTGAATACGAAAC
CTCTTTTTTATTTGTAGGGGCTCAAAACCCTTGCTTGTGCGGGAATTTACTCAGCGCGACCAAAGCATGCCGTTGCCAAG
ACAGAGAAATCACGCAGTATAAAAACCGCTTGAGCGAGCCTTTTTTGGATAGGATTGATTTGTTTGTGCAAATGGAAGAG
GGGAATTATAAAGACACGCCGTCGCATTCTTGGACTTCAAAAGAGATGCATGAATTGGTGTTATTAGCTTTCAAGCAGCA
AAAGTTAAGGAAACAGAGCGTTTTTAATGGTAAGCTTAATGAAGAGCAGATAGAACGATTTTGCCCCTTAAACGCTGAAG
CAAAAAAGTTGTTGGAGCAGGCGGTTGAAAGGTTTAATCTCTCCATGCGCTCTATTAATAAGGTCAAAAAAGTCGCTAGG
ACGATTGCGGATTTAAACGCTTGCGAGGATATAGAAAAATCTCACATGCTTAAAGCGCTGAGTTTTAGAAAGATTTCTTA
A

Domains

Predicted by InterProScan.

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(200-403)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(412-503)

Subunit ChlI of Mg-chelatase (ChlI)

(20-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O25480

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter baylyi ADP1

36.008

100

0.372
  comM Legionella pneumophila str. Paris

36.183

100

0.362
  comM Legionella pneumophila strain ERS1305867

36.183

100

0.362

References

[1] Triana N Dalia et al. (2025) DprA recruits ComM to facilitate recombination during natural transformation in Gram-negative bacteria. Proceedings of The National Academy of Sciences of The United States of America 122(15):e2421764122. [PMID: 40215278]