Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SA496_RS27360 Genome accession   NZ_CP143312
Coordinates   5823391..5824425 (+) Length   344 a.a.
NCBI ID   WP_028629028.1    Uniprot ID   -
Organism   Pseudomonas sp. JS3066     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5818391..5829425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA496_RS27335 (SA496_27335) - 5818596..5820584 (-) 1989 WP_330114759.1 dynamin-like GTPase family protein -
  SA496_RS27340 (SA496_27340) - 5820795..5821091 (-) 297 WP_120653590.1 DUF167 domain-containing protein -
  SA496_RS27345 (SA496_27345) - 5821099..5821692 (-) 594 WP_120653589.1 YggT family protein -
  SA496_RS27350 (SA496_27350) proC 5821703..5822521 (-) 819 WP_120653588.1 pyrroline-5-carboxylate reductase -
  SA496_RS27355 (SA496_27355) - 5822532..5823227 (-) 696 WP_330114760.1 YggS family pyridoxal phosphate-dependent enzyme -
  SA496_RS27360 (SA496_27360) pilT 5823391..5824425 (+) 1035 WP_028629028.1 type IV pilus twitching motility protein PilT Machinery gene
  SA496_RS27365 (SA496_27365) pilU 5824453..5825598 (+) 1146 WP_330114761.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SA496_RS27370 (SA496_27370) - 5825674..5826069 (+) 396 WP_120653585.1 TM2 domain-containing protein -
  SA496_RS27375 (SA496_27375) - 5826215..5827594 (+) 1380 WP_330114762.1 pyridoxal-phosphate dependent enzyme -
  SA496_RS27380 (SA496_27380) - 5827591..5828775 (+) 1185 WP_330114763.1 PLP-dependent aspartate aminotransferase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37901.68 Da        Isoelectric Point: 6.8361

>NTDB_id=928657 SA496_RS27360 WP_028629028.1 5823391..5824425(+) (pilT) [Pseudomonas sp. JS3066]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEIFKKISDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLVAKGLVSRESAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=928657 SA496_RS27360 WP_028629028.1 5823391..5824425(+) (pilT) [Pseudomonas sp. JS3066]
ATGGACATTACTGAGCTGCTTGCCTTCAGCGCCAAGCAGGGCGCTTCGGACCTGCATCTCTCGGCCGGCCTGCCACCCAT
GATTCGCGTCGATGGCGACGTGCGCCGGATCAACCTTCCGGCCCTGGACCACAAGCAGGTCCACGCGTTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAGTTCCTCGAGACAGACTTCTCCTTCGAAGTCCCGGGCGTCGCACGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCTTCCAAGGTGTTGAGCATGGA
GGACCTGGGGATGGGAGAGATCTTCAAGAAGATCTCAGACGTTCCCCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGCTCGACTACCTGAACAGCACCAAGTACCACCACATCCTCACCGTGGAAGAT
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGATCAACCAGCGGGAAGTGCACCGCGATACGCTCGGCTTCAACGA
AGCCCTGCGCTCGGCACTGCGTGAAGACCCGGACATCATCCTGGTGGGTGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGCGTG
GTCGACGTATTCCCTGCCGAGGAAAAGTCCATGGTCCGCTCCATGCTCTCCGAGTCCCTGCAGTCGGTGATTTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGGGTGGCGGCTCACGAGATCATGATCGGCACCCCGGCAATCCGTAACCTGATTC
GCGAAGACAAGGTGGCGCAGATGTATTCCGCCATCCAGACCGGCGGCTCGCTCGGCATGCAGACCCTCGACATGTGCCTC
AAGGGGCTTGTCGCCAAGGGACTGGTCAGTCGTGAAAGCGCGCGGGAAAAGGCCAAGAGCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.349

100

0.953
  pilT Pseudomonas stutzeri DSM 10701

94.477

100

0.945
  pilT Acinetobacter baumannii D1279779

85.756

100

0.858
  pilT Acinetobacter baumannii strain A118

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

85.465

100

0.855
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392