Detailed information NTG0928658

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SA496_RS27365
• Genome accession: NZ_CP143312
• Coordinates: 5824453..5825598 (+)
• Length: 381 a.a.
• NCBI ID: WP_330114761.1
• Uniprot ID: -
• Organism: Pseudomonas sp. JS3066
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5819453..5830598

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SA496_RS27340 (SA496_27340)	-	5820795..5821091 (-)	297	WP_120653590.1	DUF167 domain-containing protein	-
SA496_RS27345 (SA496_27345)	-	5821099..5821692 (-)	594	WP_120653589.1	YggT family protein	-
SA496_RS27350 (SA496_27350)	proC	5821703..5822521 (-)	819	WP_120653588.1	pyrroline-5-carboxylate reductase	-
SA496_RS27355 (SA496_27355)	-	5822532..5823227 (-)	696	WP_330114760.1	YggS family pyridoxal phosphate-dependent enzyme	-
SA496_RS27360 (SA496_27360)	pilT	5823391..5824425 (+)	1035	WP_028629028.1	type IV pilus twitching motility protein PilT	Machinery gene
SA496_RS27365 (SA496_27365)	pilU	5824453..5825598 (+)	1146	WP_330114761.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SA496_RS27370 (SA496_27370)	-	5825674..5826069 (+)	396	WP_120653585.1	TM2 domain-containing protein	-
SA496_RS27375 (SA496_27375)	-	5826215..5827594 (+)	1380	WP_330114762.1	pyridoxal-phosphate dependent enzyme	-
SA496_RS27380 (SA496_27380)	-	5827591..5828775 (+)	1185	WP_330114763.1	PLP-dependent aspartate aminotransferase family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42644.00 Da
• Isoelectric Point: 6.6393

>NTDB_id=928658 SA496_RS27365 WP_330114761.1 5824453..5825598(+) (pilU) [Pseudomonas sp. JS3066]
MEFEKLLRLMVEKGGSDLFVTAGVPPSMKVNGKIMPVTKNPMSPEMTRETVMGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLIGMVLRRIETTIPTIDELKLPEILKQLSLTKRGLVLFVGATGTGKSTSLAAMIGHRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAIAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLVADLIRKGEVHELKGLMKRSTEHGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLQHMSDGLSLQVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=928658 SA496_RS27365 WP_330114761.1 5824453..5825598(+) (pilU) [Pseudomonas sp. JS3066]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAAAAAGGCGGTTCCGACCTCTTCGTCACGGCGGGCGTGCCGCCATC
GATGAAGGTCAATGGCAAGATCATGCCGGTGACCAAGAACCCCATGTCGCCGGAAATGACGCGGGAAACCGTGATGGGCG
TGATGAATGAACAGCAGCGGCGCGACTTCGCAGAGAACCACGAGTGCAACTTCGCCATCAGTGCGCGGGGCATTGGTCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGATAGGCATGGTGTTGCGTCGGATCGAGACCACCATCCCCACCATCGA
CGAGCTGAAGCTGCCGGAAATCCTCAAGCAGCTGTCGCTGACCAAACGCGGCCTGGTGCTCTTCGTCGGCGCCACCGGTA
CCGGTAAGTCCACCTCTCTGGCGGCGATGATCGGCCACCGCAACAAGAACAGCAGCGGTCACATCATTTCCATCGAGGAC
CCGATCGAGTTCATCCACCAGCATCAGAGCTGCATCGTCACCCAGCGTGAAGTGGGCATCGACACCGAATCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCAGACGTGATCCTGATCGGCGAAGTGCGGACCCGCGAGACCATGGACCACG
CCATCGCCTTTGCGGAAACCGGTCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGTATC
ATCAACTTCTTCCCCGCCGATCGGCAGCAGCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGTCGCGCGGTGATCGAAGTACTGCTGAACACGCCGCTGGTCGCCGATCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGGGCCTGATGAAACGCTCTACCGAACACGGCATGCAGACCTTCGACCAGGCG
CTCTACAACCTCTATTCCCAGGGCGAGATCACCTACGAAGATGCGCTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAACCGATGGTGAGCACCTGCAGCACATGAGCGATGGTCTGTCGCTGCAGGTGAGCGAAG
AAGATCCGGGGCGCCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	90.289	100	0.903
pilU	Acinetobacter baylyi ADP1	64.146	93.701	0.601
pilU	Vibrio cholerae strain A1552	58.286	91.864	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.643	88.189	0.394