Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VSX76_RS15195 Genome accession   NZ_CP143311
Coordinates   2825033..2826070 (-) Length   345 a.a.
NCBI ID   WP_007146970.1    Uniprot ID   F5T284
Organism   Methylophaga thalassica strain IOM01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2820033..2831070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VSX76_RS15180 (VSX76_15175) - 2820543..2822315 (+) 1773 WP_330110430.1 methanol/ethanol family PQQ-dependent dehydrogenase -
  VSX76_RS15185 (VSX76_15180) - 2822883..2823719 (-) 837 WP_007146968.1 OmpA family protein -
  VSX76_RS15190 (VSX76_15185) pilU 2823879..2825021 (-) 1143 WP_007146969.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VSX76_RS15195 (VSX76_15190) pilT 2825033..2826070 (-) 1038 WP_007146970.1 type IV pilus twitching motility protein PilT Machinery gene
  VSX76_RS15200 (VSX76_15195) - 2826161..2826850 (+) 690 WP_330110431.1 YggS family pyridoxal phosphate-dependent enzyme -
  VSX76_RS15205 (VSX76_15200) proC 2826898..2827731 (+) 834 WP_273178654.1 pyrroline-5-carboxylate reductase -
  VSX76_RS15210 (VSX76_15205) - 2827724..2828293 (+) 570 WP_007146973.1 YggT family protein -
  VSX76_RS15215 (VSX76_15210) metX 2828358..2829506 (+) 1149 WP_330110432.1 homoserine O-succinyltransferase MetX -
  VSX76_RS15220 (VSX76_15215) metW 2829506..2830108 (+) 603 WP_007146975.1 methionine biosynthesis protein MetW -
  VSX76_RS15225 (VSX76_15220) pyrE 2830104..2830748 (+) 645 WP_040576707.1 orotate phosphoribosyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38405.06 Da        Isoelectric Point: 6.7281

>NTDB_id=928626 VSX76_RS15195 WP_007146970.1 2825033..2826070(-) (pilT) [Methylophaga thalassica strain IOM01]
MDITELLAFSAKNNASDLHVSSGEPPMIRVDGDIKRINLPAMEHKDVHAMIYDIMNDKQRKDFEEFLEADFSFEVPGLAR
FRVNAFNQNRGAAAVFRTIPSKVLSLEELGAPDIFKTIADNHRGIVLVTGPTGSGKSTTLAGMINYRNEKDNEHILTIED
PIEFVHQSKKCLINQREVHRDTHGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLMKKVGGGRIAAHEIMIGTPAIRNLIREDKVPQMYSAIQTGGAIGMQTLDQCL
QDLVRRQKVTKQEALPRAVNKDLFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=928626 VSX76_RS15195 WP_007146970.1 2825033..2826070(-) (pilT) [Methylophaga thalassica strain IOM01]
ATGGATATTACTGAATTGCTTGCGTTCAGCGCAAAAAACAATGCTTCGGATTTACATGTTTCGTCAGGTGAGCCGCCAAT
GATTCGTGTGGATGGTGATATCAAGCGCATTAATCTGCCGGCGATGGAACATAAAGATGTCCACGCGATGATTTATGACA
TTATGAATGATAAACAGCGTAAGGATTTTGAAGAATTTCTTGAGGCTGATTTTTCATTTGAAGTGCCGGGGCTGGCGCGT
TTTCGTGTTAATGCGTTTAATCAGAACCGTGGTGCTGCTGCCGTTTTCAGAACGATTCCATCAAAAGTACTCAGTCTGGA
AGAACTGGGCGCTCCCGATATTTTTAAAACCATCGCGGATAATCATCGCGGTATTGTGTTGGTCACCGGGCCTACAGGTT
CAGGTAAGTCCACTACGTTGGCCGGCATGATTAATTATCGAAATGAAAAAGATAATGAACATATTCTGACGATTGAAGAC
CCAATCGAATTTGTGCATCAAAGTAAAAAATGTTTGATTAATCAGCGTGAAGTGCATCGTGATACACATGGCTTTAGTGA
AGCACTTCGAAGTGCGCTGCGTGAAGATCCGGATATTATCCTGGTCGGTGAGTTACGTGATCTGGAGACAATCAGACTGG
CGCTGACAGCCGCAGAAACCGGGCATTTGGTGTTTGGTACCTTACATACATCGTCGGCAGCCAAAACTATTGACCGGATT
ATTGATGTTTTCCCTGCTGCGGAAAAAGACATGGTGCGCTCCATGTTGTCAGAATCATTACGTGCCGTTATTTCCCAGAC
ATTGATGAAGAAAGTGGGAGGCGGACGCATCGCTGCACATGAAATTATGATTGGTACTCCGGCTATCAGAAACCTGATTC
GAGAAGACAAAGTACCGCAGATGTACTCGGCTATTCAGACGGGCGGCGCTATTGGAATGCAAACCTTAGATCAGTGTTTG
CAGGATTTAGTCAGACGACAAAAGGTCACCAAGCAAGAGGCATTACCACGTGCTGTTAATAAAGACTTATTCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5T284

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Pseudomonas aeruginosa PAK

80.178

97.971

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

77.126

98.841

0.762
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Neisseria meningitidis 8013

69.388

99.42

0.69
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.471

96.522

0.69
  pilT Vibrio cholerae strain A1552

71.471

96.522

0.69
  pilT Neisseria gonorrhoeae MS11

69.096

99.42

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Acinetobacter baylyi ADP1

41.471

98.551

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.003

98.261

0.403
  pilU Vibrio cholerae strain A1552

40.708

98.261

0.4