Detailed information NTG0928625

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: VSX76_RS15190
• Genome accession: NZ_CP143311
• Coordinates: 2823879..2825021 (-)
• Length: 380 a.a.
• NCBI ID: WP_007146969.1
• Uniprot ID: F5T283
• Organism: Methylophaga thalassica strain IOM01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2818879..2830021

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VSX76_RS15170 (VSX76_15165)	-	2819030..2819743 (+)	714	WP_330110428.1	hypothetical protein	-
VSX76_RS15175 (VSX76_15170)	-	2819744..2820124 (-)	381	WP_330110429.1	hypothetical protein	-
VSX76_RS15180 (VSX76_15175)	-	2820543..2822315 (+)	1773	WP_330110430.1	methanol/ethanol family PQQ-dependent dehydrogenase	-
VSX76_RS15185 (VSX76_15180)	-	2822883..2823719 (-)	837	WP_007146968.1	OmpA family protein	-
VSX76_RS15190 (VSX76_15185)	pilU	2823879..2825021 (-)	1143	WP_007146969.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
VSX76_RS15195 (VSX76_15190)	pilT	2825033..2826070 (-)	1038	WP_007146970.1	type IV pilus twitching motility protein PilT	Machinery gene
VSX76_RS15200 (VSX76_15195)	-	2826161..2826850 (+)	690	WP_330110431.1	YggS family pyridoxal phosphate-dependent enzyme	-
VSX76_RS15205 (VSX76_15200)	proC	2826898..2827731 (+)	834	WP_273178654.1	pyrroline-5-carboxylate reductase	-
VSX76_RS15210 (VSX76_15205)	-	2827724..2828293 (+)	570	WP_007146973.1	YggT family protein	-
VSX76_RS15215 (VSX76_15210)	metX	2828358..2829506 (+)	1149	WP_330110432.1	homoserine O-succinyltransferase MetX	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42289.55 Da
• Isoelectric Point: 5.9197

>NTDB_id=928625 VSX76_RS15190 WP_007146969.1 2823879..2825021(-) (pilU) [Methylophaga thalassica strain IOM01]
MEIVTILKAAVKHDASDVFITAGRPVTLKVNGRMATLSQEALQDDEARDLVLSTMSDAQKKTFEREKECNYALETKGAGR
FRISALYQRNKIAMVMRRIKDEIPTIEEIHVPQVIKELSMTKRGLVIFVGATGSGKSTTLAAMIGYRNQNSTGHILTIED
PIEFDHKHAGCVVTQREVGIDTDSYEVALRNSLRQAPDVIMIGEIRTRETMDYGVAFAETGHLCLSTLHANNANQAMDRI
INFFPEERHQQLFLDLSLNLKAVIAQRLIPKKDGSGRAVAVEVLLNTPLIADLIEKGKIYEIKEVMKRSKELGMQTFDQA
VYDLYKAGDISYEEALRNADSANEVRLMIKLGMESGELAKDSSVSSLSLQAEPEDVIGRM

Nucleotide

• Length: 1143 bp

>NTDB_id=928625 VSX76_RS15190 WP_007146969.1 2823879..2825021(-) (pilU) [Methylophaga thalassica strain IOM01]
ATGGAAATTGTCACGATACTAAAAGCCGCTGTGAAACATGATGCCTCAGATGTATTTATTACCGCTGGCCGCCCGGTTAC
CTTGAAAGTGAATGGCCGAATGGCAACGCTCTCTCAGGAAGCACTGCAGGATGATGAGGCCAGAGATTTAGTACTGAGCA
CGATGTCAGATGCACAGAAAAAAACATTTGAACGTGAAAAAGAGTGTAATTATGCACTGGAGACTAAAGGTGCAGGTCGC
TTTCGTATCAGCGCGCTTTACCAGCGAAATAAAATTGCTATGGTCATGCGCCGTATCAAAGATGAAATTCCTACCATTGA
AGAAATTCATGTGCCTCAGGTCATCAAAGAATTATCGATGACAAAACGTGGCTTAGTCATATTTGTCGGGGCGACAGGCA
GTGGTAAGTCAACCACTTTGGCGGCGATGATCGGTTATCGCAATCAAAACAGTACAGGCCACATTCTAACGATTGAAGAT
CCTATTGAGTTTGATCATAAGCATGCCGGATGTGTGGTGACACAAAGGGAAGTGGGGATTGATACTGATTCGTATGAAGT
TGCTTTACGCAATTCACTGCGTCAGGCGCCGGATGTCATCATGATTGGTGAGATACGTACACGTGAGACCATGGATTACG
GTGTGGCCTTTGCAGAAACCGGCCATCTTTGTTTATCCACCTTACACGCTAATAATGCGAATCAGGCAATGGATAGGATT
ATCAACTTTTTCCCTGAAGAGCGTCATCAACAATTATTTTTGGATCTTTCATTAAATCTTAAAGCGGTTATTGCTCAGAG
ACTCATTCCTAAAAAGGATGGCTCGGGCAGGGCTGTTGCGGTTGAAGTACTTCTTAATACGCCGCTGATTGCTGATTTGA
TTGAGAAAGGCAAGATTTATGAAATCAAAGAAGTAATGAAACGTTCTAAAGAGCTTGGGATGCAGACGTTCGATCAGGCT
GTCTATGATTTATATAAAGCAGGTGATATTAGCTACGAAGAAGCTTTACGTAATGCCGATTCAGCCAATGAAGTACGCTT
AATGATCAAGCTAGGCATGGAGTCCGGCGAATTAGCCAAAGATAGTTCAGTTTCCAGTTTGTCATTACAAGCTGAACCCG
AAGATGTGATAGGGCGTATGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (6-271)

3D structure

• AlphaFold DB: F5T283

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60	98.684	0.592
pilU	Acinetobacter baylyi ADP1	58.659	94.211	0.553
pilU	Vibrio cholerae strain A1552	56.571	92.105	0.521
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.478	89.211	0.379
pilT	Pseudomonas aeruginosa PAK	40.708	89.211	0.363