Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U6151_RS00675 Genome accession   NZ_CP143257
Coordinates   147702..148739 (-) Length   345 a.a.
NCBI ID   WP_043572832.1    Uniprot ID   A0A2R2IQE9
Organism   Chromobacterium subtsugae isolate delta-vioS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 142702..153739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6151_RS00660 (U6151_00660) - 143107..144720 (-) 1614 WP_043572828.1 response regulator -
  U6151_RS00665 (U6151_00665) - 144832..146445 (-) 1614 WP_052257934.1 response regulator -
  U6151_RS00670 (U6151_00670) pilU 146469..147605 (-) 1137 WP_043572830.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U6151_RS00675 (U6151_00675) pilT 147702..148739 (-) 1038 WP_043572832.1 type IV pilus twitching motility protein PilT Machinery gene
  U6151_RS00680 (U6151_00680) - 148866..149561 (+) 696 WP_043572834.1 YggS family pyridoxal phosphate-dependent enzyme -
  U6151_RS00685 (U6151_00685) proC 149612..150415 (+) 804 WP_043572836.1 pyrroline-5-carboxylate reductase -
  U6151_RS00690 (U6151_00690) - 150425..151009 (+) 585 WP_043572839.1 YggT family protein -
  U6151_RS00695 (U6151_00695) - 151088..151393 (+) 306 WP_043572841.1 c-type cytochrome -
  U6151_RS00700 (U6151_00700) dksA 151677..152087 (+) 411 WP_021478430.1 RNA polymerase-binding protein DksA -
  U6151_RS00705 (U6151_00705) aroG 152221..153285 (+) 1065 WP_043572845.1 3-deoxy-7-phosphoheptulonate synthase AroG -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38246.93 Da        Isoelectric Point: 6.5047

>NTDB_id=928313 U6151_RS00675 WP_043572832.1 147702..148739(-) (pilT) [Chromobacterium subtsugae isolate delta-vioS]
MEISELLAFTVKNKASDLHLSAGLPPMIRVNGDIRRINLPPLDHHDVHDMVYDIMNDYQRKIFEDVYECDFSFELPGIAR
FRVNAFVQNRGMASVFRVIPSKVLSLEQLAAPKIFQEIAGYPRGLVLVTGPTGSGKSTTLAAMIDYVNENDFSHILTVED
PIEFVHESKKSLINQRELGLQTQSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESVRAVIAQTLLKSKDGQGRVAAHEIMIGTPAIRNLIRENKIAQINSMIQTGQQHGMQTLDQC
LAELVRRNMVSPGEARAKASNKDSF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=928313 U6151_RS00675 WP_043572832.1 147702..148739(-) (pilT) [Chromobacterium subtsugae isolate delta-vioS]
ATGGAAATTTCCGAGCTCCTGGCTTTTACCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCGGCCGGCTTGCCGCCGAT
GATACGGGTGAACGGCGACATCCGCCGCATCAACCTGCCGCCGCTCGACCACCATGACGTGCACGACATGGTGTACGACA
TCATGAACGACTACCAGCGCAAGATCTTCGAGGACGTCTACGAGTGCGACTTCTCGTTCGAGCTGCCCGGCATCGCCCGC
TTCCGCGTCAACGCCTTCGTGCAGAACCGCGGCATGGCGTCGGTGTTCCGGGTGATTCCCAGCAAGGTGCTGTCGCTGGA
ACAGCTGGCGGCGCCGAAGATCTTCCAGGAGATCGCCGGCTATCCGCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCACGCTTGCGGCGATGATAGACTATGTCAACGAAAACGACTTCTCGCACATTCTTACCGTGGAAGAC
CCGATCGAGTTTGTCCACGAAAGCAAGAAAAGCCTGATCAACCAGCGCGAGCTGGGCTTGCAGACCCAGAGCTTCGCCAA
CGCGCTGAAAAGCGCGCTGCGCGAGGACCCCGACGTGATCCTGGTCGGCGAGCTGCGCGACCTGGAAACGATACGGCTGG
CGCTGACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCATACCAGCAGCGCCGCCAAGACCATAGACCGCATC
GTCGACGTGTTCCCGGCCGGCGAAAAGGAAATGGTGCGCTCGATGCTGTCGGAATCGGTGCGGGCGGTGATCGCCCAGAC
GCTGCTGAAGTCCAAGGACGGCCAGGGGCGGGTGGCGGCGCACGAGATCATGATAGGCACGCCTGCGATCCGCAACCTGA
TCCGCGAAAACAAGATCGCGCAGATCAACTCGATGATACAAACCGGCCAGCAGCACGGCATGCAGACGCTGGACCAGTGC
CTGGCGGAGCTGGTGCGGCGCAATATGGTGTCGCCGGGCGAGGCGCGCGCCAAGGCCAGCAACAAGGATTCGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R2IQE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

72.754

100

0.728
  pilT Acinetobacter baumannii D1279779

72.754

100

0.728
  pilT Acinetobacter baumannii strain A118

72.754

100

0.728
  pilT Neisseria gonorrhoeae MS11

71.884

100

0.719
  pilT Neisseria meningitidis 8013

71.884

100

0.719
  pilT Pseudomonas aeruginosa PAK

71.304

100

0.713
  pilT Pseudomonas stutzeri DSM 10701

71.014

100

0.71
  pilT Acinetobacter baylyi ADP1

70.725

100

0.707
  pilT Legionella pneumophila strain Lp02

70.435

100

0.704
  pilT Legionella pneumophila strain ERS1305867

70.435

100

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.864

97.971

0.655
  pilT Vibrio cholerae strain A1552

66.864

97.971

0.655
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Vibrio cholerae strain A1552

41.493

97.101

0.403
  pilU Acinetobacter baylyi ADP1

40.173

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

42.593

93.913

0.4