Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U6151_RS00670 Genome accession   NZ_CP143257
Coordinates   146469..147605 (-) Length   378 a.a.
NCBI ID   WP_043572830.1    Uniprot ID   A0A2R2IQG0
Organism   Chromobacterium subtsugae isolate delta-vioS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 141469..152605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6151_RS00645 (U6151_00645) moaD 141825..142076 (+) 252 WP_043572823.1 molybdopterin converting factor subunit 1 -
  U6151_RS00650 (U6151_00650) moaE 142078..142560 (+) 483 WP_043572825.1 molybdopterin synthase catalytic subunit MoaE -
  U6151_RS00655 (U6151_00655) thpR 142577..143098 (-) 522 WP_043572826.1 RNA 2',3'-cyclic phosphodiesterase -
  U6151_RS00660 (U6151_00660) - 143107..144720 (-) 1614 WP_043572828.1 response regulator -
  U6151_RS00665 (U6151_00665) - 144832..146445 (-) 1614 WP_052257934.1 response regulator -
  U6151_RS00670 (U6151_00670) pilU 146469..147605 (-) 1137 WP_043572830.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U6151_RS00675 (U6151_00675) pilT 147702..148739 (-) 1038 WP_043572832.1 type IV pilus twitching motility protein PilT Machinery gene
  U6151_RS00680 (U6151_00680) - 148866..149561 (+) 696 WP_043572834.1 YggS family pyridoxal phosphate-dependent enzyme -
  U6151_RS00685 (U6151_00685) proC 149612..150415 (+) 804 WP_043572836.1 pyrroline-5-carboxylate reductase -
  U6151_RS00690 (U6151_00690) - 150425..151009 (+) 585 WP_043572839.1 YggT family protein -
  U6151_RS00695 (U6151_00695) - 151088..151393 (+) 306 WP_043572841.1 c-type cytochrome -
  U6151_RS00700 (U6151_00700) dksA 151677..152087 (+) 411 WP_021478430.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42410.58 Da        Isoelectric Point: 6.1405

>NTDB_id=928312 U6151_RS00670 WP_043572830.1 146469..147605(-) (pilU) [Chromobacterium subtsugae isolate delta-vioS]
MEKDQASKFIHDLLKHAIGKNASDIFISAEFPPAMKIDGKITPVAPQPLSAQQSKELVRAIMNDRQTEEFEAKKEANFAI
NPPGIGRFRVSAFVQQGMVGMVLRKINTDIPNFDQLNLPEVLKDIALIKRGLVIFVGGTGSGKSTSLAALVDWRNTHNQD
HIITVEDPIEYVHTHKKSIVTQREIGVDTENWEVALKNTLRQAPDVILMGEIRDRETMAYGLQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDLSLNMRSIISQRLVPHKSGRGRVAAVEILLNSPLVSDMVFKGEIAGLKEVMARSREAG
MQTFDQSLFELFEAELITYEDALRNADSINDLRLRIKLYSESGRNRDPLEGIDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=928312 U6151_RS00670 WP_043572830.1 146469..147605(-) (pilU) [Chromobacterium subtsugae isolate delta-vioS]
ATGGAAAAAGACCAGGCCTCCAAATTCATCCATGATCTGCTGAAGCACGCGATCGGCAAGAACGCCTCGGACATCTTCAT
TTCCGCCGAATTTCCGCCGGCGATGAAGATAGACGGCAAGATCACGCCGGTGGCGCCGCAGCCCTTGTCCGCGCAGCAGA
GCAAGGAGCTGGTGCGCGCCATCATGAACGACCGCCAGACCGAGGAGTTCGAGGCGAAGAAGGAAGCCAATTTCGCGATC
AACCCGCCCGGCATCGGCCGCTTCCGCGTCAGCGCCTTCGTCCAGCAGGGCATGGTGGGCATGGTGCTGCGCAAGATCAA
CACCGACATCCCCAACTTCGACCAGTTGAACCTGCCCGAGGTGCTGAAGGACATCGCGCTGATCAAGCGCGGCCTGGTGA
TCTTCGTGGGCGGCACCGGCTCCGGCAAGTCCACCTCGCTGGCGGCGCTGGTGGACTGGCGCAACACCCACAACCAGGAT
CACATCATCACGGTCGAAGACCCGATCGAATACGTGCATACCCACAAGAAGTCCATCGTCACCCAGCGCGAGATCGGCGT
CGACACCGAAAACTGGGAAGTCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAAACCATGGCCTACGGCCTGCAGTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCC
AACCAGGCGCTGGACCGCATTCTCAACTTCTTCCCGGAGGAGCGCCACCAGCAGGTGCTGATGGACCTGTCGCTGAACAT
GCGCTCCATCATTTCGCAGCGGCTGGTGCCGCACAAGTCCGGCCGCGGCCGGGTGGCGGCGGTGGAGATCCTGCTCAACA
GCCCGCTGGTGTCGGACATGGTGTTCAAGGGCGAGATCGCCGGCCTGAAGGAGGTGATGGCGCGCTCGCGCGAGGCCGGC
ATGCAAACTTTCGACCAGTCGCTGTTCGAGTTGTTCGAGGCCGAGCTGATCACCTACGAGGACGCGCTGCGCAATGCCGA
CTCCATCAACGACCTGCGCCTGAGAATCAAGCTCTACAGCGAAAGCGGCCGCAACCGCGACCCGCTGGAGGGCATCGACC
ACCTGGATATTGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R2IQG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60

92.593

0.556
  pilU Acinetobacter baylyi ADP1

54.918

96.825

0.532
  pilU Vibrio cholerae strain A1552

53.561

92.857

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas aeruginosa PAK

40.95

89.153

0.365