Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VXJ35_RS22505 Genome accession   NZ_CP143089
Coordinates   4923378..4924415 (-) Length   345 a.a.
NCBI ID   WP_054759731.1    Uniprot ID   -
Organism   Methylomonas sp. DH-1 strain CS40     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4918378..4929415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXJ35_RS22480 - 4919016..4919354 (-) 339 WP_064023280.1 hypothetical protein -
  VXJ35_RS22485 - 4919367..4919537 (-) 171 WP_157198267.1 hypothetical protein -
  VXJ35_RS22490 - 4919595..4920188 (-) 594 WP_064023281.1 recombinase family protein -
  VXJ35_RS22495 mltB 4921032..4922210 (-) 1179 WP_231914812.1 lytic murein transglycosylase B -
  VXJ35_RS22500 pilU 4922228..4923361 (-) 1134 WP_064023282.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VXJ35_RS22505 pilT 4923378..4924415 (-) 1038 WP_054759731.1 type IV pilus twitching motility protein PilT Machinery gene
  VXJ35_RS22510 - 4924591..4925613 (-) 1023 WP_064023283.1 SPFH domain-containing protein -
  VXJ35_RS22515 - 4925813..4926436 (+) 624 WP_064023284.1 TPM domain-containing protein -
  VXJ35_RS22520 - 4926433..4927941 (+) 1509 WP_064023285.1 TIM44-like domain-containing protein -
  VXJ35_RS22525 - 4928086..4928439 (-) 354 WP_064023286.1 YciI family protein -
  VXJ35_RS22530 - 4928436..4928945 (-) 510 WP_064023287.1 SRPBCC family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38324.18 Da        Isoelectric Point: 7.0362

>NTDB_id=928223 VXJ35_RS22505 WP_054759731.1 4923378..4924415(-) (pilT) [Methylomonas sp. DH-1 strain CS40]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPALNHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEELDAPKFFQELCTKPRGLILVTGPTGSGKSTTLAAMINHINNNDYAHILTVED
PIEFVHESQKSLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRAMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGLITAKAAMTRAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=928223 VXJ35_RS22505 WP_054759731.1 4923378..4924415(-) (pilT) [Methylomonas sp. DH-1 strain CS40]
ATGGATATCGCCGAACTGCTGACGTTTTCCGTCAAGAATAAAGCCTCCGACTTGCACCTTTCCGCCGGTTTGCCGCCGAT
GATACGGGTGGACGGCGATATTCGCCGGATCAACATCCCGGCGCTGAATCATAAGGAAGTGCATGCGTTGATTTACGACA
TCATGAACGACAAACAGCGCCGCGACTACGAAGAGTTTCTGGAAACCGACTTTTCCTTCGCCTTGCCCGGCGTGGCCCGG
TTCCGGGTCAACGCCTTCAACCAGGACCGCGGCGCCGGCGCGGTATTCCGCACCATTCCGTCGAAAGTCCTGACCTTGGA
AGAGCTGGATGCACCCAAGTTTTTCCAGGAATTGTGTACCAAACCGCGCGGCTTGATCCTGGTGACCGGACCGACCGGTT
CCGGTAAATCCACCACGTTGGCGGCGATGATCAACCACATCAACAACAACGATTATGCCCACATCCTGACCGTCGAGGAC
CCGATCGAGTTCGTCCACGAAAGCCAGAAGTCGCTGATCAACCAGCGCGAAGTCCACCGCGACACGCTAGGATTCAACGA
AGCGTTGCGTTCTGCTTTGCGGGAAGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATTCGTTTGG
CGCTGACCGCGGCCGAAACCGGCCACTTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATAGACCGGATT
ATCGACGTGTTCCCGGCCGCCGAGAAAGACATGATCCGCGCCATGCTATCGGAATCGTTGCAGGCGGTTATTTCGCAAAC
GCTGTTGAAGAAAGTCGGGGGCGGCCGGATCGCTGCGCACGAGATCATGGTCGGTACCCCGGCGATCCGCAACCTGATCC
GCGAAGCCAAGGTGGCACAGATGTATTCGGCGATTCAAACCGGGCGCAAGGACGGCATGCAGACGCTGGACCAAAATTTA
AAAGAGTTGGTCGACAAGGGCCTGATTACCGCCAAAGCGGCGATGACCCGAGCCGTCAATAAAGACATGTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

81.232

98.841

0.803
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

80.473

97.971

0.788
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.681

0.678
  pilT Vibrio cholerae strain A1552

69.436

97.681

0.678
  pilT Neisseria gonorrhoeae MS11

67.638

99.42

0.672
  pilT Neisseria meningitidis 8013

67.638

99.42

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Acinetobacter baylyi ADP1

40.346

100

0.406
  pilU Vibrio cholerae strain A1552

40.708

98.261

0.4
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4